MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia

Abramović, Irena; Vrhovec, Borna; Škara, Lucija; Vrtarić, Alen; Gabaj, Nora Nikolac; Kuliš, Tomislav; Štimac, Goran; Ljiljak, Dejan; Ružić, Boris; Kaštelan, Željko; Krušlin, Božo; Bulić-Jakuš, Floriana; Ulamec, Monika; Katušić-Bojanac, Ana; Sinčić, Nino

doi:10.3390/cancers13092068

Cited by 23 publications

(16 citation statements)

References 45 publications

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“…Similar findings in cell adhesion, migration, and invasion were observed in other PCa cells studies [ 12 , 14 , 16 , 18 , 44 , 45 ] and in other tumor models [ 32 , 46 , 47 ]. Altogether, these results support the oncomiR function of miR-182-5p in regulating cell proliferation, adhesion, and migration of PCa cells, which largely determines their tumorigenic capacity and the observed unfavorable outcome in PCa clinical cases that present with miR-182-5p overexpression [ 8 , 9 , 10 , 12 , 13 , 14 , 15 , 45 ].…”

Section: Discussionsupporting

confidence: 61%

“…MiRNAs were shown to be dysregulated in PCa, among them the highly conserved miR-183 family. This family is composed of three members, miR-96, miR-182, and miR-183, mapped at 7q32.2, which up-regulation has been reported in PCa clinical cases [ 7 , 8 ]. MiR-182-5p specifically, has been shown to be overexpressed in PCa tissues [ 8 , 9 , 10 , 11 , 12 ] in association with tumor progression and decrease in survival rates [ 10 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…This family is composed of three members, miR-96, miR-182, and miR-183, mapped at 7q32.2, which up-regulation has been reported in PCa clinical cases [ 7 , 8 ]. MiR-182-5p specifically, has been shown to be overexpressed in PCa tissues [ 8 , 9 , 10 , 11 , 12 ] in association with tumor progression and decrease in survival rates [ 10 , 13 , 14 , 15 ]. In PCa cell models, deregulation of miR-182-5p has been shown to significantly impact cell proliferation, colony formation, migration, and invasion [ 12 , 14 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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MiR-182-5p Modulates Prostate Cancer Aggressive Phenotypes by Targeting EMT Associated Pathways

Souza

Cólus

Fonseca³

et al. 2022

Biomolecules

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Prostate cancer (PCa) is a clinically heterogeneous disease, where deregulation of epigenetic events, such as miRNA expression alterations, are determinants for its development and progression. MiR-182-5p, a member of the miR-183 family, when overexpressed has been associated with PCa tumor progression and decreased patients’ survival rates. In this study, we determined the regulatory role of miR-182-5p in modulating aggressive tumor phenotypes in androgen-refractory PCa cell lines (PC3 and DU-145). The transient transfection of the cell lines with miR-182-5p inhibitor and mimic systems, significantly affected cell proliferation, adhesion, migration, and the viability of the cells to the chemotherapeutic agents, docetaxel, and abiraterone. It also affected the protein expression levels of the tumor progression marker pAKT. These changes, however, were differentially observed in the cell lines studied. A comprehensive biological and functional enrichment analysis and miRNA/mRNA interaction revealed its strong involvement in the epithelial-mesenchymal transition (EMT) process; expression analysis of EMT markers in the PCa transfected cells directly or indirectly modulated the analyzed tumor phenotypes. In conclusion, miR-182-5p differentially impacts tumorigenesis in androgen-refractory PCa cells, in a compatible oncomiR mode of action by targeting EMT-associated pathways.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MiR-182-5p Modulates Prostate Cancer Aggressive Phenotypes by Targeting EMT Associated Pathways

Souza

Cólus

Fonseca³

et al. 2022

Biomolecules

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“…Prostate cancer (PC) with high incidence (>1.1 million new cases each year) and mortality (~300,000 deaths per year) presents a significant global public health burden [ 1 , 2 ]. Established risk factors of PC are age, race and family history of disease [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Prostate Cancer—Focus on Cholesterol

Škara

Turković

Pezelj

et al. 2021

Cancers

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Prostate cancer (PC) is the most common malignancy in men. Common characteristic involved in PC pathogenesis are disturbed lipid metabolism and abnormal cholesterol accumulation. Cholesterol can be further utilized for membrane or hormone synthesis while cholesterol biosynthesis intermediates are important for oncogene membrane anchoring, nucleotide synthesis and mitochondrial electron transport. Since cholesterol and its biosynthesis intermediates influence numerous cellular processes, in this review we have described cholesterol homeostasis in a normal cell. Additionally, we have illustrated how commonly deregulated signaling pathways in PC (PI3K/AKT/MTOR, MAPK, AR and p53) are linked with cholesterol homeostasis regulation.

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“…Alam et al found that MiR-375 regulates cell growth, proliferation, cell migration, and apoptosis by modulating the CTGF-EGFR-PIK3CA-AKT signaling axis in human colorectal cancer cells (18) . Furthermore, several investigations were demonstrated that constitutive expression of miR-375 promotes oncogenicity in the breast (19)(20)(21)(22)(23) , prostate (24)(25)(26)(27) , and lung cancer (28) . In addition, few pieces of literature reported that miR-375 functions as a tumor suppressor (16,(29)(30)(31) .…”

Section: Introductionmentioning

confidence: 99%

Knockdown of microRNA-375 suppresses cell proliferation and promotes apoptosis in human breast cancer cells

Moorthy¹,

Srinivasan²,

Jayamohan³

et al. 2021

IJST

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Background:The purpose of the present investigation is to unravel the influence of the miR-375 inhibitor on cell survival descent and apoptosis stimulation, mediating PI3K/Akt/mTOR signaling network in human breast cancer cells. Methods: MCF-7 cells were transfected with miR-375 inhibitor for 72 h. Then, cell survival assay was measured by MTT. Cells were stained with EtBr/AO, DAPI, and DCFH-DA to assess the effect of the miR-375 inhibitor on cell death. A scratch experiment was performed to observe the cell migration ability. The expression of anti-apoptotic and apoptotic genes such as BCL-2, BAX, and PI3K/Akt/mTOR and miR-375 were evaluated in miR-375 inhibitor transfected cells by qRT-PCR. Findings: MiR-375 inhibitor sensitized tumor cells and influenced significant loss in the breast cancer cell proliferation with obvious cell death elevation. MiR-375 inhibitor effectively augmented ROS generation. Also, miR-375 inhibition hampered migratory ability. Furthermore, our qRT-PCR analysis showed that inhibition of the miR-375 was able to significantly reduce the constitutive expression of PI3K/Akt/mTOR mRNAs. Additionally, miR-375 suppression decreased the anti-apoptotic gene, Bcl-2 expression and enhanced pro-apoptotic gene, Bax expression along with potentially decreasing miR-375 level compared to control. Novelty and applications: Inhibition of the miR-375 has considerably attenuated cell proliferation and stimulated apoptotic cell death in the breast cancer cells. Thus, miR-375 represent a potential therapeutic target for the breast cancer.

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MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia

Cited by 23 publications

References 45 publications

MiR-182-5p Modulates Prostate Cancer Aggressive Phenotypes by Targeting EMT Associated Pathways

MiR-182-5p Modulates Prostate Cancer Aggressive Phenotypes by Targeting EMT Associated Pathways

Prostate Cancer—Focus on Cholesterol

Knockdown of microRNA-375 suppresses cell proliferation and promotes apoptosis in human breast cancer cells

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