Physio-anatomical responses of tobacco under caffeine stress

Alkhatib, Rami; Alkhatib, Batool; AL-Eitan, Laith N.; Abdo, Nour; Tadros, Maher J.; Bsoul, Emad

doi:10.1007/s11099-018-0798-4

Cited by 10 publications

(8 citation statements)

References 29 publications

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“…Mature root and leaf samples (0.5–1.0 cm) from control and NP -treated plants were prepared for light and transmission microscopy following the same protocol as described by Alkhatib et al [30]. Briefly, samples were fixed in 2.5% (m/v) glutaraldehyde and post-fixed in osmium tetroxide (EMS, Hatfield, PA, USA).…”

Section: Methodsmentioning

confidence: 99%

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Alkhatib

Abdo

et al. 2019

BMC Plant Biol

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Background Because of their broad applications in our life, nanoparticles are expected to be present in the environment raising many concerns about their possible adverse effects on the ecosystem of plants. The aim of this study was to examine the effect of different sizes and concentrations of iron oxide nanoparticles [(Fe 3 O 4 ) NPs] on morphological, physiological, biochemical, and ultrastructural parameters in tobacco ( Nicotiana tabacum var.2 Turkish). Results Lengths of shoots and roots of 5 nm-treated plants were significantly decreased in all nanoparticle-treated plants compared to control plants or plants treated with any concentration of 10 or 20 nm nanoparticles. The photosynthetic rate and leaf area were drastically reduced in 5 nm (Fe 3 O 4 ) NP-treated plants of all concentrations compared to control plants and plants treated with 10 or 20 nm (Fe 3 O 4 ) NPs. Accumulation of sugars in leaves showed no significant differences between the control plants and plants treated with iron oxide of all sizes and concentrations. In contrast, protein accumulation in plants treated with 5 nm iron oxide dramatically increased compared to control plants. Moreover, light and transmission electron micrographs of roots and leaves revealed that roots and chloroplasts of 5 nm (Fe 3 O 4 ) NPs-treated plants of all concentrations were drastically affected. Conclusions The size and concentration of nanoparticles are key factors affecting plant growth and development. The results of this study demonstrated that the toxicity of (Fe 3 O 4 ) NPs was clearly influenced by size and concentration. Further investigations are needed to elucidate more about NP toxicity in plants, especially at the molecular level. Electronic supplementary material The online version of this article (10.1186/s12870-019-1864-1) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Alkhatib

Abdo

et al. 2019

BMC Plant Biol

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“…The seedlings were removed from the soil and their roots carefully washed with distilled water. Uniform plants of equal height and number of leaves were placed in Hoagland's solution (Caisson Laboratories, UT, USA) and grown hydroponically in a growth chamber (Bionex, model VS‐3DM, Korea) as described by Alkhatib et al (). Different concentrations of Pb were added in the form of Pb(NO 3 ) 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Leaves and roots from control and Pb‐treated plants were cut into small pieces (0.5–1.0 cm) using a single‐edge blade (EMS, Hatfield, PA, USA). Control and Pb‐treated plants were prepared for light and transmission electron microscopy (TEM) following the protocol described by Alkhatib et al (). Briefly, samples were fixed overnight at 4 °C in 2.5% glutaraldehyde in 0.1 m cacodylate buffer, pH 7.4 (EMS, Hatfield, PA, USA).…”

Section: Methodsmentioning

confidence: 99%

Effect of lead on the physiological, biochemical and ultrastructural properties of Leucaena leucocephala

