Impact of gas–liquid mass transfer on organic acids production by Corynebacterium glutamicum in unbaffled shake flasks

Kaboré, Abdoul Karim; Olmos, Éric; Blanchard, Fabrice; Fick, Michel; Delaunay, Stéphane; Guédon, Emmanuel

doi:10.1016/j.bej.2015.06.003

Cited by 4 publications

(11 citation statements)

References 53 publications

(72 reference statements)

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“…With this last strain, an organic acid production related to the oxygenation level was also observed. The maximal succinate and acetate production corresponded to k L a values between 10 and 19 h −1 , and between 19 and 33 h −1 , respectively, and a similar relationship between the k L a and the maximal biomass concentration was measured . Only a reduction in the conversion yields of glucose into organic acids was determined with C. glutamicum 2262.…”

Section: Discussionsupporting

confidence: 61%

“…To investigate the impact of oxygenation level on the physiological response of the wild‐type strain, C. glutamicum 2262, batch cultures were performed in unbaffled shake flasks. As it was previously validated for a mutant strain of C. glutamicum 2262 , the correlation of Seletzky et al. was used for the evaluation of applied k L a during the cultures.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, using a ldhA ‐deleted strain of C. glutamicum 2262, Kaboré et al. demonstrated the very good agreement between experimental and estimated values with a relative difference less than 10%.

k_{L} a = 0.024 \times N^{1.16} \times V^{- 0.83} \times d_{0}^{0.38} \times d^{1.92}

This correlation was validated for a broad range of the operating parameters ( N : 50–500 rpm; V : 4–20%; d 0 : 1.25‐10 cm, and flask volumes between 50 and 1000 mL), and the accuracy was 30% .…”

Section: Methodsmentioning

confidence: 96%

“…Concerning the production of succinate by C. glumaticum , a decrease in the succinate concentration, in micro‐aerobic environments, was previously noticed at the end of cultures using a strain deleted for LDH. This re‐consumption was related, according to the authors, to the glucose depletion in the culture medium . Moreover, recently, Conrady et al.…”

Section: Introductionmentioning

confidence: 87%

“…The dry cell concentration was calculated using the following relation:

false(g DW L^{- 1} false) = 0.35 \times O {normalD}_{570 nm}

The lactate, succinate, acetate, pyruvate, and fumarate concentrations were measured by HPLC according to the method described by Kaboré et al. .…”

Section: Methodsmentioning

confidence: 99%

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Corynebacterium glutamicum, a natural overproducer of succinic acid?

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Kaboré

Olmos

et al. 2020

Engineering in Life Sciences

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Corynebacterium glutamicum is well known as an important industrial amino acid producer. For a few years, its ability to produce organic acids, under micro-aerobic or anaerobic conditions was demonstrated. This study is focused on the identification of the culture parameters influencing the organic acids production and, in particular, the succinate production, by this bacterium. Corynebacterium glutamicum 2262, used throughout this study, was a wild-type strain, which was not genetically designed for the production of succinate. The oxygenation level and the residual glucose concentration appeared as two critical parameters for the organic acids production. The maximal succinate concentration (4.9 g L −1 ) corresponded to the lower k L a value of 5 h −1 .Above 5 h −1 , a transient accumulation of the succinate was observed. Interestingly, the stop in the succinate production was concomitant with a lower threshold glucose concentration of 9 g L −1 . Taking into account this threshold, a fed-batch culture was performed to optimize the succinate production with C. glutamicum 2262. The results showed that this wild-type strain was able to produce 93.6 g L −1 of succinate, which is one of the highest concentration reported in the literature. K E Y W O R D SCorynebacterium glutamicum, oxygen transfer, residual glucose concentration, succinate Abbreviations: DOC, dissolved oxygen concentration; k L a, volumetric mass transfer coefficient; LDH, lactate dehydrogenase.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionsupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%