Impact of oral bisphenol A at reference doses on intestinal barrier function and sex differences after perinatal exposure in rats

Abstract

Bisphenol A (BPA), a chemical estrogen widely used in the foodpackaging industry and baby bottles, is recovered in human fluids (0.1-10 nM). Recent studies have reported that BPA is hormonally active at low doses, emphasizing the debate of a risk for human health. Estrogen receptors are expressed in the colon, and although the major route of BPA exposure is food, the effects on gut have received no attention. We first examined the endocrine disrupting potency of BPA on colonic paracellular permeability (CPP), experimental colitis, and visceral sensitivity in ovariectomized rats orally exposed to 5 mg/kg/d BPA (i.e., the no observed adverse effect level), 50 μg/kg/d BPA (i.e., tolerable daily intake), or lower doses. BPA dosedependently decreased basal CPP, with a half-maximal inhibitory dose of 5.2 μg/kg/d, 10-fold below the tolerable daily intake. This correlated with an increase in epithelial tight junction sealing, also observed in Caco-2 cells exposed to 10 nM BPA. When ovariectomized rats were fed with BPA at the no observed adverse effect level, the severity of colitis was reduced, whereas the same dose increased pain sensitivity to colorectal stimuli. We then examined the impact of perinatal exposure to BPA on intestinal permeability and inflammatory response in the offspring. In female rats, but not in male rats, perinatal BPA evoked a decrease of CPP in adulthood, whereas the proinflammatory response of colonic mucosa was strengthened. This study first demonstrates that the xenoestrogen BPA at reference doses influences intestinal barrier function and gut nociception. Moreover, perinatal exposure promotes the development of severe inflammation in adult female offspring only.endocrine disruptor | gut permeability | inflammation | pain | estrogen

Influence of bisfenol A on chemical coding of the nerve fibers of the cardiac apex in the domestic pig

“…Dotychczas wykazano, iż bisfenol A może wpływać negatywnie na wiele narządów i układów, szczególnie w przypadku osobników mło-dych (25,26). Wcześniejsze badania opisują, iż działa on neurotoksycznie, kancerogennie, uszkadza układ rozrodczy, hormonalny, pokarmowy, immunologiczny oraz sercowo-naczyniowy (5,14,25,29). Ponadto wiadomo, że BPA po dostaniu się do organizmu może być kumulowany w wielu tkankach, a następnie powoli uwalniany, co powoduje przewlekłą intoksykację nawet po ustaniu bezpośredniej ekspozycji na ten związek.…”

Estrogen Deficiency Leads to Impaired Osteogenic Differentiation of Periodontal Ligament Stem Cells in Rats

“…NM 012587), and GAPDH (GenBank accession no. NM 017008) were as follows: (1) ERα-Sense, 5ʹ-GGC CCT CCC GCC TTC TA -3ʹ, and ERα-antisense, 5ʹ-GGA GAG TCT CTC TCG GCC ATT -3ʹ; (2) ERβ-Sense, 5ʹ-AAC CTC CTG ATG CTT CTT TCT CAC -3ʹ, and ERβ-antisense, 5ʹ-CTT CAT GCT GAG CAG ATG TTC C-3ʹ (Braniste et al 2010); (3) ALP-Sense, 5ʹ-AAC GTG GCC AAG AAC ATC ATC A-3ʹ, and ALP-antisense, 5ʹ-TGT CCA TCT CCA GCC GTG TC-3ʹ; (4) BSP-Sense, 5ʹ-TGT GGA ATG GTG CTA CGG TCT C-3ʹ, and BSP-antisense, 5ʹ-GAT CAA CAG CCC TGA TTT ACG ATG-3ʹ; (5) GAPDH-Sense, 5ʹ-GAC AAC TTT GGC ATC GTG GA-3ʹ, and GAPDH-antisense, 5ʹ-ATG CAG GGA TGA TGT TCT GG-3ʹ used as an internal control. Relative quantization was done by using the ∆Ct method by taking the difference (∆Ct) between the ∆Ct of GAPDH and ∆Ct of each transcript and computing ∆Ct.…”

“…The sequences of primers for ERβ, ALP, BSP, and GAPDH were designed by Takara (Bio Co Ltd, Kyoto, Japan). The amplification condition for ALP, BSP, and GAPDH was denaturing at 95°C for 30 s, 35 cycles at 95°C for 10 s, and at 64°C for 30 s. The amplification condition for ERα and ERβ was as follows: 95 °C for 10 min, 40 cycles at 95°C for 15 s and 60°C for 30 s (Ruijter et al 2009;Braniste et al 2010). Triplicate reaction and 3 separate experiments were performed.…”

Effects of exposure to bisphenol A during pregnancy and lactation on the testicular morphology and caspase-3 protein expression of ICR pups

“…BPA is one of the most widely spread endocrine-disrupting chemicals, which can be found in the carbohydrate-rich foods; thus, BPA was classified as a probable human carcinogen (4)(5)(6). In their study, Braniste et al (7) demonstrated that BPA induced estrogen-like activities in the intestine of rats orally exposed to carcinogens. No adverse effects were observed, while BPA decreased the basal epithelial permeability of the colon and strengthened nociceptive responses by binding to estrogen receptors (ERs).…”