Impact of <i>Phakopsora pachyrhizi</i> Infection on Soybean Leaf Photosynthesis and Radiation Absorption

Kumudini, S.; Prior, E.; Omielan, J.; Tollenaar, M.

doi:10.2135/cropsci2008.05.0258

Cited by 27 publications

(27 citation statements)

References 14 publications

(3 reference statements)

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“…In cucumber leaves infected by Sphaerotheca fuliginea, for example, impairments to A were associated with reductions in Chl concentrations, damage to the thylakoids, and the loss of chloroplast stroma (1). In soybean leaves infected by Phakopsora pachyrhizi, impairments to A were due mainly to lower Chl concentrations and the reduced capacity of the leaves to absorb light (23).…”

Section: Discussionmentioning

confidence: 99%

Leaf Gas Exchange and Chlorophyll a Fluorescence Imaging of Rice Leaves Infected with Monographella albescens

Tatagiba¹,

DaMatta²,

Rodrigues³

2015

Phytopathology®

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This study was intended to analyze the photosynthetic performance of rice leaf blades infected with Monographella albescens by combining chlorophyll (Chl) a fluorescence images with gas exchange and photosynthetic pigment pools. The net CO2 assimilation rate, stomatal conductance, transpiration rate, total Chl and carotenoid pools, and Chl a/b ratio all decreased but the internal CO2 concentration increased in the inoculated plants compared with their noninoculated counterparts. The first detectable changes in the images of Chl a fluorescence from the leaves of inoculated plants were already evident at 24 h after inoculation (hai) and increased dramatically as the leaf scald lesions expanded. However, these changes were negligible for the photosystem II photochemical efficiency (Fv/Fm) at 24 hai, in contrast to other Chl fluorescence traits such as the photochemical quenching coefficient, yield of photochemistry, and yield for dissipation by downregulation; which, therefore, were much more sensitive than the Fv/Fm ratio in assessing the early stages of fungal infection. It was also demonstrated that M. albescens was able to impair the photosynthetic process in both symptomatic and asymptomatic leaf areas. Overall, it was proven that Chl a fluorescence imaging is an excellent tool to describe the loss of functionality of the photosynthetic apparatus occurring in rice leaves upon infection by M. albescens.

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Section: Discussionmentioning

confidence: 99%

Leaf Gas Exchange and Chlorophyll a Fluorescence Imaging of Rice Leaves Infected with Monographella albescens

Tatagiba¹,

DaMatta²,

Rodrigues³

2015

Phytopathology®

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“…As P. pachyrhizi infection progresses, reduced photosynthesis and assimilation of CO 2 occur, due in part to defoliation and reduction of the green leaf area index as lesions form (Kumudini et al, 2008a). In addition, P. pachyrhizi also reduces the efficiency of photosynthesis in the remaining green tissues that visually appear to be healthy (Kumudini et al, 2008b). This suggests that the down-regulation of photosynthesis observed by Tremblay et al (2010) is a consequence of disease and not a resistance response.…”

Section: Nature Of the Differentially Expressed Genesmentioning

confidence: 99%

Biphasic Gene Expression Changes Elicited byPhakopsora pachyrhiziin Soybean Correlate with Fungal Penetration and Haustoria Formation

et al. 2011

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Inoculation of soybean (Glycine max) plants with Phakopsora pachyrhizi, the causal organism of Asian soybean rust, elicits a biphasic response characterized by a burst of differential gene expression in the first 12 h. A quiescent period occurs from 24 to 48 h after inoculation, in which P. pachyrhizi continues to develop but does not elicit strong host responses, followed by a second phase of intense gene expression. To correlate soybean responses with P. pachyrhizi growth and development, we inoculated the soybean cultivar Ankur (accession PI462312), which carries the Rpp3 resistance gene, with avirulent and virulent isolates of P. pachyrhizi. The avirulent isolate Hawaii 94-1 elicits hypersensitive cell death that limits fungal growth on Ankur and results in an incompatible response, while the virulent isolate Taiwan 80-2 grows extensively, sporulates profusely, and produces a compatible reaction. Inoculated leaves were collected over a 288-h time course for microarray analysis of soybean gene expression and microscopic analysis of P. pachyrhizi growth and development. The first burst in gene expression correlated with appressorium formation and penetration of epidermal cells, while the second burst of gene expression changes followed the onset of haustoria formation in both compatible and incompatible interactions. The proliferation of haustoria coincided with the inhibition of P. pachyrhizi growth in the incompatible interaction or the beginning of accelerated growth in the compatible interaction. The temporal relationships between P. pachyrhizi growth and host responses provide an important context in which to view interacting gene networks that mediate the outcomes of their interactions.

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“…The photosynthesis system's chamber (LI6400-40) has a sampling area of 2 cm 2 and an internal red-blue LED light source that was used to obtain the desired photosynthetic photo flux density (PPFD) and take chlorophyll fluorescence measurements (Kumudini et al 2008). For measurement of electron transport rate (ETR), the modulation frequency of the measuring light was 10 kHz under actinic illumination and increased to 20 kHz during saturating pulses.…”

Section: Photosynthesis Analysismentioning

confidence: 99%

“…where f is the fraction of absorbed quanta that is used by photosystem 2 (PS2) and is typically assumed to be 0.5 for C3 plants (Kumudini et al 2008). …”

Section: Photosynthesis Analysismentioning

confidence: 99%

Glyphosate affects photosynthesis in first and second generation of glyphosate-resistant soybeans

et al. 2010

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The crop area planted to conventional soybeans has decreased annually while that planted to glyphosate-resistant (RR) soybean has drastically increased mainly due to the wide adoption of glyphosate in current weed management systems. With the extensive use of glyphosate, many farmers have noted visual plant injury in RR soybean varieties after glyphosate application. A new generation designated as "second generation-RR2" has been recently developed and these RR2 cultivars already are commercially available for farmers and promoted as higher yielding relative to the previous RR cultivars. However, little information is currently available about the performance of RR2 soybean beyond commercial and farmer testimonial data. Thus, an evaluation of different glyphosate rates applied in different growth stages of the first and second generation of RR soybeans, revealed a significant decrease in photosynthesis. In general, increased glyphosate rate and late applications (V6) pronounced decrease photosynthetic parameters and consequently decreased in leaf area and shoot biomass production. In contrast, low rate and early applications were less damage for the RR soybean plants, suggesting that with early applications (V2), plants probably have more time to recover from glyphosate or its metabolites effects regarding late applications.

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Impact of Phakopsora pachyrhizi Infection on Soybean Leaf Photosynthesis and Radiation Absorption

Cited by 27 publications

References 14 publications

Leaf Gas Exchange and Chlorophyll a Fluorescence Imaging of Rice Leaves Infected with Monographella albescens

Leaf Gas Exchange and Chlorophyll a Fluorescence Imaging of Rice Leaves Infected with Monographella albescens

Biphasic Gene Expression Changes Elicited byPhakopsora pachyrhiziin Soybean Correlate with Fungal Penetration and Haustoria Formation

Glyphosate affects photosynthesis in first and second generation of glyphosate-resistant soybeans

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