Impact of pH, ionic strength and chitosan charge density on chitosan/casein complexation and phase behavior

Ding, Lan; Huang, Yan; Cai, Xiaoyan; Wang, Shaoyun

doi:10.1016/j.carbpol.2018.12.015

Cited by 126 publications

(61 citation statements)

References 53 publications

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“…This might be due to the increased interaction between EPS and CAS molecules to form more macromolecular complexes as the strength of the force between the protein molecules reduced [ 38 ]. Electrostatic interaction was found to be the major driving force for polysaccharide/CAS complexation, and its particle size was dependent on the concentration of the polysaccharide [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…FT-IR spectroscopy is an effective method to evaluate the binding interaction for complex formation and the secondary structure changes in CAS after the addition of polysaccharide [ 24 ]. As shown in Figure 2 D, the FT-IR spectrum of the EPS-YW11/CAS complex samples demonstrates a broad peak at 3396 cm −1 , which is attributed to the strong –OH stretching vibration absorption [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

“…To prepare TEM samples, one drop of the complex suspension was placed on a copper grid and stained with 2% phosphotungstic acid, which was then air dried overnight. The grid was placed in the microscope for imaging at 100 kV accelerating voltage, while the images were taken on a Gatan electron energy loss spectrometry system using a 6 eV energy slit [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Interaction of the Exopolysaccharide from Lactobacillus plantarum YW11 with Casein and Bioactivities of the Polymer Complex

Zhang

Lai

Yao

et al. 2021

Foods

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There has been an increased application of exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) in fermented dairy products, but interactions between EPS and casein (CAS), and bioactivities of their complex are poorly studied. In this study, EPS produced by Lactobacillus plantarum YW11 (EPS-YW11) was studied for interactions with CAS in a simulated fermentation system acidified by D-(+)-gluconic acid δ-lactone. The results showed that there was interaction between EPS-YW11 and CAS when EPS (up to 1%, w/v) was added to the casein solution (3%, w/v) as observed with increased viscoelasticity, water holding capacity, ζ-potential and particle size of EPS-YW11/CAS complex compared with CAS alone. Microstructural analysis showed that a higher concentration of EPS facilitated more even distribution of CAS particles that were connected through the polysaccharide chains. Infrared spectroscopy further confirmed interactions between EPS and CAS by intermolecular hydrogen bonding, electrostatic and hydrophobic contacts. Further evaluation of the bioactivities of EPS-YW11/CAS complex revealed significantly increased antibiofilm, antioxidation, and bile acids binding capacity. The present study provides further understanding on the mechanism of interactions between EPS produced by LAB and CAS, which would benefit potential applications of EPS in fermented dairy products with enhanced functionality.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Interaction of the Exopolysaccharide from Lactobacillus plantarum YW11 with Casein and Bioactivities of the Polymer Complex

Zhang

Lai

Yao

et al. 2021

Foods

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“…It has been reported that high M W and low DA values are associated with an improvement in antimicrobial activity [15]. Chitosan with a lower DA exhibits higher antimicrobial activity, due to a high positive charge that allows chitosan to dilute in acidified media [16,17]. Meanwhile, M W influences the flexibility of the functional groups that interact with the microbial cells [18].…”

Section: Introductionmentioning

confidence: 99%

Structural and Physicochemical Characterization of Chitosan Obtained by UAE and Its Effect on the Growth Inhibition of Pythium ultimum

Martín-López¹,

Pech-Cohuo²,

Herrera-Pool³

et al. 2020

Agriculture

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The objective of this work was the recovery of chitosan by ultrasound-assisted extraction (UAE) from white shrimp (Litopenaeus vannamei) chitin, and the physicochemical and structural characterization of the obtained biopolymer, as well as its antimicrobial effect on Pythium ultimum growth. A 23 factorial design was used to evaluate chitosan extraction conditions. Instrumental analysis techniques for chitosan characterization and radial growth inhibition, as an antifungal activity test, were performed. The ultrasonically extracted chitosan (UC) reached a yield of 86.96% with 100% solubility, a degree of deacetylation (DDA) >78%, molecular weight (MW) of 3.928 × 105 g mol−1, and a crystallinity index (Icr) of 87%, calculated through nuclear magnetic resonance (1H NMR) and Fourier transform infrared spectroscopy (FTIR), size exclusion chromatography (SEC), and X-ray diffraction (XRD), respectively. The inhibitory activity of the chitosan was evaluated against the oomycete Pythium ultimum, observing a 93% radial inhibition over 24 h. UAE proved to be an excellent alternative to the conventional deacetylation, reducing reaction time and obtaining a UC with higher MW and (Icr) than the commercial one, which could potentiate its applications.

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“…The detailed preparation process was shown in the Scheme 1. Generally, the 1-butyl-3-methyllimidazolium chloride was suitable to be used in the conversion of casein, which was rich in nitrogen, into porous carbon materials (Jia et al, 2017; Ding et al, 2019). The ionic liquids acted as a reaction medium and a porosity-directing regulator (Lee et al, 2010; Guo et al, 2013; Zhang et al, 2015c; Martinaiou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Co-N-C Catalyst Synthesized With a Hard-Template and Etching Method to Achieve Well-Dispersed Active Sites for Ethylbenzene Oxidation

et al. 2019

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Biomass obtained from organic residues gradually becomes one of the optimal renewable feedstock of value added chemicals. Herein, the Co-N-C catalyst was prepared via a hard-template and etching method using the casein as C and N sources, magnesium oxide as the template, and cobalt porphyrin as the metal precursor. The obtained Co-N-C catalyst exhibited excellent catalytic performance for selective oxidation of ethylbenzene with a conversion rate of 96.5% under mild conditions. Moreover, the catalysts were investigated by techniques such as BET, XRD, Raman, transmission electron microscopic (TEM), and X-ray photoelectron spectroscopy (XPS). The results showed that the etching progress could improve the dispersion of Co and the exposure of active sites. Herein, the efficient oxidation of ethylbenzene was attributed to the well-dispersed Co-N species and the increased specific surface area.

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Impact of pH, ionic strength and chitosan charge density on chitosan/casein complexation and phase behavior

Cited by 126 publications

References 53 publications

Interaction of the Exopolysaccharide from Lactobacillus plantarum YW11 with Casein and Bioactivities of the Polymer Complex

Interaction of the Exopolysaccharide from Lactobacillus plantarum YW11 with Casein and Bioactivities of the Polymer Complex

Structural and Physicochemical Characterization of Chitosan Obtained by UAE and Its Effect on the Growth Inhibition of Pythium ultimum

The Co-N-C Catalyst Synthesized With a Hard-Template and Etching Method to Achieve Well-Dispersed Active Sites for Ethylbenzene Oxidation

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