Impact of hypofunctional occlusion on upper and lower molars after cessation of root development in adult mice

Chandrasekaran, Dhivya; Milétich, Isabelle; Tucker, Abigail S.

doi:10.1093/ejo/cjw051

Cited by 3 publications

(3 citation statements)

References 27 publications

(17 reference statements)

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“…Following occlusal unloading, macroscopic teeth overeruption in the HG could be attributed to two main reasons: (1) the elongation of teeth themselves 30,31 and (2) the increase in alveolar bone height, 32,33 which were both observed in our research. Apart from these, a gain in the mandibular width in the HG was an unexpected discovery, in disagreement with the conventional view that occlusal force would thicken the bone as a result of adaptive bone remodeling 34–36 .…”

Section: Discussionsupporting

confidence: 65%

Periodontal ligament‐associated protein‐1 engages in teeth overeruption and periodontal fiber disorder following occlusal hypofunction

Chen

Luo

Xie

et al. 2022

J of Periodontal Research

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Background and Objective: Teeth overeruption is a problem of clinical significance, but the underlying mechanism how changes in external occlusal force convert to the periodontium remodeling signals has been a largely under explored domain. And recently, periodontal ligament-associated protein-1 (PLAP-1)/asporin was found to play a pivotal role in maintaining periodontal homeostasis. The aim of this study was to explore the function of PLAP-1 in the periodontally hypofunctional tissue turnover. Methods:After extracting left maxillary molars in mice, the left and right mandibular molars were distributed into hypofunction group (HG) and control group (CG), respectively. Mice were sacrificed for radiographic, histological, and molecular biological analyses after 1, 4 and 12 weeks. In vitro, dynamic compression was applied using Flexcell FX-5000 Compression System to simulate intermittent occlusal force. The expression of PLAP1 in loaded and unloaded human periodontal ligament cells (hP-DLCs) was compared, and its molecular biological effects were further explored by small interfering RNA (siRNA) targeting PLAP1. Results:In vivo, fiber disorder in periodontal ligament (PDL), bone apposition at furcation regions, and bone resorption in alveolar bone were illustrated in the HG compared with the CG. In addition, PLAP-1 positive area decreased significantly in PDL following occlusal unloading. In vitro, the loss of compressive loading relatively downregulated PLAP1 expression, which was essential to promote collagen I but inhibit osterix and osteocalcin expression in hPDLCs.Conclusions: PLAP-1 presumably plays a pivotal role in occlusal force-regulated periodontal homeostasis by facilitating collagen fiber synthesis in hPDLCs and suppressing excessive osteoblast differentiation, further preventing teeth from overeruption.

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Section: Discussionsupporting

confidence: 65%

Periodontal ligament‐associated protein‐1 engages in teeth overeruption and periodontal fiber disorder following occlusal hypofunction

Chen

Luo

Xie

et al. 2022

J of Periodontal Research

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“…23,24 Deposition of cellular cementum can increase root volume and delay root resorption. 24,25 However, Li et al 15 found that the final mesial root volume in the immature experimental group was significantly less than that in the control group. Considering the differences in the experimental design, such as the control setting, observation time points, and the volume definition during measurement, the comparability between the two studies is low.…”

Section: Discussionmentioning

confidence: 99%

Micro-computed tomography evaluation of the effects of orthodontic force on immature maxillary first molars and alveolar bone mineral density of Sprague–Dawley rats

Wang

Zhang

et al. 2023

Korean J Orthod

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Objective: To investigate changes in the immature teeth of Sprague-Dawley rats during orthodontic treatment and to explore the changes in the peri-radicular alveolar bone through micro-computed tomography (CT). Methods: Twentyfive 26-day-old male Sprague-Dawley rats were included. The maxillary left first molar was moved mesially under a continuous force of 30 cN, and the right first molar served as the control. After orthodontic treatment for 7, 14, 21, 28, and 42 days, the root length, tooth volume, and alveolar bone mineral density (BMD) around the mesial root were measured through micro-CT. Results: The immature teeth continued to elongate after application of orthodontic force. The root length on the force side was significantly smaller than that on the control side, whereas the differences in the volume change between both sides were not statistically significant. Alveolar bone in the coronal part of the compression and tension sides showed no difference in BMD between the experimental and control groups. The BMD of the experimental group decreased from day 14 to day 42 in the apical part of the compression side and increased from day 7 to day 42 in the apical part of the tension side. The BMD of the experimental group decreased in the root apex part on day 7. Conclusions: The root length and volume of immature teeth showed continued development under orthodontic forces. Alveolar bone resorption was observed on the compression side, and bone formation was observed on the tension side.

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“…Another advantage of the RMA procedure is its reversibility. Tooth extraction, bite-raising appliances and grinding of the molars have been introduced to achieve malocclusion or hypofunction models in rodents [31][32][33][34][35]. However, these procedures involve irreversible pathological changes to masticatory activity.…”

Section: Discussionmentioning

confidence: 99%

Newly developed mastication activity reduction procedure rapidly induces abnormal atrophic change of the mandibular condyle in young and elder experimental animal models

et al. 2020

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This study was performed to develop a new rat model of reduced masticatory activity in order to assess the effect of this reduction on the morphology of the temporomandibular joint (TMJ) over time. Female rats were used, and ovariectomy was performed to simulate aged/ postmenopausal status. Twenty-four SD rats aged 6 weeks were divided into four groups: ovariectomy/sham procedure (Ov/S); ovariectomy/ reduced masticatory activity (Ov/RMA); non-Ov/S (NO/S); and non-Ov/ RMA (NO/RMA). The RMA procedure involved grinding down the edges of the upper and mandibular incisors by about 3 mm and supplying the rats with a powdered diet. The bilateral TMJ was examined by microcomputed tomography at 0, 1, 2, 4, 6, and 8 weeks after the start of RMA. Condylar width was greater in the NO/S group than in the Ov/S group after the 2nd week, showing that ovariectomy reduced the width of the condyle. After the 2nd week, significant differences in condylar width were apparent between the NO/S and NO/RMA groups, and between the Ov/S and Ov/RMA groups. This RMA procedure appeared to provide a good model of reduced masticatory activity. The present findings in female rats suggest that reduction of appropriate mastication activity in the growth period results in poor growth of the mandibular condyle and immediately induces atrophy of the mandibular condyle under conditions simulating aged/postmenopausal status.

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Impact of hypofunctional occlusion on upper and lower molars after cessation of root development in adult mice

Cited by 3 publications

References 27 publications

Periodontal ligament‐associated protein‐1 engages in teeth overeruption and periodontal fiber disorder following occlusal hypofunction

Periodontal ligament‐associated protein‐1 engages in teeth overeruption and periodontal fiber disorder following occlusal hypofunction

Micro-computed tomography evaluation of the effects of orthodontic force on immature maxillary first molars and alveolar bone mineral density of Sprague–Dawley rats

Newly developed mastication activity reduction procedure rapidly induces abnormal atrophic change of the mandibular condyle in young and elder experimental animal models

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