Corynebacterium glutamicum, a natural overproducer of succinic acid?

Abstract

Corynebacterium glutamicum is well known as an important industrial amino acid producer. For a few years, its ability to produce organic acids, under micro-aerobic or anaerobic conditions was demonstrated. This study is focused on the identification of the culture parameters influencing the organic acids production and, in particular, the succinate production, by this bacterium. Corynebacterium glutamicum 2262, used throughout this study, was a wild-type strain, which was not genetically designed for the production of succinate. The oxygenation level and the residual glucose concentration appeared as two critical parameters for the organic acids production. The maximal succinate concentration (4.9 g L −1 ) corresponded to the lower k L a value of 5 h −1 .Above 5 h −1 , a transient accumulation of the succinate was observed. Interestingly, the stop in the succinate production was concomitant with a lower threshold glucose concentration of 9 g L −1 . Taking into account this threshold, a fed-batch culture was performed to optimize the succinate production with C. glutamicum 2262. The results showed that this wild-type strain was able to produce 93.6 g L −1 of succinate, which is one of the highest concentration reported in the literature. K E Y W O R D SCorynebacterium glutamicum, oxygen transfer, residual glucose concentration, succinate Abbreviations: DOC, dissolved oxygen concentration; k L a, volumetric mass transfer coefficient; LDH, lactate dehydrogenase.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

The Industrial Organism Corynebacterium glutamicum Requires Mycothiol as Antioxidant to Resist Against Oxidative Stress in Bioreactor Cultivations

“…The Gram-positive soil bacterium Corynebacterium glutamicum is widely used as anindustrial workhorse primarily for the production of L-glutamate and L-lysine [ 1 ], and has been genetically engineered as a broad platform for production of several important industrial products [ 2 , 3 ]. Currently, C. glutamiucm is mostly used for aerobic production processes, but its facultative anaerobic metabolism allows to design efficient two-stage processes for the production of reduced chemicals including an aerobic growth phase and an anaerobic production phase [ 4 , 5 ].…”

Synthetic Biology Toolkits and Metabolic Engineering Applied in Corynebacterium glutamicum for Biomanufacturing

“…Recently, C. glutamicum has been widely used to produce amino acids, organic acids, triacylglycerols, vitamins, alcohols, carotenoids, and various high value-added products. Table summarized the strategies and main products of the construction of C. glutamicum strains into a cellular microbial factory.…”

“…Microbial production has many advantages, including low cost, low energy consumption, easy removal of byproducts, and other superiorities . As a “Generally Regarded as Safe” bacterium, Corynebacterium glutamicum has great potential in producing amino acids, organic acids, and other high value-added products. Moreover, compared with other microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Bacillus subtilis, C. glutamicum has its distinct physiological properties.…”

From Microbial Succinic Acid Production to Polybutylene Bio‐Succinate Synthesis

“…Among the various microbes tested for bio-SA production [8,9], the Gram-positive soil bacterium Corynebacterium glutamicum shows exceptional performance under aerobic and anaerobic process conditions, both as wild-type and metabolically engineered variants [10][11][12][13]. With one of the currently best performing strains, C. glutamicum BOL-3/pAN6-gap, bio-SA titer of 144 g L -1 and yield of 1.67 mol SA mol GLC -1 could be achieved [10].…”