Impact of light and sucrose supplementation on cellular differentiation, metabolic shift and modulation of gene expression in hairy roots of Daucus carota

Mukherjee, Subhadip; Kutty, Nithya N.; Bera, Paramita; Mitra, Adinpunya

doi:10.1007/s11240-018-1523-5

Cited by 16 publications

(7 citation statements)

References 40 publications

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“…Although various types of carbon resources have been applied for in vitro culture of tree species, sucrose is still a reliable carbon resource applied in Eucalyptus [152]. Sucrose improved the productivity of secondary metabolites and led to cellular differentiation, metabolic shift, and modulation of gene expression [153][154][155]. Comparing with reducing sugars, sucrose as non-reducing sugar is less active and able to translocate more [119,140].…”

Section: Effect Of Carbohydratementioning

confidence: 99%

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Abiri

Atabaki

Hamid

et al. 2020

Forests

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Micropropagation is a reliable technique in biotechnology and genetic engineering domain, which has been widely applied for rapid mass propagation of plants in vitro condition. Through micropropagation techniques, reproduction of plants can be attained from different explants using organogenesis and somatic embryogenesis. Over the decades, micropropagation techniques have offered tremendous potential for forest tree improvement. Eucalyptus is a woody plant species recalcitrant to in vitro culture. In general, the micropropagation of Eucalyptus culture processes and the genotype, environment surroundings, and age of explants in culture media is frequently linked with the occurrence of micropropagation variation. In the current review paper, an update of the most important physiological and molecular phenomena aspects of Eucalyptus micropropagation was linked to the most profound information. To achieve the mentioned target, the effect of plant growth regulators (PGRs), nutrients, other adjuvant and environmental features, as well as genetic interaction with morpho- and physiological mechanisms was studied from the induction to plant acclimatisation. On the other hand, important mechanisms behind the organogenesis and somatic embryogenesis of Eucalyptus are discussed. The information of current review paper will help researchers in choosing the optimum condition based on the scenario behind the tissue culture technique of Eucalyptus. However, more studies are required to identify and overcome some of the crucial bottlenecks in this economically important forest species to establish efficient micropropagation protocol at the industrial level.

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Section: Effect Of Carbohydratementioning

confidence: 99%

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Abiri

Atabaki

Hamid

et al. 2020

Forests

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“…The lighting effect is very important for the correct development of plant cells and tissues (MUKHERJEE et al, 2019). In all surviving seedling treatments of this work (Figure 2), there were one layer of adaxial epidermis, one layer of palisade parenchyma perpendicular to the epidermis, three to four layers of spongy parenchyma with irregularly shaped cells, bicolateral bicuspid vascular bundles and one layer of abaxial epidermis.…”

Section: Sucrose Effect On Physalis Angulata Micropropagationmentioning

confidence: 77%

“…From the leaf anatomical point of view, sucrose decreased the leaf thickness, probably due to the reduction of the palisade and spongy parenchyma ( Table 2). The effect of sucrose is very important for the correct development of plant cells and tissues (MUKHERJEE et al, 2019). It was observed that the 0 Sucrose treatment had a greater thickness of palisade parenchyma, spongy and foliar thickness in relation to the other treatments (Table 2).…”

Section: Sucrose Effect On Physalis Angulata Micropropagationmentioning

confidence: 97%

Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata

et al. 2020

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The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose.

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“…The concentrations of between 3%-5% sucrose have frequently been reported as optimal for biomass accumulation and production of secondary metabolites from adventitious roots and hairy root cultures, whereas higher concentrations of sucrose have an inhibitory effect on plant tissue culture growth (Vazquez-Flota et al, 1994;Baque et al, 2012;Praveen & Murthy, 2012). Similarly, the supplementation of cultures with sucrose has also been reported to influence the growth and development of hairy root cultures of Psoralea corylifolia (Shinde, Malpathak & Fulzele, 2010), Hypericum perforatum (Cui et al, 2010, Azadirachta indica (Srivastava & Srivastava, 2012) and Daucus carota (Mukherjee et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Methyl jasmonate enhances ursolic, oleanolic and rosmarinic acid production and sucrose induced biomass accumulation, in hairy roots of Lepechinia caulescens

Vergara-Martínez

Estrada‐Soto

Valencia-Díaz

et al. 2021

PeerJ

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Background Ursolic (UA), oleanolic (OA) and rosmarinic (RA) acids are bioactive metabolites found in Lepechinia caulescens that have generated interest for their health benefits, which include antimicrobial, antioxidant, antimutagenic, gastroprotective, antidiabetic, antihypertensive and anti-inflammatory properties, among others. To date, very few attempts have been made to evaluate the potential for simultaneous production of these bioactive compounds, using a biotechnological approach. Hairy root cultures offer a biotechnology approach that can be used to study the factors affecting the biosynthesis and the production of UA, OA and RA. In the current study, we established hairy root cultures of L. caulescens and evaluated the effect of sucrose on biomass accumulation, and the effect of different concentrations and times of exposure of methyl jasmonate (MeJA), on the accumulation of UA, OA and RA. Methods Leaves from plants of L. caulescens were inoculated with Agrobacterium rhizogenes strain ATCC 15834. PCR of rolB gene confirmed the transgenic nature of hairy roots. Hairy roots were subcultured in semisolid MSB5 medium, supplemented with 15, 30, 45 or 60 g/L sucrose and after 4 weeks, dry weight was determined. The accumulation of UA, OA and RA of wild plants and hairy roots were determined by HPLC. Finally, the hairy roots were treated with 0, 100, 200 and 300 µM of MeJA and the content of bioactive compounds was analyzed, after 24, 48 and 72 h. Results High frequency transformation (75%) was achieved, using leaf explants from axenic seedlings, infected with A. rhizogenes. The hairy roots showed an enhanced linear biomass accumulation, in response to the increase in sucrose concentration. The hairy root cultures in MSB5 medium, supplemented with 45 g/L sucrose, were capable to synthesizing UA (0.29 ± 0.00 mg/g DW), OA (0.57 ± 0.00 mg/g DW) and RA (41.66 ± 0.31 mg/g DW), about two, seven and three times more, respectively, than in roots from wild plants. Elicitation time and concentration of MeJA resulted in significant enhancement in the production of UA, OA and RA, with treatments elicited for 24 h, with a concentration of 300 µM of MeJA, exhibiting greatest accumulation. Conclusion This is the first report on development of hairy root cultures of L. caulescens. Future studies should aim towards further improving triterpenes and polyphenolic compound production in hairy roots of L. caulescens, for use in the pharmaceutical and biotechnological industry.

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Impact of light and sucrose supplementation on cellular differentiation, metabolic shift and modulation of gene expression in hairy roots of Daucus carota

Cited by 16 publications

References 40 publications

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata

Methyl jasmonate enhances ursolic, oleanolic and rosmarinic acid production and sucrose induced biomass accumulation, in hairy roots of Lepechinia caulescens

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