Impact of estrogenic compounds on DNA integrity in human spermatozoa: Evidence for cross-linking and redox cycling activities

Bennetts, Liga; Iuliis, Geoffry N. De; Nixon, Brett; Kime, Matthew J.; Zelski, K.; McVicar, Carmel; Lewis, Sheena; Aitken, R. John

doi:10.1016/j.mrfmmm.2008.02.002

Cited by 88 publications

(67 citation statements)

References 41 publications

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“…However, other studies [15,17] showed that use of genistein at lower concentrations (0.01, 0.1, 1, 10 and 100 µmol L −1 ), produced a significant reduction in DNA damage after cells had been damaged by treatments with hydrogen peroxide. Recently, Bennetts et al [39] reported that using fresh human spermatozoa that are incubated for only 15 min, in presence of higher concentrations of genistein (31 to 500 µmol L −1 ) than those in our study, detected an increased ROS generation only with 500 µmol L −1 of genistein, but did not affect the viability or induce damage in the DNA measured by TUNEL. These authors suggested that estrogen analog compounds that did not possess vicinal hydroxyl groups, such as bisphenol A and genistein, were also fundamentally inactive in the induction of a high level of redox activity, although the latter generated a slight response at the highest dose tested [39].…”

Section: Discussioncontrasting

confidence: 54%

“…Recently, Bennetts et al [39] reported that using fresh human spermatozoa that are incubated for only 15 min, in presence of higher concentrations of genistein (31 to 500 µmol L −1 ) than those in our study, detected an increased ROS generation only with 500 µmol L −1 of genistein, but did not affect the viability or induce damage in the DNA measured by TUNEL. These authors suggested that estrogen analog compounds that did not possess vicinal hydroxyl groups, such as bisphenol A and genistein, were also fundamentally inactive in the induction of a high level of redox activity, although the latter generated a slight response at the highest dose tested [39]. motion parameters by CASA; (ii) Lipid membrane disorder status using M540 and viability using Yo-Pro1 by flow cytometry; (iii) Reactive oxygen formation by H2DCFDA staining; (iv) Chromatin condensation by PI staining; (v) DNA fragmentation by TUNEL and (vi) by the neutral comet assay.…”

Section: Discussioncontrasting

confidence: 54%

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Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 mmol L -1 ) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozenthawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

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Section: Discussioncontrasting

confidence: 54%

Section: Discussioncontrasting

confidence: 54%

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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“…Although the majority of the evidence on the effect of BPA derives from in vitro or animal studies, recent research with human tissues confirmed intense redox activity and cross--linking of the DNA in human spermatozoa [27]. In addition, epidemiologic assays have demonstrated augmented incidence of infertility treatment and an increase in the number of abnormal sperm heads among female and male workers respectively in the plastics industry [28,29].…”

Section: Introductionmentioning

confidence: 99%

BPA qualtitative and quantitative assessment associated with orthodontic bonding in vivo

