Impact of history of periodontitis on gene expression of bone-related factors in young patients

Cirano, Fabiano Ribeiro; Pimentel, Suzana Peres; Ribeiro, Fernanda Vieira; Casati, Márcio Zaffalon; Casarin, Renato Corrêa Viana; Gallafassi, Daniel Freire; Nishii, Denise; Corrêa, Mônica Grazieli

doi:10.1590/1807-3107bor-2020.vol34.0014

Cited by 5 publications

(3 citation statements)

References 40 publications

(52 reference statements)

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“…108 Collectively, TGFB1, BMP2, and BMP7 have an osteogenic effect via differentiation of MSCs to the Ob phenotype. 108,109 In existing rodent models, MSC recruitment and angiogenic markers are often expressed at their highest at the 7 and 14 day time points, while in this model, these angiogenic and MSC recruitment markers (Tgf b1, Bmp2, and Bmp7) are expressed at their highest at the 2 day time point. 76,80,88 This is again supporting evidence that bone remodeling may be occurring at a faster rate in this model than in other rodent models.…”

Section: Discussionmentioning

confidence: 91%

Cellular and Molecular Dynamics during Early Oral Osseointegration: A Comprehensive Characterization in the Lewis Rat

Wheelis

Biguetti

Natarajan

et al. 2021

ACS Biomater. Sci. Eng.

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Objective: There is a need to improve the predictability of osseointegration in implant dentistry. Current literature uses a variety of in vivo titanium (Ti) implantation models to investigate failure modes and test new materials and surfaces. However, these models produce a variety of results, making comparison across studies difficult. The purpose of this study is to validate an oral osseointegration in the Lewis rat to provide a reproducible baseline to track the inflammatory response and healing of Ti implants. Methods: Ti screws (0.76 mm Ø × 2 mm length) were implanted into the maxillary diastema of 52 adult male Lewis rats. Peri-implant tissues were evaluated 2, 7, 14, and 30 days after implantation (n = 13). Seven of the 13 samples underwent microtomographic analysis, histology, histomorphometry, and immunohistochemistry to track healing parameters. The remaining six samples underwent quantitative polymerase chain reaction (qPCR) to evaluate gene expression of inflammation and bone remodeling markers over time. Results: This model achieved a 78.5% success rate. Successful implants had a bone to implant contact (BIC)% of 68.86 ± 3.15 at 30 days on average. Histologically, healing was similar to other rodent models: hematoma and acute inflammation at 2 days, initial bone formation at 7, advanced bone formation and remodeling at 14, and bone maturation at 30. qPCR indicated the highest expression of bone remodeling and inflammatory markers 2−7 days, before slowly declining to nonsurgery control levels at 14−30 days. Conclusion: This model combines cost-effectiveness and simplicity of a rodent model, while maximizing BIC, making it an excellent candidate for evaluation of new surfaces.

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Section: Discussionmentioning

confidence: 91%

Cellular and Molecular Dynamics during Early Oral Osseointegration: A Comprehensive Characterization in the Lewis Rat

Wheelis

Biguetti

Natarajan

et al. 2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…However in the present study, by comparing transforming growth factor beta level from baseline to 4weeks this result is parallel to the previous study observed that TGF-β levels increased significantly in the treated group reported that an increase in TGF-β levels was associated with a decrease in periodontal inflammation, leading to improved clinical outcomes (38) . With other study findings suggesting that high TGF-β production may be a protective factor for periodontitis, accelerating connective tissue remodeling and angiogenesis, which could contribute to inflammatory regulation and/or to bone remodeling events (39) .…”

Section: Discussionmentioning

confidence: 60%

Effect of Lepidium Sativum Gel as an Adjunct to Non- Surgical Treatment in Management of Periodontitis Patients Stage (II, III) and Grade (A, B)

et al. 2021

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Purpose: The current study was conducted to elaborate the clinical and transforming growth factor beta biomarker effect of local application of lepidium sativum gel as an adjunct to scaling and root planing in the therapeutic management of periodontitis patients stage( II, III) and grade(A, B). Subjects and Methods: The current study was conducted on twenty patients were selected in this study with stage (II, III) and grade (A, B) periodontitis. Patients were randomly divided into two group's ten subjects on each. Group A: (control group) were treated with scaling and root planing alone, group B: (study group) were treated with scaling and root planing with local delivery of lepidium sativum gel. Results: study group showed a significant increase in the clinical parameter like clinical attachment level (CAL), probing depth (PD), bleeding on probing (BOP) and plaque index (PI). Clinical parameters were recorded after one month and three months, transforming growth factor beta biomarker level after one week and four weeks in comparison with control group follow up. Conclusion: Adjunctive use of lepidium sativum with non-surgical therapy showed an improvement in the treatment outcome of periodontitis patient's stage (II, III) and grade (A, B).

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“…Gene therapy has been developed to expose multiple factors to damaged surfaces for long periods of time and maintain constant protein levels, promoting recovery (202). Many studies have found that gene expression of cells in periodontitis tissues changes (203,204). And genotypes are also important for susceptibility to periodontitis (205).…”

Section: Gene Therapymentioning

confidence: 99%

Immunomodulation in the Treatment of Periodontitis: Progress and Perspectives

Yang

Pang

et al. 2021

Front. Immunol.

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Periodontitis is one of the most common dental diseases. Compared with healthy periodontal tissues, the immune microenvironment plays the key role in periodontitis by allowing the invasion of pathogens. It is possible that modulating the immune microenvironment can supplement traditional treatments and may even promote periodontal regeneration by using stem cells, bacteria, etc. New anti-inflammatory therapies can enhance the generation of a viable local immune microenvironment and promote cell homing and tissue formation, thereby achieving higher levels of immune regulation and tissue repair. We screened recent studies to summarize the advances of the immunomodulatory treatments for periodontitis in the aspects of drug therapy, microbial therapy, stem cell therapy, gene therapy and other therapies. In addition, we included the changes of immune cells and cytokines in the immune microenvironment of periodontitis in the section of drug therapy so as to make it clearer how the treatments took effects accordingly. In the future, more research needs to be done to improve immunotherapy methods and understand the risks and long-term efficacy of these methods in periodontitis.

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Impact of history of periodontitis on gene expression of bone-related factors in young patients

Cited by 5 publications

References 40 publications

Cellular and Molecular Dynamics during Early Oral Osseointegration: A Comprehensive Characterization in the Lewis Rat

Cellular and Molecular Dynamics during Early Oral Osseointegration: A Comprehensive Characterization in the Lewis Rat

Effect of Lepidium Sativum Gel as an Adjunct to Non- Surgical Treatment in Management of Periodontitis Patients Stage (II, III) and Grade (A, B)

Immunomodulation in the Treatment of Periodontitis: Progress and Perspectives

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