Impact of gut microbiota on diabetes mellitus

Blandino, G; Inturri, Rosanna; Lazzara, Francesca; Rosa, Michelino Di; Malaguarnera, Lucia

doi:10.1016/j.diabet.2016.04.004

Cited by 197 publications

(134 citation statements)

References 122 publications

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“…We obtained data on structural changes of the intestinal epithelial barrier of the duodenum of rats in burn skin injury under experimental streptozotocin-induced diabetes mellitus, indicating an increase in the paracellular permeability of the barrier. In animals of the second experimental group, intestinal microbes were found to penetrate the defective intestinal epithelial barrier of the duodenum, which is consistent with the data [3] on the role of intestinal microbiota translocation in the pathogenesis of diabetes.…”

Section: Discussionsupporting

confidence: 89%

“…With the development of burn disease, the translocation of pathogenic microflora from the lumen of the intestine to other organs leads to deepening of the systemic inflammatory response syndrome, hypermetabolism, multiple organ failure, sepsis. Such translocation is a factor in the worsening of diabetes mellitus [3,19], which was once witnessed by Vaarala O. et al [28], calling the intricate relationship between gut microbiota, permeability of the intestinal epithelial barrier and mucosal immunity a "perfect storm" for diabetes.…”

Section: Discussionmentioning

confidence: 99%

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Structural changes of the intestinal epithelial barrier of the duodenum of rats in burn injury of skin under experimental streptozotocin-induced diabetes mellitus

Tymoshenko

2019

Rep. of Morph.

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The aim of the study was to study the structural changes of the intestinal epithelial barrier in the duodenum in burn injury of skin in rat under experimental streptozotocin-induced diabetes mellitus. The study was carried out on laboratory white adult rats-males weighing 180-210 g. The control group consisted of 21 animals without somatic pathology, the first experimental group was 21 rats with burn skin injury, the second experimental group was 21 rats with burn skin and experimental streptozotocin-induced diabetes mellitus. The experimental diabetes model was reproduced by administering streptozotocin to the rats intraperitoneally at a single dose of 50 mg/kg. Thermal burn skin damage in rats corresponded to II – A-B degrees of dermal surface burn (according to the old classification III – A degree) with a total area of 21-23 % of the body surface with the development of burn shock. Duodenum was selected for morphological studies, fragments of which were processed by conventional methods of light and electron microscopy. The main criteria for assessing damage to the duodenal mucosa enterocytes were the results of a study comparing histological and ultrastructural data in dynamics at 7, 14, and 21 days after skin burns. The results of the studies showed that the base of damage to the intestinal epithelial barrier of the duodenum are deep destructive changes, which after 21 days (in the stage of septicotoxemia), as a rule, are not reverse and develop on the background of significant intoxication of the body. There was a decrease in the number of tight junctions in the intestinal epithelial barrier of the duodenum of rats of the first and second experimental groups and a loss of ordering (acquisition of some chaotic nature) of their localization as the time after burn injury increased. For the most part, the intestinal epithelial barrier loses the integrity of the cellular component with partial preservation of the basement membrane (the first occurs both due to necrosis of the enterocytes with a brush border and due to complete destruction of goblet cells). In all cases, defects exist in the intestinal epithelial barrier, which are potential pathways for paracellular translocation of the pathogenic contents of the duodenum. It is not inconceivable that part of this intestinal pathogenic content can be translocated also by partially damaged cells. Evidence of the latter is the presence of microbial bodies in the cytoplasm of cells with partially destroyed plasmalemma (but preserved organelles and nucleus). An adaptive mechanism for ensuring the repair of damaged enterocytes is selective autophagy, which acts as a factor in the recycling of destroyed organelles and the cytoplasmic matrix, aimed primarily at maintaining cell viability.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Structural changes of the intestinal epithelial barrier of the duodenum of rats in burn injury of skin under experimental streptozotocin-induced diabetes mellitus

Tymoshenko

2019

Rep. of Morph.

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“…These patients showed higher levels of Lactobacillus spp. than non-diabetic patients [18]. It was detected that the Lactobacillus levels were significantly rising in patients with diabetes than in the healthy group.…”

Section: Discussionmentioning

confidence: 78%

Exploitation of Absolute qPCR to Estimate Lactobacillus and Bifidobacterium Count in Human Gut as Indicator of Diabetic Mellitus Complication

Al-Rawe

Suleiman

2020

eijs

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Diabetes mellitus is a form of metabolic disorder where patients are incapable to organize glucose metabolism. The most common types are Type I and Type II, constituting about 10% and 90% of cases, respectively. The cause of type I diabetes, which usually spreads in children and adolescents, is the disability of the endocrine system to produce insulin. On the other hand, The most common type of diabetes, type II diabetes, is often presented in adults. It is usually presented as a collection of insulin deficiency and insulin resistance. This work was done to estimate the count of microbiota in diabetics to find an appraoch for detection and follow-up treatment. The count of two types of bacteria Lactobacillus and Bifidobacterium was determined using qPCR based on the standard curve that was created from the serial decimal dilution of samples containing an unknown number of bacteria taken from probiotic capsules. The main results of this study show that the Lactobacillus count was affected by diabetes types, where a decrease was observed in the mean value in the case of diabetes type I group (32978.13) compared with the control group (610680.26). The mean value in diabetes type II was close to that of the control group (682199.27). While, the count of the Bifidobacterium showed a significant reduction in the mean value in both type I and type II diabetes groups (7521.70, 51880.82, respectively), compared with the control group (63405999.00).

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“…Diabetes is mainly characterized by hyperglycemia, in particular, type 2 diabetes is the most common form of diabetes accounting for approximately 90% of all cases . Accumulating evidence indicates that the intestinal microflora composition is associated with the development of type 2 diabetes, which has been considered to be a new therapeutic target for prevention and management of the disease . Hence, effective control of blood glucose levels and regulation of intestinal microbial community are important aspects of preventing or reversing the onset of type 2 diabetes.…”

Section: Introductionmentioning

confidence: 99%

Regulatory Efficacy of Brown Seaweed Lessonia nigrescens Extract on the Gene Expression Profile and Intestinal Microflora in Type 2 Diabetic Mice

Zhao

Yang

Chen

et al. 2018

Molecular Nutrition Food Res

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Impact of gut microbiota on diabetes mellitus

Cited by 197 publications

References 122 publications

Structural changes of the intestinal epithelial barrier of the duodenum of rats in burn injury of skin under experimental streptozotocin-induced diabetes mellitus

Structural changes of the intestinal epithelial barrier of the duodenum of rats in burn injury of skin under experimental streptozotocin-induced diabetes mellitus

Exploitation of Absolute qPCR to Estimate Lactobacillus and Bifidobacterium Count in Human Gut as Indicator of Diabetic Mellitus Complication

Regulatory Efficacy of Brown Seaweed Lessonia nigrescens Extract on the Gene Expression Profile and Intestinal Microflora in Type 2 Diabetic Mice

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