Impact of interfacial cholesterol-anchored polyethylene glycol on sterol-rich non-phospholipid liposomes

Cui, Zhong‐Kai; Edwards, Katarina; Orellana, Alejandro Nieto; Bastiat, Guillaume; Benoit, Jean‐Pierre; Lafleur, Michel

doi:10.1016/j.jcis.2014.04.031

Cited by 8 publications

(6 citation statements)

References 57 publications

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“…The polyethylene glycol (PEG) caps the liposome, which gives it a longer circulation time in the blood, meaning that it decreases the uptake of the liposomes by the reticulum endothelium system (RES) and allows the drug to be released gradually [32]. In addition, PEG is biocompatible, which enables the possibility of further functionalization of the liposomes by attaching antibodies or ligands [33,34]. The 31 P spectra analysis of PEGylated liposomes obtained using the thin film method revealed information about the amount of free lipids or building micelles (narrow line) and lipids building MLV (broad shoulder) [31,35].…”

Section: Lipid Membrane Dynamic Studiesmentioning

confidence: 99%

“…The release rate of the drug depends on the rate of change in temperature and by the serum compounds (i.e., lipoproteins) [56]. Thus, as drug carriers, the liposomes should be stable in the serum and release the drug slowly at a proper temperature [34,57]. The 1 H spectra of PC and PC/octadecylamine (positively charged)…”

Section: Drug Delivery Studymentioning

confidence: 99%

“…As mentioned before, the controlled release of drugs is very important. The PA/Ch/PEG-Ch (palmitic acid/ cholesterol/PEGylated cholesterol) liposomes exhibit no permeability of drugs (calcein and doxorubicin) up to 20 mol% PEGylated cholesterol concentration, but in 10 mol% PEG-Ch concentration the permeability of membrane limited and can be controlled [34]. Moreover, the PA/Ch/PEG-Ch liposomes are stable in various pHs [34].…”

Section: Drug Delivery Studymentioning

confidence: 99%

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Dynamics of Model Membranes by NMR

Timoszyk¹

2017

Spectroscopic Analyses - Developments and Applications

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Amphiphilic molecules can create various aggregates in water. Concern about exploring such structures has been unabated for several decades due to the wide range of possible applications of lipid aggregates, from food technology to the pharmaceutical industry. The form of self-assembled structures depends on many factors, such as the type of amphiphilic molecule, the concentration, the level of hydration, the temperature, and the pH. Liposomes and micelles are the most widely known types of closed structures. Liposomes are more often used in the fields of medicine and pharmacy because they consist of nontoxic compounds and their composition and size can be controlled. Nuclear magnetic resonance (NMR) is one of the methods, which is most commonly used to study liposome properties. It can be used to observe changes in the structure, dynamics, and phase transition of lipid membranes. The membrane properties are changed under the influence of external factors, such as temperature, pH, and the presence of ions or drugs. The chapter aims to introduce and discuss the possibilities of the most useful NMR methods, 31 P and 1 H, to study the liposome properties. It also aims to show how various changes in the structure or dynamics of lipid molecules are visible in the NMR spectra.

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Section: Lipid Membrane Dynamic Studiesmentioning

confidence: 99%

Section: Drug Delivery Studymentioning

confidence: 99%

Section: Drug Delivery Studymentioning

confidence: 99%

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Dynamics of Model Membranes by NMR

Timoszyk¹

2017

Spectroscopic Analyses - Developments and Applications

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“…The most significant increase in incorporated drug was observed following the inclusion of 25 mol% cholesterol (86% drug loaded). The percent cholesterol is greater than that typically used to prepare conventional liposomes (Raffy & Teissié, 1999;Ohvo-Rekila et al, 2002;Cui et al, 2014). Approximately 44% of drug was incorporated in CLENs consisting of 50 mol% cholesterol, the lowest percent of drug incorporated compared to others evaluated (Table 3; 25 > 10 > 0 > 50 mol%).…”

Section: Discussionmentioning

confidence: 83%

Nano-formulations composed of cell membrane-specific cellular lipid extracts derived from target cells: physicochemical characterization and in vitro evaluation using cellular models of breast carcinoma

Alharbi

Campbell

2018

AAPS Open

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Highly selective drug targeting is an important goal in the development of cancer nanotechnologies. In an effort to improve tumor targeting a method was developed to formulate cell membrane lipid-extracted nanoliposomes (CLENs). The main ingredients were extracted directly from the membrane of cancer cells. For this study we used three different breast cancer cell lines (4 T1, BT-20, and SK-BR-3). As controls for the normal breast and cancer tissue environments we employed the normal breast fibroblast (CRL-2089) and ovarian cancer (SK-OV-3) cell lines, respectively. We evaluated physicochemical properties, efficiency of drug loading, cellular uptake, and cytotoxicity. The mean diameter and zeta potential values for the 5 different CLENs were 202 ± 38 nm and − 15 ± 3.8 mv, respectively. Doxorubicin hydrochloride (5 mol%) increased the size of 4 T1-CLENs from 158 ± 2 nm to 212 ± 59 nm, with no significant change in the negativelycharged surface potential. Percent of drug loaded ranged from 40 to 93%, varying according to the ratio of lipid extract to conventional components employed. The additional inclusion of cholesterol and DPPE-PEG 5000 increased drug loading in CLENs, similar to Doxil preparations. The most promising cellular uptake and cytotoxicity profiles were observed when the lipid ingredients were derived from the eventual target cell. Given the ability of CLENs to better recognize target cells compared to nanosystems consisting of non-specific lipid extracts or conventional liposome ingredients alone, CLENs has demonstrated early promise as a nano-delivery system for cancer treatment.

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“…Additionally, its fluorescence served better visualization. The liposome formulations composed of phosphatidylcholine (PC)—P1, liposome formulations composed of PC and cholesterol (30%)—P2—and liposome formulations composed of PC, cholesterol (30%), and polymer polyethylene glycol (PEG)—P3, were assessed as the most common, currently studied formulations, where cholesterol is responsible for sealing the LUVs, while PEG increases the time the LUVs circulate in the body [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Liposomal Drug Formulations on the RBCs Shape, Transmembrane Potential, and Mechanical Properties

Cyboran-Mikołajczyk

Sareło

Pasławski

et al. 2021

IJMS

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Liposomal technologies are used in order to improve the effectiveness of current therapies or to reduce their negative side effects. However, the liposome–erythrocyte interaction during the intravenous administration of liposomal drug formulations may result in changes within the red blood cells (RBCs). In this study, it was shown that phosphatidylcholine-composed liposomal formulations of Photolon, used as a drug model, significantly influences the transmembrane potential, stiffness, as well as the shape of RBCs. These changes caused decreasing the number of stomatocytes and irregular shapes proportion within the cells exposed to liposomes. Thus, the reduction of anisocytosis was observed. Therefore, some nanodrugs in phosphatidylcholine liposomal formulation may have a beneficial effect on the survival time of erythrocytes.

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Impact of interfacial cholesterol-anchored polyethylene glycol on sterol-rich non-phospholipid liposomes

Abstract: Table of content (TOC) Graphic Highlights• PEGylated liposomes strictly made from palmitic acid and cholesterol are created

Cited by 8 publications

References 57 publications

Dynamics of Model Membranes by NMR

Dynamics of Model Membranes by NMR

Nano-formulations composed of cell membrane-specific cellular lipid extracts derived from target cells: physicochemical characterization and in vitro evaluation using cellular models of breast carcinoma

Impact of Liposomal Drug Formulations on the RBCs Shape, Transmembrane Potential, and Mechanical Properties

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