Impact of exogenous caffeine on morphological, biochemical, and ultrastructural characteristics of Nicotiana tabacum

Alkhatib, Rami; Alkhatib, Batool; AL-Quraan, Nisreen A.; AL-Eitan, Laith N.; Abdo, Nour; Muhaidat, Riyadh

doi:10.1007/s10535-016-0600-z

Cited by 13 publications

(9 citation statements)

References 45 publications

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“…Furthermore, the synthesis of osmolytes in plants undergoing abiotic stress is increased significantly to adapt with the stress. Previously, we demonstrated that a drastic reduction of P N is accompanied by an increase in sugar content in the leaves of 1000 and 5000 μM caffeine-treated plants [18]. Interestingly, our data showed that no significant change in sugar accumulation occurred in plants treated with (Fe 3 O 4 ) NPs compared to control plants.…”

Section: Discussioncontrasting

confidence: 42%

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Alkhatib

Abdo

et al. 2019

BMC Plant Biol

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Background Because of their broad applications in our life, nanoparticles are expected to be present in the environment raising many concerns about their possible adverse effects on the ecosystem of plants. The aim of this study was to examine the effect of different sizes and concentrations of iron oxide nanoparticles [(Fe 3 O 4 ) NPs] on morphological, physiological, biochemical, and ultrastructural parameters in tobacco ( Nicotiana tabacum var.2 Turkish). Results Lengths of shoots and roots of 5 nm-treated plants were significantly decreased in all nanoparticle-treated plants compared to control plants or plants treated with any concentration of 10 or 20 nm nanoparticles. The photosynthetic rate and leaf area were drastically reduced in 5 nm (Fe 3 O 4 ) NP-treated plants of all concentrations compared to control plants and plants treated with 10 or 20 nm (Fe 3 O 4 ) NPs. Accumulation of sugars in leaves showed no significant differences between the control plants and plants treated with iron oxide of all sizes and concentrations. In contrast, protein accumulation in plants treated with 5 nm iron oxide dramatically increased compared to control plants. Moreover, light and transmission electron micrographs of roots and leaves revealed that roots and chloroplasts of 5 nm (Fe 3 O 4 ) NPs-treated plants of all concentrations were drastically affected. Conclusions The size and concentration of nanoparticles are key factors affecting plant growth and development. The results of this study demonstrated that the toxicity of (Fe 3 O 4 ) NPs was clearly influenced by size and concentration. Further investigations are needed to elucidate more about NP toxicity in plants, especially at the molecular level. Electronic supplementary material The online version of this article (10.1186/s12870-019-1864-1) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 42%

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Alkhatib

Abdo

et al. 2019

BMC Plant Biol

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“…However, the physiological and anatomical effects of caffeine on plant roots and leaves have not been investigated until recently. Our 2016 study reported that caffeine stress caused changes in morphological, biochemical, and ultrastructural characteristics of Nicotiana tabacum leaves (Alkhatib et al 2016). In this current study, we hypothesise that plants grown in high concentrations of caffeine exhibit physiological, structural, and ultrastructural alterations both in leaf and root cells.…”

Section: Introductionmentioning

confidence: 54%

“…The temperature was 30°C (day) and 23°C (night), and the relative humidity (RH) was approximately 60%. The seedlings were grown in the light and in the dark under circadian illumination of 16-h light/8-h dark (Alkhatib et al 2016). A pilot study was conducted with different concentrations of caffeine (AZ Chem for chemicals, cat#1590250) (25, 50, 100, 200, 500, 1,000; 5,000; and 20,000 µM).…”

Section: Methodsmentioning

confidence: 99%

“…Mature root and leaf samples (the third and fourth from the top of each plant) (0.5-1.0 cm) from caffeine-treated and control plants were prepared for light and transmission microscopy following the same protocol as described by Alkhatib et al (2016). Briefly, samples were fixed in 2.5% glutaraldehyde buffered at pH 7.4 in 0.1 M cacodylate buffer overnight at 4°C.…”

Section: Light and Transmission Electron Microscopymentioning

confidence: 99%

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Physio-anatomical responses of tobacco under caffeine stress

Alkhatib¹,

Alkhatib²,

AL-Eitan³

et al. 2018

Photosynt.

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Caffeine, a purine alkaloid, is reported to act both as an inducer or inhibitor to plant growth in various species. The aim of this study was to examine the effect of exogenous caffeine on tobacco (Nicotiana tabacum) plants, a plant that does not naturally synthesise caffeine. A hydroponic experiment was carried out in a growth chamber for 14 d using Hoagland's solution supplemented with 0 (control), 25, 50, 100, 1,000; and 5,000 µM caffeine. None of the investigated caffeine concentrations significantly decreased the net photosynthetic rate except the highest concentrations of 1,000 and 5,000 µM. Light microscopy of thick-sectioned roots showed that 1,000 µM and 5,000 µM caffeine-treated plants possessed deformed epidermal cells, reduced number of cortical cells, and deformed vascular tissues with cells exhibiting thickened xylem walls as compared with control plants. Moreover, transmission electron micrographs of roots revealed that mitochondria and the plasma membrane were affected.

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“…). Several studies have reported that this phenomenon in most plant species under different abiotic stress (Gilbert et al ; Balibrea et al ; Pattanagul & Thitisaksakul ; Alkhatib et al ). Dhanapackiam & Ilyas () reported that accumulation of organic solutes could play a vital role in increasing internal osmotic pressure, allowing the plant to cope with some abiotic stress.…”

Section: Discussionmentioning

confidence: 89%

Effect of lead on the physiological, biochemical and ultrastructural properties of Leucaena leucocephala

et al. 2019

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Heavy metals are characterised by a relatively high density and cause genotoxic, cytotoxic and mutagenic effects on plants, animals and humans. Lead (Pb) is one of the heavy metals that causes toxicity to plants and animals.• This experiment was conducted using a hydroponic technique to study the effects of Pb(NO 3 ) 2 on physiological, biochemical and ultrastructural characteristics in Leucaena leucocephala seedlings. Plants were grown in a growth chamber for 21 days in Hoagland's solution supplemented with 0 (control), 25, 50, 100, 300, 500 and 700 µM Pb(NO 3 ) 2 .• Shoot heights as well as root lengths decreased significantly in Pb-treated plants with 300, 500 and 700 µM. In Pb-treated plants with high Pb concentrations, photosynthesis rate (P N ), stomatal conductance (g s ) and transpiration rate (E) decreased. Total protein and carbohydrate content in Pb-treated plants with 300, 500 and 700 µM increased significantly in leaves. Moreover, in Pb-treated plants with 300, 500 and 700 µM Pb(NO 3 ) 2 , mesophyll cells had enlarged chloroplasts with disrupted thylakoid membranes associated with large starch grains. In contrast, Pb treatments with 25, 50 µM and 100 µM were not toxic to the plants. Thick sections of roots of Pb-treated plants with 300, 500 and 700 µM Pb showed distinct changes in structure of epidermal and cortical cells. Moreover, thin sections of roots of Pb-treated plants with 300, 500 and 700 µM Pb had thickened walls of xylem cells.• These results will shed more light in understanding the effects of heavy metal stress on plants.

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Impact of exogenous caffeine on morphological, biochemical, and ultrastructural characteristics of Nicotiana tabacum

Cited by 13 publications

References 45 publications

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Physio-anatomical responses of tobacco under caffeine stress

Effect of lead on the physiological, biochemical and ultrastructural properties of Leucaena leucocephala

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