Impact of pH and temperature on phase diagrams of different aqueous biphasic systems

Chakraborty, Arabinda; Sen, Kamalika

doi:10.1016/j.chroma.2016.01.016

Cited by 57 publications

(42 citation statements)

References 152 publications

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“…At high pH values, these anions are less protonated, have higher valence, and are better salting‐out agents as they have a more negative Gibbs free energy of hydration. Thus, the ions in the basic medium are more efficient in promoting the phase formation compared with those with lower valence in an acidic medium . This effect has been reported for binodal curves composed of ionic liquid C 4 mim Br (1‐butyl‐3‐methylimidazolium bromide) + potassium citrate/citric acid …”

Section: Resultsmentioning

confidence: 74%

“…Thus, the ions in the basic medium are more efficient in promoting the phase formation compared with those with lower valence in an acidic medium. 26 This effect has been reported for binodal curves composed of ionic liquid C 4 mim Br (1-butyl-3-methylimidazolium bromide) + potassium citrate/citric acid. 27 Regarding the cation effect (either potassium or sodium) with EMIM Ac at pH 7.0, there was no clear difference in the phase diagrams ( Fig.…”

Section: Binodal Curvesmentioning

confidence: 62%

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Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

González‐Mora

Ruiz‐Ruiz

Benavides

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Bacteriophage M13 is an Escherichia coli‐specific non‐lytic filamentous virus commonly used in applications ranging from antibody screening and nanomaterial construction to drug delivery, among others. In this tenor, alternative methods for the fractionation, recovery and partial purification of phage particles are desired. In this work, the use of aqueous two‐phase systems (ATPS) was evaluated as an alternative method for the recovery of phage particles. RESULTS The partition behavior of M13 in PEG–salt and ionic liquid (IL)–salt ATPS was characterized using a pre‐purified feedstock. In PEG‐salt ATPS, M13 was preferentially partitioned to the interface. In IL ATPS, however, M13 showed a high‐top phase preference with recovery yields above 65%. Selected systems were tested for the extraction of M13 from a crude fermentation broth. From crude broth, a PEG 400‐potassium phosphate system with volume ratio (VR) of 1 and 25% w/w tie line length (TLL) gave the best M13 top phase recovery (83%) and purification fold (18.2) in terms of total protein concentration. CONCLUSIONS The results presented here demonstrate the practical application of ATPS as an efficient process for the primary recovery and partial purification of M13 and represent the first study of the extraction of viral particles directly from a crude broth as well as the use of IL‐Salt ATPS. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 74%

Section: Binodal Curvesmentioning

confidence: 62%

Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

González‐Mora

Ruiz‐Ruiz

Benavides

et al. 2017

J of Chemical Tech & Biotech

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“…The partitioning of polyhydroxyalkanoate (PHA) from Cupriavidus necator H-16 in the thermoseparating-based LBS showed a good setup in altering the pH system as compared with conventional PEG-based LBS [89]. PHA showed a purification factor and recovery yield of 3.67% and 63.5%, respectively, at the pH 6 which was better than the conventional PEG-based LBS that had zero recovery of PHA in the top phase when pH was less than 7.…”

Section: Ph Systemmentioning

confidence: 99%

“…However, the studies showed that the extraction efficiency of both BSA and papain decreased when the temperature was increased. This phenomenon was due to the increasing temperature which could inhibit the interaction of amino acid and surface water of protein, resulting in less efficiency of protein extraction [89]. Hence, the effect of temperature should be taken into consideration as the extraction efficiency of the biomolecule is dependent on the range of temperature in the LBS.…”

Section: Temperaturementioning

confidence: 99%

Liquid Biphasic System: A Recent Bioseparation Technology

et al. 2020

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A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.

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“…These include the study on [C 4 py]Br/H 2 O/ Na 2 SO 4 at various temperatures by Li and coworkers [138] and that on cholineamino acid ionic liquids/K 2 CO 3 /H 2 O by Li and coworkers [139], as well as the analysis on an extensive range of these ionic liquid-based aqueous biphasic solvent systems by Sen and Chakraborty [140]. [125].…”

Section: Ionic Liquid-water Systemsmentioning

confidence: 99%

Ionic Liquid–Liquid Chromatography: A New General Purpose Separation Methodology

Brown¹,

Earle

Gîlea

et al. 2017

Topics in Current Chemistry Collections

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Ionic liquids can form biphasic solvent systems with many organic solvents and water, and these solvent systems can be used in liquid-liquid separations and countercurrent chromatography. The wide range of ionic liquids that can by synthesised, with specifically tailored properties, represents a new philosophy for the separation of organic, inorganic and bio-based materials. A customised countercurrent chromatograph has been designed and constructed specifically to allow the more viscous character of ionic liquid-based solvent systems to be used in a wide variety of separations (including transition metal salts, arenes, alkenes, alkanes, bio-oils and sugars).

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Impact of pH and temperature on phase diagrams of different aqueous biphasic systems

Cited by 57 publications

References 152 publications

Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

Liquid Biphasic System: A Recent Bioseparation Technology

Ionic Liquid–Liquid Chromatography: A New General Purpose Separation Methodology

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