Impact of nanometer-scale roughness on contact-angle hysteresis and globulin adsorption

Müller, Bert; Riedel, Marco; Roger, Michel; Paul, Susan M. De; Hofer, R.; Heger, D.; Grützmacher, Detlev

doi:10.1116/1.1392402

Cited by 94 publications

(73 citation statements)

References 73 publications

(50 reference statements)

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“…Yet, roughness is only a single measure of the topography of a surface, and despite many studies there appears to be no single unifying description of how roughness affects binding (1,2,(52)(53)(54). Our experiments are performed on surfaces with rms roughness between 1.1 nm and 0.18 nm (PD and SCD), considerably smaller than the rms roughness in most other studies (53,55). In addition, the length scale over which the roughness occurs may be important.…”

Section: Discussionmentioning

confidence: 99%

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Stavis

Clare

Butler

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Carbon is an extremely versatile family of materials with a wide range of mechanical, optical, and mechanical properties, but many similarities in surface chemistry. As one of the most chemically stable materials known, carbon provides an outstanding platform for the development of highly tunable molecular and biomolecular interfaces. Photochemical grafting of alkenes has emerged as an attractive method for functionalizing surfaces of diamond, but many aspects of the surface chemistry and impact on biological recognition processes remain unexplored. Here we report investigations of the interaction of functionalized diamond surfaces with proteins and biological cells using X-ray photoelectron spectroscopy (XPS), atomic force microscopy, and fluorescence methods. XPS data show that functionalization of diamond with short ethylene glycol oligomers reduces the nonspecific binding of fibrinogen below the detection limit of XPS, estimated as >97% reduction over H-terminated diamond. Measurements of different forms of diamond with different roughness are used to explore the influence of roughness on nonspecific binding onto H-terminated and ethylene glycol (EG)-terminated surfaces. Finally, we use XPS to characterize the chemical stability of Escherichia coli K12 antibodies on the surfaces of diamond and amine-functionalized glass. Our results show that antibody-modified diamond surfaces exhibit increased stability in XPS and that this is accompanied by retention of biological activity in cell-capture measurements. Our results demonstrate that surface chemistry on diamond and other carbonbased materials provides an excellent platform for biomolecular interfaces with high stability and high selectivity.biointerfaces | surface chemistry | cells

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Section: Discussionmentioning

confidence: 99%

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Stavis

Clare

Butler

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Hysteresis is influenced by many factors, including the surface roughness, chemical heterogeneity of the surface, presence of low molecular weight species, and molecular orientation. 49 In a multiphase system advancing contact angle is more sensitive to the low surface energy or hydrophobic domains whereas receding contact angle is more sensitive to the high surface energy or hydrophilic domains. 50 We attribute the increased hysteresis in the POSS containing systems to the surface roughness as well as the chemical heterogeneity imparted by the enrichment of Oib-POSS nanoparticles on the PP surface.…”

Section: Resultsmentioning

confidence: 99%

Surface energetics, dispersion, and nanotribomechanical behavior of POSS/PP hybrid nanocomposites

Misra¹,

Fu²,

Morgan³

2007

J Polym Sci B Polym Phys

107

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Hybrid organic-inorganic polymer nanocomposites incorporating polyhedral oligomeric silsesquioxane (POSS) nanoparticles are of increasing interest for high performance materials applications. Octaisobutyl POSS/polypropylene nanocomposites were prepared at varying POSS concentrations via melt blending. The interplay of POSS molecular geometry, composition, and concentration in relation to the tribological, nanomechanical, surface energy, and bulk properties of the nanocomposites were investigated. Ultra-low friction and enhanced hardness, modulus, and hydrophobicity were observed for the nanocomposite surfaces, with minimal changes in the bulk thermomechanical properties. Parallel AFM, SEM, TEM, and spectroscopic analyses demonstrated significant differences in POSS distribution and aggregation in the surface and the bulk, with preferential segregation of POSS to the surface. Additionally, contact angle studies reveal significant reduction in surface energy and increase in hysteresis with incorporation of POSS nanoparticles. The differences in bulk and surface properties are largely explained by the gradient concentration of POSS in the polymer matrix, driven by POSS/POSS and POSS/polymer interactions.

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“…Muller et al showed that an increase in nanometerscale roughness caused a higher rate of IgG (bovine) adsorption on a well defined silicon surface. 52 Adsorption levels on the rougher surface were found to increase above what would be expected given the increase in surface area. The same effect has also been observed in studies of surfaces with nearly-random nanometer-scale roughness, as applied in this study.…”

Section: Effect Of Surface Roughnessmentioning

confidence: 65%

Factors influencing antibody stability at solid–liquid interfaces in a high shear environment

Biddlecombe¹,

Smith²,

Uddin³

et al. 2009

Biotechnology Progress

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A rotating disk shear device was used to study the effect of interfacial shear on the structural integrity of human monoclonal antibodies of IgG4 isotype. Factors associated with the solution conditions (pH, ionic strength, surfactant concentration, temperature) and the interface (surface roughness) were studied for their effect on the rate of IgG4 monomer loss under high shear conditions. The structural integrity of the IgG4 was probed after exposure to interfacial shear effects by SDS-PAGE, IEF, dynamic light scattering, and peptide mapping by LC-MS. This analysis revealed that the main denaturation pathway of IgG4 exposed to these effects was the formation of large insoluble aggregates. Soluble aggregation, breakdown in primary structure, and chemical modifications were not detected. The dominant factors found to affect the rate of IgG4 monomer loss under interfacial shear conditions were found to be pH and the nanometer-scale surface roughness associated with the solid-liquid interface. Interestingly, temperature was not found to be a significant factor in the range tested (15-45 degrees C). The addition of surfactant was found to have a significant stabilizing effect at concentrations up to 0.02% (w/v). Implications of these findings for the bioprocessing of this class of therapeutic protein are briefly discussed.

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Impact of nanometer-scale roughness on contact-angle hysteresis and globulin adsorption

Cited by 94 publications

References 73 publications

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Surface energetics, dispersion, and nanotribomechanical behavior of POSS/PP hybrid nanocomposites

Factors influencing antibody stability at solid–liquid interfaces in a high shear environment

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