Impact of Saussurea lappa root extract against copper oxide nanoparticles induced oxidative stress and toxicity in rat cardiac tissues

Abstract

Copper oxide nanoparticles (CuO NPs) have developed as a significant class of nanomaterial with potential dangers to organisms and the environment in a variety of applications. This study aimed to investigate the impact of costus root extract against CuO NPs induced oxidative stress, alterations in heart structure and functions. 40 adult male rats were assigned randomly to four groups: first; control, second; costus (300 mg/kg body weight/day) orally for 2 weeks, third; CuO NPs (100 mg/kg body weight/day) intraperitoneally for 4 weeks and fourth; CuO NPs + costus.Current results revealed, significant increases in serum levels of creatine kinase-MB, creatine kinase enzyme, lactate dehydrogenase, myoglobin, aspartate aminotransferase, alkaline phosphatase, cardiac TBIRS, total thiol, nitric oxide, and cardiac proliferating cell nuclear antigen after CuO NPs administration when compared with control group. Conversely, statistical significant decreases were detected in cardiac reduced glutathione, catalase, and superoxide dismutase in CuO NPs group as compared with control group. Interestingly, treatment of CuO NPs with costus root extract was associated with significant improvements of the studied parameters, heart structure and functions. CuO NPs-induced toxicity, injury and oxidative stress in rat heart and treatment with Costus root extract could scavenge free radicals producing beneficial effects against CuO NPs.

Quercetin and l-Arginine Ameliorated the Deleterious Effects of Copper Oxide Nanoparticles on the Liver of Mice Through Anti-inflammatory and Anti-apoptotic Pathways

“…The primary mechanism of CuO NP toxicity is attributed to the overproduction of reactive oxygen species (ROS), progression of oxidative stress, inflammation, biomolecule destruction, and genotoxicity. These effects occur when CuO NPs contact with the cell membrane and pass via tiny endocytic vesicle [ 32 – 34 ]. Excessive production of ROS is critical in the induction and progression of several diseases including liver disease [ 35 ].…”

Ehrlich ascites carcinoma provokes renal toxicity and DNA injury in mice: Therapeutic impact of chitosan and maitake nanoparticles

“…Formalin‐preserved kidney tissues were embedded in paraffin after being treated with serial concentrations of ethanol and methyl benzoate. The 5‐μm‐thick sections were performed before staining with eosin and haematoxylin 36 . For immunohistochemistry, the samples dewaxed and rehydrated following the standard methods 37 .…”

“…The 5-μm-thick sections were performed before staining with eosin and haematoxylin. 36 For immunohistochemistry, the samples dewaxed and rehydrated following the standard methods. 37 In nutshell, the sections were incubated with the following antibodies gained from Dako; Agilent: anti-P53…”

Copper and Zinc Particles as Regulators of Cardiovascular System Function—A Review

“…The increase in cardiac enzymes could be attributed to the CuO-NPs' emission of free radicals. CuO-NPs induced severe cardiac injury, characterized by myocardial hypertrophy and severe focal necrosis of cardiac myocytes accompanied by an infiltration of inflammatory cells [51]. According to Cholewi ńska et al the substitution of copper carbonate (ionic form of copper) with metal nanoparticles in the food consumed by rats could worsen the adverse effects caused by hypertension on the heart, liver, and intestines [48].…”