Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples

Matías‐García, Pamela R.; Wilson, Rory; Mussack, Veronika; Reischl, Eva; Waldenberger, Mélanie; Gieger, Christian; Anton, Gabriele; Peters, Annette; Kuehn-Steven, Andrea

doi:10.1371/journal.pone.0227648

Cited by 43 publications

(27 citation statements)

References 65 publications

(103 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In recent years, the research has been focused on liquid biopsies as a source of potential biomarkers for improving PCa diagnosis, prognosis, and patient management. The expression of miRNAs in serum, plasma, or urine is being eagerly studied for this purpose [ 19 , 20 , 21 ]. Still, there are almost no published studies on seminal plasma as a potential source of miRNA as a PCa biomarker.…”

Section: Discussionmentioning

confidence: 99%

MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia

Abramović

Vrhovec

Škara

et al. 2021

Cancers

View full text Add to dashboard Cite

Prostate cancer (PCa) is the most commonly diagnosed neoplasm among men. Since it often resembles benign prostate hyperplasia (BPH), biomarkers with a higher differential value than PSA are required. Epigenetic biomarkers in liquid biopsies, especially miRNA, could address this challenge. The absolute expression of miR-375-3p, miR-182-5p, miR-21-5p, and miR-148a-3p were quantified in blood plasma and seminal plasma of 65 PCa and 58 BPH patients by digital droplet PCR. The sensitivity and specificity of these microRNAs were determined using ROC curve analysis. The higher expression of miR-182-5p and miR-375-3p in the blood plasma of PCa patients was statistically significant as compared to BPH (p = 0.0363 and 0.0226, respectively). Their combination achieved a specificity of 90.2% for predicting positive or negative biopsy results, while PSA cut-off of 4 µg/L performed with only 1.7% specificity. In seminal plasma, miR-375-3p, miR-182-5p, and miR-21-5p showed a statistically significantly higher expression in PCa patients with PSA >10 µg/L compared to ones with PSA ≤10 µg/L. MiR-182-5p and miR-375-3p in blood plasma show higher performance than PSA in discriminating PCa from BPH. Seminal plasma requires further investigation as it represents an obvious source for PCa biomarker identification.

show abstract

Section: Discussionmentioning

confidence: 99%

MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia

Abramović

Vrhovec

Škara

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…Among them, predictive models based on miRNAs have in theory higher chances to be robust and reproducible, since miRNAs can withstand numerous freeze-thaw cycles and long-term storage before degrading [ 111 ]. Several studies have identified miR-122 as a potential diagnostic biomarker.…”

Section: Transcriptomicsmentioning

confidence: 99%

The role of omics in the pathophysiology, diagnosis and treatment of non-alcoholic fatty liver disease

2020

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) is a multifaceted metabolic disorder, whose spectrum covers clinical, histological and pathophysiological developments ranging from simple steatosis to non-alcoholic steatohepatitis (NASH) and liver fibrosis, potentially evolving into cirrhosis, hepatocellular carcinoma and liver failure. Liver biopsy remains the gold standard for diagnosing NAFLD, while there are no specific treatments. An ever-increasing number of high-throughput Omics investigations on the molecular pathobiology of NAFLD at the cellular, tissue and system levels produce comprehensive biochemical patient snapshots. In the clinical setting, these applications are considerably enhancing our efforts towards obtaining a holistic insight on NAFLD pathophysiology. Omics are also generating non-invasive diagnostic modalities for the distinct stages of NAFLD, that remain though to be validated in multiple, large, heterogenous and independent cohorts, both cross-sectionally as well as prospectively. Finally, they aid in developing novel therapies. By tracing the flow of information from genomics to epigenomics, transcriptomics, proteomics, metabolomics, lipidomics and glycomics, the chief contributions of these techniques in understanding, diagnosing and treating NAFLD are summarized herein.

show abstract

“…miRNAs are detectable by polymerase chain reaction (PCR), offering rapid and highly sensitive detection in a matter of hours. miRNAs are resistant to degradation after days at room temperature (Mall et al, 2013), after numerous freeze-thaw cycles (Matias-Garcia et al, 2020) and even when total RNA integrity is compromised (Jung et al, 2010). Together, these attributes have led to intense investigation of their utility as noninvasive diagnostic biomarkers, spurring the detection of cancer (Paranjape et al, 2009), diabetes (Jiménez-Lucena et al, 2018), cardiovascular disease (Zhou et al, 2018) and other pathologies.…”

Section: Micrornamentioning

confidence: 99%

Extracellular vesicles, microRNA and the preimplantation embryo: non-invasive clues of embryo well-being

Hawke

Watson

Betts

2021

Reproductive BioMedicine Online

View full text Add to dashboard Cite

• Extracellular vesicles and microRNA comprise the blastocyst secretome • Vesicles are elevated for blastocysts with diminished developmental competence • Vesicles may be rapidly detected by nanoparticle tracking analysis and flow cytometry • Ailing blastocysts release greater variety and amounts of microRNA • Studies have yet to show clear correlation between microRNA and embryo competence RBMO VOLUME 00 ISSUE 0 2020

show abstract

Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples

Abstract: Membership of the KORA study group is provided in the Acknowledgments.

Cited by 43 publications

References 65 publications

MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia

MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia

The role of omics in the pathophysiology, diagnosis and treatment of non-alcoholic fatty liver disease

Extracellular vesicles, microRNA and the preimplantation embryo: non-invasive clues of embryo well-being

Contact Info

Product

Resources

About