2011
DOI: 10.1021/jf2011134
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Impact of Low Hydration of Barley Grain on β-Glucan Degradation and Lipid Transfer Protein (LTP1) Modifications During the Malting Process

Abstract: One of the objectives of the malting industry is to reduce the energy cost during kilning without major effect on malt quality. In this study, the impact of a low hydration steeping process on lipid transfer protein (LTP1) modifications and β-glucan breakdown was evaluated in low (LH) and high (HH) hydrated malts. LTP1 modifications analyzed by MS/MS revealed acylation, glycation, and disulfide bond breakage in both LH and HH malts. LTP1 free amine content measurement and fluorescence of Maillard protein adduc… Show more

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Cited by 9 publications
(4 citation statements)
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“…Since one of the aims of the malting industry is to reduce the energy cost during kilning, Runavot et al (2011) determined the impact of a low hydration steeping process on LTP1 modification and b-glucan breakdown. MS/MS analysis of low and high hydrated malts showed that acylation, glycation, and disulfide bond breakage occurred in both.…”
Section: Characterization Of Malt Proteins By Separation Techniques Cmentioning
confidence: 99%
“…Since one of the aims of the malting industry is to reduce the energy cost during kilning, Runavot et al (2011) determined the impact of a low hydration steeping process on LTP1 modification and b-glucan breakdown. MS/MS analysis of low and high hydrated malts showed that acylation, glycation, and disulfide bond breakage occurred in both.…”
Section: Characterization Of Malt Proteins By Separation Techniques Cmentioning
confidence: 99%
“…e surfaces and internal structures of malt may influence the rate of enzyme hydrolysis with undegraded proteins limiting the movement of enzymes in the starchy endosperm. e uneven pattern of modification results in poor-quality wort with low extract, higher viscosity, and slow wort separation (filtration) [41,42]. prediction of the viability of yeast and the efficiency of fermentation [42].…”
Section: Effect Of Steeping Time and Germination Time On Brixmentioning
confidence: 99%
“…e uneven pattern of modification results in poor-quality wort with low extract, higher viscosity, and slow wort separation (filtration) [41,42]. prediction of the viability of yeast and the efficiency of fermentation [42]. A low FAN content delays the ageing process, whilst a very high FAN content affects the sensory properties of the beer and its microbiological quality.…”
Section: Effect Of Steeping Time and Germination Time On Brixmentioning
confidence: 99%
“…However, it was found that there was degradation of the cell wall, even if only partially, since the levels of these parameters reduced over the days of germination, that is, the presence of cytolytic enzymes was confirmed during malting. Runavot (2011) explain that the low degree of maceration causes poor diffusion of β-glucanases, which in turn negatively impacts the degradation of β-glucans -reduces degradation. However, the degree of maceration reached was high: 47.29% for CC8 and 45.64% for CP8, confirming that hydrolysis was not impaired by absorption, but by poor distribution of water content within the grain, which it also affects the distribution of β-glucanases.…”
Section: Evolution Of Maltingmentioning
confidence: 99%