Colloidal gold nanoparticles are prepared by laser ablation method and characterized by UV-Visible spectrometry and Transmission Electron Microscopy. Fluorescence quenching of Rhodamine 6G dye with different concentration, from 0.05 to 15 lM, in the presence of gold nanoparticles have been investigated. The optical absorption and fluorescence emission of samples are studied. Shift of the fluorescence peaks in the presence of gold nanoparticles are the same as the absorption peaks for different concentrations. The stokes shift of Rhodamine 6G in different concentrations with and without gold nanoparticles is constant. Experimental quantum yield are calculated. Due to the local field enhancement by gold nanoparticles, the absorbed power of the samples in presence of nanoparticles are increased and the fluorescence intensity and fluorescence quantum yield are decreased. Also, the energy transfer efficiency is measured. Experimental results showed that at dye concentration range of our studies the presence of gold nanoparticles in the mixture results in fluorescence quenching.