Impact of Leptin Gene Polymorphisms on Milk Production Traits in Barki Goats.

Abstract

The objective of this study was to test the association of variation in a 356 bp region in exon 1 of the caprine leptin gene with milk production traits (milk yield (MY), milk fat content (FAT), milk protein content (PRO), milk lactose content (LAC), milk solid not fat content (SNF), pH and somatic cell count (SCC)) in 106 Barki does using polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP). General linear model was used to test association between the variation in leptin and milk-production traits. The SSCP banding patterns for leptin revealed two variants (L1 and L2), which contained two nucleotide sequence difference (c.670528A/G and c.670639T/C). The c. 670528A/G substitution results in the substitution of valine with alanine and c.670639T/C results in the substitution of arginine with histidine. Association analysis between the variation in leptin with milk production traits revealed that leptin genotype was associated (P < 0.05) with PRO and highly (P < 0.001) associated with MY. The presence of the L1 variant in the doe genotype was significantly associated with lower MY (P < 0.01) and decreased PRO (P < 0.01), whereas, the presence of the L2 variant in the doe genotype was significantly associated with higher MY (P < 0.05). The detected leptin variants showed significant (P < 0.05) additive effect on pH and highly significant (P < 0.01) dominance effects on MY and PRO. This suggests that selection for the leptin genotypes might increase milk production and milk protein content in Barki goats.

Effect of Different Levels of Aflatoxin B1 in Concentrated Livestock Diets on Some Characteristics of Rumen Fluid in Laboratory with Sodium Bentonite

“…Some research suggests that various fungi accompany yellow maize crops, including those producing aflatoxin B1 [11,12].Numerous studies have focused on the destruction and removal of fungal toxins from food and forage, utilizing various chemical, physical, and biological methods [13].One of the major challenges facing livestock breeders is the lack of availability and high cost of feed, particularly during drought seasons [14,15].utrient utilization and decrease the risk of metabolic diseases [16]. These feed additives could be nutritional, like plant oils, or non-nutritional [17]. One such non-nutritional feed additive is bentonite, which possesses adsorptive properties and improves rumen fermentation.…”

Determination of serum Leptin levels in cattle, sheep, goats and buffaloes in Burdur province in Türkiye by ELISA method

“…In a study in Turkey, Guzel et al (2012) determined serum leptin levels in Brown Swiss (2.35±0.34 ng/ml), Holstein bulls (3.55±0.53 ng/ml), Avesi sheep (2.16±0.29 ng/ml), saanen (2.23±0.46 ng/ml) and hair goats (0.94±0.10ng/ml) by the radio immune assay (RIA) method. In the early 2000s, studies investigating leptin levels serologically were more common, but in the following years more genetic studies began to gain importance (Ferchichi et al, 2018;Kaplan, 2018;Abbas et al, 2019;Avondo et al, 2019;Das et al,2019;Ibrahim et al, 2020;Mahrous et al, 2020;Sedykh et al, 2020).…”