Impact of Green Tea Extract on Intraradicular Dentin Wettability and Bond Strength of Resin-Based Sealer Following Two Chelating Agents

Abstract

Purpose: To evaluate the effect of 3% green tea extract (GTE) on wettability, depth of penetration, and push out bond strength (PBS) of AH Plus sealer to intraradicular dentin following the use of 0.2% Nano chitosan or 17% EDTA. Materials and methods: Twenty human radicular dentin segments were used for wettability assessment. Following immersion in 2.6% NaOCl solution, samples were divided into 2 groups (n=10) according to the chelating agent used: group I: 0.2% Nano chitosan, and group II: 17% EDTA, each used for 3 min, then the contact angle (CA) was measured. The samples were further immersed in 3% GTE solution for 5 min followed by CA measurement. Twenty human mandibular premolars were prepared up to # X4 ProTaper Next files, then divided into two groups as mentioned previously. Each group was subdivided into two subgroups based on the final flush used (n=5); subgroups A and B; no treatment, and 3% GTE, respectively. Samples were obturated by cold lateral compaction using AH Plus sealer and gutta-percha. PBS was measured using a universal testing machine and sealer penetration was assessed using confocal laser scanning microscopy. Results: Using 3% GTE following the use of 0.2% Nano chitosan significantly increased the wettability and PBS of AH Plus sealer to intraradicular dentin, while its use following 17% EDTA significantly decreased the wettability and PBS of AH Plus sealer. Using GTE increased the maximum depth of AH Plus sealer penetration into the dentinal tubules. Conclusion: GTE improved the wettability, depth of penetration, and push out bond strength of AH Plus sealer to intraradicular dentin treated with Nano-chitosan. Nevertheless, it adversely affected those properties of AH Plus sealer to EDTA-treated dentin.