Impact of nonsteroidal aromatase inhibitors on steroid profile in a Chinese population

Abstract

Supplemental Digital Content is available in the text

Detection of urinary metabolites of arimistane in humans by gas chromatography coupled to high‐accuracy mass spectrometry for antidoping analyses

“…For that reason, proposed as good markers were the ratios A/Σ estrogens and Et/Σ estrogens. A study of 41 female and male Chinese subjects was performed by administering aminoglutetimide, anastrazol and letrozol, and it was also concluded that there was no common trend among the ABP biomarkers and the best markers were those related to estrogens …”

“…19 A study of 41 female and male Chinese subjects was performed by administering aminoglutetimide, anastrazol and letrozol, and it was also concluded that there was no common trend among the ABP biomarkers and the best markers were those related to estrogens. 20 The variations of the parameters of the steroid module of the ABP did not exceed 30% except for 5βAdiol for volunteer 1. It is worth mentioning that the time of collection was for more than two circadian cycles, so these variations obviously are influenced by physiological fluctuation.…”

“…The influence of the administration of aromatase inhibitors on the parameters of the steroidal module of the ABP has been studied by various research groups. [18][19][20] One of them described the influence of oral and multiple doses of tamoxifene, toremifene and clomifene and the conclusions were that, of the endogenous profile parameters of the ABP, only the T and E urinary concentrations increased significantly in males, generally after the second dose, while no significant variations were measured for the urinary concentrations of A, Etio, 5αAdiol and 5βAdiol and the ratios T/E, A/Etio and 5α/5β-Adiols. 18 Another experiment administering multiple doses of clomifene showed that the A/Etio ratio and its absolute concentrations were the most stable parameters while the T/E ratio decreased not significantly on the third day of treatment and remained very stable until the end of the study.…”

Hair analysis to discriminate voluntary doping vs inadvertent ingestion of the aromatase inhibitor letrozole

“…8 However, if, despite the lack of evidence, it is deemed to be true that letrozole is capable of somehow increasing testosterone and/or androstenedione levels in premenopausal female athletes, when they use this AI, then their serum steroid profiles would become somehow altered or disrupted. 38,39 In particular, their androgen levels should increase with respect to the normal range and their estrogens should be suppressed. If this is not true, then there is no reliable evidence to suggest that letrozole is capable of having a doping effect.…”

Annual banned‐substance review: Analytical approaches in human sports drug testing

“…The assay's LODs ranged from 0.1 ng/mL to 20 ng/mL, demonstrating the methods ability to quantifying a total of 22 urinary steroids. The utility of a steroid profile that includes estrogens (in addition to the commonly monitored androgens) to indicate the misuse of non‐steroidal aromatase inhibitors was assessed by Xing et al While aromatase inhibitors such as aminoglutethimide, letrozole, and anastrozole are efficiently analyzed by routine targeted LC–MS(/MS) methods, complementary information derived from the steroid profile may assist in detecting their misuse. In a study including 20 male and 21 female volunteers, either aminoglutethimide (2 × 250 mg/day, 3 days), letrozole (2.5 mg/day, 5 days), or anastrozole (1 mg/day, 5 days) were administered orally.…”