et al. 2019

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Heavy metals are characterised by a relatively high density and cause genotoxic, cytotoxic and mutagenic effects on plants, animals and humans. Lead (Pb) is one of the heavy metals that causes toxicity to plants and animals.• This experiment was conducted using a hydroponic technique to study the effects of Pb(NO 3 ) 2 on physiological, biochemical and ultrastructural characteristics in Leucaena leucocephala seedlings. Plants were grown in a growth chamber for 21 days in Hoagland's solution supplemented with 0 (control), 25, 50, 100, 300, 500 and 700 µM Pb(NO 3 ) 2 .• Shoot heights as well as root lengths decreased significantly in Pb-treated plants with 300, 500 and 700 µM. In Pb-treated plants with high Pb concentrations, photosynthesis rate (P N ), stomatal conductance (g s ) and transpiration rate (E) decreased. Total protein and carbohydrate content in Pb-treated plants with 300, 500 and 700 µM increased significantly in leaves. Moreover, in Pb-treated plants with 300, 500 and 700 µM Pb(NO 3 ) 2 , mesophyll cells had enlarged chloroplasts with disrupted thylakoid membranes associated with large starch grains. In contrast, Pb treatments with 25, 50 µM and 100 µM were not toxic to the plants. Thick sections of roots of Pb-treated plants with 300, 500 and 700 µM Pb showed distinct changes in structure of epidermal and cortical cells. Moreover, thin sections of roots of Pb-treated plants with 300, 500 and 700 µM Pb had thickened walls of xylem cells.• These results will shed more light in understanding the effects of heavy metal stress on plants.

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“…Setiap tumbuhan mengembangkan ciri adaptasi yang berbeda untuk menghadapi stress lingkungan sekitar habitatnya seperti salinitas, panas, kekeringan, genangan, logam berat dan faktor nutrient (Fayçal Boughalleb et al, 2014;Mahajan et al, 2020). Studi terhadap pengaruh stress lingkungan terhadap tumbuhan dan adaptasi yang dilakukan tumbuhan merupakan subjek penelitian yang sangat berkembang cepat di dunia (Alkhatib et al, 2018) karena berbagai stress lingkungan yang ada menjadi faktor pembatas bagi produktivitas tumbuhan (Nio Song and Banyo, 2011;Osakabe and Osakabe, 2012). Penurunan produktivitas inilah yang selanjutnya dapat menurunkan kegagalan panen dan mengancam sektor agrikultur dunia (Mahajan et al, 2020).…”

Section: Pendahuluanunclassified

“…Polutan tak hanya berupa logam berat saja, melainkan juga dapat berupa zat pemicu pertumbuhan (growth enhancer) yang konsentrasinya justru dapat menjadi inhibitor pertumbuhan. Pada akar Nicotiana tabacum yang diberi cafein sebagai growth enhancer dengan konsentrasi 5000 μM menunjukkan bahwa terjadi kerusakan mayor sel-sel korteks dan adanya penebalan dinding sel xylem (Alkhatib et al, 2018). Stress lain seperti genangan menyebabkan akar biasanya mengembangkan jaringan aerenkim seperti terlihat pada akar Ipomoea pes-caprae, Remirea maritima, Scaevola plumier (Kuster et al, 2018) dan Chrysanthemum zawadskii (Yin et al, 2012) untuk mengurangi efek hipoksia.…”

Section: Akarunclassified

Adaptasi Anatomis Tumbuhan Terhadap Perbedaan Stress Lingkungan

Akmalia¹

2021

STIGMA

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Adaptasi dilakukan oleh tumbuhan demi kelangsungan hidupnya di lingkungan yang sangat dinamis. Salah satu jenis adaptasi yang dikembangkan adalah adaptasi anatomis pada akar, batang, maupun daun. Tujuan review artikel ini yakni memberikan langkah yang dapat dilakukan manusia dalam membantu tumbuhan untuk menjaga adaptasi anatomisnya sehingga dapat mendorong pertumbuhan dan perkembangan tumbuhan di bawah stress lingkungan. Review ini didasarkan pada telaah pustaka pada artikel-artikel ilmiah yang membahas tentang adaptasi anatomis tumbuhan terhadap perbedaan stress lingkungan. Berdasarkan kajian yang dilakukan, perlu adanya upaya penelitian lebih lanjut guna menjaga tumbuhan tetap lestari di bawah stress lingkungan yang semakin menantang. Kata kunci: adaptasi; anatomi; stress lingkungan

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Physio-anatomical responses of tobacco under caffeine stress

Cited by 10 publications

References 29 publications

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco

Effect of lead on the physiological, biochemical and ultrastructural properties of Leucaena leucocephala

Adaptasi Anatomis Tumbuhan Terhadap Perbedaan Stress Lingkungan

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