Impact of initial explants on in vitro propagation of native potato (Solanum tuberosum, Andigena group)

Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures

“…Similar to our results, Mestinšek-Mubi et al [ 34 ] showed that basal-derived nodal explants resulted in maximum shoot length and node numbers, which were significantly greater than apical-derived explants in two drug-type cannabis genotypes (MX-CBD-11 and MX-CBD-707). Comparable results were also reported with other plant species such as Salvia tomentosa [ 21 ], Rauvolfia serpentina [ 35 ], Quercus robur [ 36 ], Persea americana [ 37 ], Solanum tuberosum [ 38 ], Ginkgo biloba [ 20 ], Rubus fruticosus [ 39 ], Caralluma lasiantha [ 40 ], Psidium guajava [ 41 ], Hemidesmus indicus [ 42 ], Ficus religiosa [ 43 ], and Cedrela montana [ 44 ] which showed that explants derived from basal parts had better performance than those apical-derived explants. The endogenous balance of phytohormones in the explants obtained from the basal and near-basal parts of the mother plant contributes to the emergence of their juvenile characteristics [ 35 , 45 ].…”

“…The endogenous balance of phytohormones in the explants obtained from the basal and near-basal parts of the mother plant contributes to the emergence of their juvenile characteristics [ 35 , 45 ]. Therefore, such explants have been reported as the best source of initial explants for in vitro rejuvenation practices [ 21 , 38 , 40 , 44 ]. Moreover, such explants have better performance in tackling the adverse effects of somaclonal variations [ 23 , 46 , 47 ].…”

“…Moreover, leaf morphology analysis showed that the explants derived from the basal part of the mother plant were better suited to be initial explants for in vitro cannabis rejuvenation practices. Since previous studies on other plant species, such as potatoes [ 38 ], showed that the effects of explant source on in vitro multiplication and somaclonal variation are genotype dependent, further investigations should be evaluated using different cannabis cultivars. Although this study demonstrates the importance of explant source on initial and long-term health of cannabis cultures, further studies with greater replications are needed.…”