Impact of Pesticides on Environmental and Human Health

“…Los componentes de las formulaciones de los plaguicidas usados como polvos humectables consisten usualmente en el vehí-culo, arcillas minerales, agentes tensioactivos y otros ingredientes tales como estabilizadores y tintoreros. La presencia de agentes tensioactivos podría aumentar la actividad genotóxica del I.A., favoreciendo la cantidad de metabolitos activos en objetivos celulares críticos (Bernardes et al 2015).…”

Section: Discussionunclassified

INDUCCIÓN DE MICRONÚCLEOS EN CÉLULAS MERISTEMÁTICAS DE LA RAÍZ DE Vicia faba TRATADAS CON DIFERENTES CONCENTRACIONES DE MARVEL®

Alarcón

Pérez-Sánchez

Gómez-Olivares

et al. 2018

Rev. Int. Contam. Ambie.

View full text Add to dashboard Cite

Palabras clave: Micronúcleos, atrazina, dicamba, índice mitótico RESUMEN El empleo de plaguicidas ha ido aumentando al paso del tiempo. A pesar de los beneficios aportados por los agroquímicos tanto en la agricultura como en el ámbito doméstico, muchos de ellos pueden representar peligros potenciales para la salud y el ambiente. En la actualidad es manifiesto el interés creciente en la determinación de biomarcadores que permitan la medición y estimación de una exposición activa y/o pasiva a contaminantes ambientales con capacidad tóxica, como los plaguicidas. Este estudio analiza el daño al ADN que causa el herbicida comercial Marvel® a través del ensayo de micronúcleos (MN), en células meristemáticas de la raíz de Vicia faba. Raíces de entre 2-3 cm fueron expuestas durante 4 horas, con 18 y 44 horas de recuperación a diferentes concentraciones de Marvel®, equivalentes a las siguientes combinaciones de atrazina/dicamba: 62.5/31.25; 125/62.5; 250/125; 1000/400; 2000/1000; 3000/1500 y 4000/2000 mg de ingrediente activo/L de agua destilada (mg I.A./L). Las células fueron expuestas a agua destilada, como testigo negativo y a dicromato de potasio 0.05%, como testigo positivo, bajo las mismas condiciones experimentales. Con excepción de la concentración más baja [62.5/31.25 mg I.A./L], todas las concentraciones probadas presentaron un daño significativo (p<0.05). Con respecto al testigo negativo, el daño fue mayor a las 18 horas de recuperación, al paso de 44 horas, el daño se vio disminuido en todas las concentraciones probadas. El índice mitótico (IM) también se modificó, mostrándose una menor división dependiente de la concentración y tiempo de recuperación. Las concentraciones de 2000/1000; 3000/1500 y 4000/2000, mg I.A./L, produjeron un daño mayor que el encontrado en el testigo positivo. Los datos sugieren que el herbicida comercial Marvel® produce daño al ADN y es citotóxico en Vicia faba.

show abstract

“…In this spectral region are also expected stretching vibrations localized in the imidazole ring: two medium-intensity Raman bands centered at 1,528 and 1,512 cm −1 have been published for methyl imidazole and at 1,535 and 1,488 cm −1 for imidazole in neutral state, which have been briefly reported as ring vibrations, [32,33] and a medium-intensity band at 1,504 cm −1 has been observed in an N-substituted imidazole product and attributed to the stretching of the single C-N bond. [34] According to our calculations for the imazalil molecule, these vibrations can be characterized as the out-of-phase stretching of the C═C and C═N (2) bonds (ν o.o.ph C═C/C═N (2) ) and the symmetric stretching ν s CN (1) C, which are predicted at 1,496 and 1,484 cm −1 and would be observed as the intense FTIR features at 1,502 and 1,469 cm −1 , respectively. This last band, not observed in the Raman spectra of imazalil, could be also attributed to a deformation mode of the CH 2 ether group, calculated at 1,464 cm −1 (see Table 2).…”

Section: Vibrational Characterization Of Imazalilmentioning

confidence: 92%

“…Table 3 lists the SERS bands shown in Figure 4 together with the corresponding wavenumbers predicted for the most probable imazalil-Ag complex. According to the observed and calculated features, it is proposed that the band originated by the ν o.o.ph C═C/C═N (2) mode experiences an important upshift upon SERS effect (from 1,504 to 1,529 or 1,547 cm −1 ), and the irregular band contour on the low wavenumber side indicates that the ν s CN (1) C mode becomes more active. These interpretations are in concordance with the expected interaction of the imazalil molecule with the metal surface: The corresponding wavenumbers for both vibrations in Complex I are predicted experiencing blueshifts.…”

Section: Sers Spectra Of Imazalil On Si/zno/ Agnp Substratementioning

confidence: 99%

SERS spectrum of imazalil. Experimental and quantum‐chemical vibrational analysis

Díaz-Mirón

Sánchez

Chemes

et al. 2017

J Raman Spectroscopy

View full text Add to dashboard Cite

Imazalil [1‐[2‐(2,4‐dichlorophenyl)‐2‐(2‐propenyloxyl‐ethyl]‐1 H imidazole] is a systemic fungicide that is toxic to a number of plant pathogens, particularly species of Penicillum. Its activity is based on the inhibition of mold sporulation on fruit skin, which makes it one of the most commonly used fungicide in post‐harvest citrus treatments for fruit preservation during storage, shipping, and marketing. Due to its wide application, the presence of imazalil needs to be monitored to avoid the excessive and/or improper use. This work reports a complete analysis of the Fourier‐transform infrared spectroscopy and Raman spectra of imazalil as a pure compound as well as in the commercial product for agricultural use; the band assignment relays in the vibrational predictions acquired by quantum‐chemical calculations (B3LYP/6–311 + g(2df, p)) for the imazalil molecule. An analytical application of surface‐enhanced Raman spectroscopy (SERS) is also presented here. It is done so by using a novel, low‐cost, and sensitive SERS‐active substrate built with silver nanoparticles supported on a Si/ZnO nanowires platform to detect traces of imazalil in aqueous solutions. Comparison between the Raman and SERS spectra allowed the characterization of the interaction between the pesticide and the silver nanoparticle surface.

show abstract

Impact of Pesticides on Environmental and Human Health

Cited by 134 publications

References 117 publications

Evaluation of genotoxic and cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura

Evaluation of genotoxic and cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura

INDUCCIÓN DE MICRONÚCLEOS EN CÉLULAS MERISTEMÁTICAS DE LA RAÍZ DE Vicia faba TRATADAS CON DIFERENTES CONCENTRACIONES DE MARVEL®

SERS spectrum of imazalil. Experimental and quantum‐chemical vibrational analysis

Contact Info

Product

Resources

About