Impact of Croton argyrophyllus essential oil on behavioural models of nociception

Abstract

Croton argyrophyllus Kunth (Euphorbiaceae) is a shrub abundant in the northeast region of Brazil where it possesses ethnobotanical use. To assess the antinociceptive action of C. argyrophyllus, studies were conducted in behavioural models of nociception, and some of the mechanisms given under this effect investigated. The essential oil (EO) of C. argyrophyllus leaves (10, 30 and 100 mg/kg) was used in mice via the oral route in both the chemicals (acetic acid, formalin, capsaicin and glutamate) and thermal nociception models. The EO produced inhibition of acetic acid‐induced visceral pain (10, 30 and 100 mg/kg, p.o.) and it produced a significant antinociception effect in the hot plate test (100 mg/kg). In the formalin test, the EO caused significant inhibition of both the early (100 mg/kg) and the late (30 and 100 mg/kg) phases of formalin‐induced licking. The EO (30 and 100 mg/kg) also caused significant inhibition of capsaicin‐ and glutamate‐induced pain. The EO (100 mg/kg) antinociception in the hot plate test was significantly attenuated by treatment with naloxone. In contrast, the EO (100 mg/kg) antinociception in the abdominal constriction test was not affected by treatment of mice with L‐arginine. The present results suggest that the EO from C. argyrophyllus produced antinociception in several models of pain in mechanisms that involved glutamatergic and opioid systems. Copyright © 2016 John Wiley & Sons, Ltd.

Effect of the Croton rhamnifolioides Essential Oil and the Inclusion Complex (OEFC/β-CD) in Antinociceptive Animal Models

“…In a previous study conducted with the Croton rhamnifolioides species, this was shown to possess gastroprotective effects involving the opioid system and the nitric oxide pathway [5], which are results that corroborate with our study. Some studies investigating the possible mechanisms of action involved in the antinociceptive response with species from the Croton genus, such as those using the Croton conduplicatus [57] and Croton argyrophyllus Kunth [58] essential oils, also support our research. Moreover, a previous study described the anti-inflammatory effect of the OEFC [3] and COEFC [16], which are results that corroborate with the antinociceptive action observed in the present study, since pain is a classic sign of the inflammatory process.…”

FENÓLICOS TOTAIS, FLAVONOIDES TOTAIS E ATIVIDADE ANTIOXIDANTE DE EXTRATOS DE Croton argyrophyllus KUNTH (EUPHORBIACEAE)

“…Estudos realizados já demonstraram que C. argyrophyllus produz óleo essencial, apresentando algumas atividades biológicas, como a atividade inseticida sobre larvas e adultos de Aedes Aegypti (CRUZ et al, 2017), o efeito antiinflamatório e antioxidante (RAMOS et al, 2013), o efeito antinociceptivo (RAMOS et al, 2016) e a atividade citotóxica (ARAÚJO et al, 2014). Entretanto, estudos relacionados com a atividade antioxidante e os teores de fenólicos e flavonoides totais dos extratos vegetais ainda não foram encontrados.…”

Application crude multienzyme extract from Aspergillus niger as a pretreatment for the extraction of essential oil from Croton argyrophyllus leaves

“…Generally, the major constituents are responsible for the biological activities of essential oils extracted from different aromatic species. 13,55 In this context, the biological activities of C. argyrophyllus essential oil reported by TA B L E 4 Chemical composition of C. argyrophyllus essential oil obtained by hydrodistillation using the following samples: Fresh leaves without enzymatic pretreatment (FL 0 ), fresh leaves with enzymatic pretreatment (FL ept ), and dried leaves without enzymatic pretreatment (DL) several authors [11][12][13][14] may be attributed to high concentrations of bicyclogermacrene, β-caryophyllene, spathulenol, and α-pinene, the major constituents in those studies and in the present one.…”

“…The C. argyrophyllus essential oil has promising active components for the development of drugs and botanical insecticides. [11][12][13][14] Therefore, the search for new methodologies capable of maximizing the extraction yield of these…”

“…Croton argyrophyllus Kunth is a shrub species normally found in countries of South America; its essential oil has a toxic effect against Aedes aegypti larvae and adults 11 as well as anti-inflammatory and antioxidant activities, 12 antinociceptive effect, 13 and cytotoxic activity. 14 There are different methods for extracting essential oils from aromatic plants.…”