Corynebacterium glutamicum is an important workhorse in industrial white biotechnology. It has been widely applied in the producing processes of amino acids, fuels, and diverse value-added chemicals. With the continuous disclosure of genetic regulation mechanisms, various strategies and technologies of synthetic biology were used to design and construct C. glutamicum cells for biomanufacturing and bioremediation. This study mainly aimed to summarize the design and construction strategies of C. glutamicum-engineered strains, which were based on genomic modification, synthetic biological device-assisted metabolic flux optimization, and directed evolution-based engineering. Then, taking two important bioproducts (N-acetylglucosamine and hyaluronic acid) as examples, the applications of C. glutamicum cell factories were introduced. Finally, we discussed the current challenges and future development trends of C. glutamicumengineered strain construction.
l-Histidine is an essential
proteinogenic amino acid in
food with extensive applications in the pharmaceutical field. Herein,
we constructed a Corynebacterium glutamicum recombinant strain for efficient biosynthesis of l-histidine.
First, to alleviate the l-histidine feedback inhibition,
the ATP phosphoribosyltransferase mutant HisGT235P‑Y56M was constructed based on molecular docking and high-throughput screening,
resulting in the accumulation of 0.83 g/L of l-histidine.
Next, we overexpressed rate-limiting enzymes including HisGT235P‑Y56M and PRPP synthetase and knocked out the pgi gene
in the competing pathway, which increased the l-histidine
production to 1.21 g/L. Furthermore, the energy status was optimized
by decreasing the reactive oxygen species level and enhancing the
supply of adenosine triphosphate, reaching a titer of 3.10 g/L in
a shake flask. The final recombinant strain produced 5.07 g/L of l-histidine in a 3 L bioreactor, without the addition of antibiotics
and chemical inducers. Overall, this study developed an efficient
cell factory for l-histidine biosynthesis by combinatorial
protein engineering and metabolic engineering.
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