et al. 2015

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OBJECTIVE: To assess the in vivo amount of BPA released from a visible light-cured orthodontic adhesive, immediately after bracket bonding. METHODS: 20 orthodontic patients were recruited after obtaining informed consent. All patients received 24 orthodontic brackets in both dental arches. In Group A (11 patients), 25 ml of tap water were used for mouth rinsing, whereas in Group B (9 patients) a simulated mouth rinse formulation was used: a mixture of 20 ml de-ionized water plus 5 ml absolute ethanol. Rinsing solutions were collected before, immediately after placing the orthodontic appliances and after washing out the oral cavity and were then stored in glass tubes. Rinsing was performed in a single phase for 60s with the entire volume of each liquid. The BPA analysis was performed by gas chromatography-mass spectrometry. RESULTS: An increase in BPA concentration immediately after the 1st post-bonding rinse was observed, for both rinsing media, which was reduced after the 2nd postbonding rinse. Water exhibited higher levels of BPA concentration than water/ethanol after 1st and 2nd post-bonding rinses. Two-way mixed Repeated Measures ANOVA showed that the primary null hypothesis declaring mean BPA concentration to be equal across rinsing medium and rinsing status was rejected (p-value <0.001). The main effects of the rinsing medium and status, as well as their interaction were found to be statistically significant (p-values 0.048, <0.001 and 0.011 respectively). SIGNIFICANCE: A significant pattern of increase of BPA concentration, followed by a decrease that reached the initial values was observed. The amount of BPA was relatively low and far below the reference limits of tolerable daily intake. Methods: 20 volunteers were recruited after obtaining informed consent. All patients received 24 orthodontic brackets in both dental arches. In Group A (11 patients), 25 ml of tap water were used for mouth rinsing, whereas in Group B (9 patients) a simulated mouth rinse formulation was used: a mixture of 20 ml de--ionized water plus 5 ml absolute ethanol. Rinsing solutions were collected before, immediately after placing the orthodontic appliances and after washing out the oral cavity and were then stored in glass tubes. Rinsing was performed in a single phase for 60 seconds with the entire volume of each liquid. The BPA analysis was performed by gas chromatography--mass spectrometry.Results: An increase in BPA concentration immediately after the 1 st post--bonding rinse was observed, for both rinsing media, which was reduced after the 2 nd post--bonding rinse. Water exhibited higher levels of BPA concentration than water/ethanol after 1st and 2nd post--bonding rinses. Two--way mixed Repeated Measures ANOVA showed that the primary null hypothesis declaring mean BPA concentration to be equal across rinsing medium and rinsing status was rejected (p--value < 0.001). The main effects of the rinsing medium and status, as well as their interaction were found to be statistically significant (p--values 0.048, <0.001 an...

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“…Although most rodent model studies show decreased sperm production and/or increased apoptosis of germ cells after exposure (27,30,33,35), others have failed to confirm these findings (40). Additionally, recent in vitro exposures of human spermatozoa to catechol estrogens (e.g., quercetin, diethylstilbestrol and pyrocatechol) indicate an impact on sperm DNA integrity through altered redox cycling, but estrogen (17␤-estradiol) and other estrogen analogues (nonylphenol and BPA) do not show this effect (41). Despite this in vitro finding using spermatozoa, how these compounds would affect spermatogenesis in vivo is unknown.…”

mentioning

confidence: 99%

Aneuploid sperm formation in rainbow trout exposed to the environmental estrogen 17α-ethynylestradiol

Brown

Schultz

Cloud

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Environmental contaminants that mimic native estrogens (i.e., environmental estrogens) are known to significantly impact a wide range of vertebrate species and have been implicated as a source for increasing human male reproductive deficiencies and diseases. Despite the widespread occurrence of environmental estrogens and recognized detrimental effects on male vertebrate reproduction, no specific mechanism has been determined indicating how reduced fertility and/or fecundity is achieved. Previous studies show that male rainbow trout, Oncorhynchus mykiss, exposed to the environmental estrogen 17␣-ethynylestradiol (EE2) before gamete formation and fertilization produce progeny with significantly reduced embryonic survival. To determine whether this observed decrease results from sperm chromosome alterations during spermatogenesis, male rainbow trout were exposed to 10 ng of EE2/l for 50 days. After exposure, semen was collected and sperm aneuploidy levels analyzed with two chromosome markers by fluorescent in situ hybridization. In vitro fertilizations were also conducted by using control and exposed sperm crossed to eggs from an unexposed female for offspring analysis. Evaluations for nucleolar organizer region number and karyotype were performed on developing embryos to determine whether sperm aneuploidy translated into embryonic aneuploidy. Results conclusively show increased aneuploid sperm formation due to EE2 exposure. Additionally, embryonic cells from propagated progeny of individuals possessing elevated sperm aneuploidy display high levels of embryonic aneuploidy. This study concludes that EE2 exposure in sexually developing male rainbow trout increases levels of aneuploid sperm, providing a mechanism for decreased embryonic survival and ultimately diminished reproductive success in EE2 exposed males.aneuploidy ͉ EE2 ͉ xenoestrogens

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Impact of estrogenic compounds on DNA integrity in human spermatozoa: Evidence for cross-linking and redox cycling activities

Cited by 88 publications

References 41 publications

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

BPA qualtitative and quantitative assessment associated with orthodontic bonding in vivo

Aneuploid sperm formation in rainbow trout exposed to the environmental estrogen 17α-ethynylestradiol

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