Impact of Elicitation on Antioxidant and Potential Antihypertensive Properties of Lentil Sprouts

Peñas, Elena; Limón, Rocio I.; Martinez-Villaluenga, Christina; Restani, Patrizia; Pihlanto, Anne; Frı́as, Juana

doi:10.1007/s11130-015-0508-3

Cited by 33 publications

(9 citation statements)

References 35 publications

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“…The TPC concentration was determined using the Folin–Ciocalteu′s phenol reagent (Peñas et al ., ). Briefly, 0.5 g of sample was extracted in 10 mL of methanol (acidified with 0.1% HCl)–water (80:20 v/v) by continuous stirring at 4 °C for 16 h. Two independent extractions were performed for each replicate.…”

Section: Methodsmentioning

confidence: 97%

Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa

Paucar-Menacho

Martínez‐Villaluenga

Dueñas

et al. 2017

Int J of Food Sci Tech

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Summary Germination has been proposed as an economic approach to improve the content of bioactive compounds in pseudocereals. In this work, response surface methodology (RSM) was employed to investigate the impact of germination conditions on the phytochemical content and antioxidant activity of quinoa. The use of desirability methodology showed that the optimum conditions to maximise the content of total phenolic content (TPC) and antioxidant activity in sprouted quinoa were 20 °C for 42 h. Sprouts produced under these conditions exhibited increases of 80% and 30% in TPC and antioxidant activity, respectively, compared to un‐germinated seeds, and contained high γ‐aminobutyric acid (GABA) concentration. The nonsignificant lack‐of‐fit and high determination coefficients obtained confirmed the suitability of the predictive models developed for TPC and antioxidant activity, whilst the one obtained for GABA was not significant (R2 < 0.75) within the conditions studied. Sprouting under optimum conditions enhanced the content of both flavonoid and nonflavonoid compounds, being the increase in flavonoids more pronounced. Kaempferol‐O‐dirhamnosyl‐galactopyranose and quercetin‐O‐glucuronide were the compounds that experienced the most noticeable increase in quinoa after germination. In conclusion, this study provides useful information on the optimum germination conditions to improve the levels of health‐promoting compounds in quinoa.

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Section: Methodsmentioning

confidence: 97%

Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa

Paucar-Menacho

Martínez‐Villaluenga

Dueñas

et al. 2017

Int J of Food Sci Tech

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“…Among biotic elicitors, polysaccharides and oligosaccharides application during germination has been widely investigated. In particular, the effect of chitosan has been investigated in several non-gramineous species such as bean [169], soybean [170,171,172], lentil [173], broccoli [174] and lettuce [175]. Additionally, yeast polysaccharide (YPS) has been regarded as an efficient biotic elicitor for stimulating secondary metabolite production.…”

Section: Factors Influencing Nutritional Quality Of Germinated Whomentioning

confidence: 99%

Sprouted Grains: A Comprehensive Review

et al. 2019

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In the last decade, there has been an increase in the use of sprouted grains in human diet and a parallel increase in the scientific literature dealing with their nutritional traits and phytochemical contents. This review examines the physiological and biochemical changes during the germination process, and the effects on final sprout composition in terms of macro- and micro-nutrients and bioactive compounds. The main factors affecting sprout composition are taken into consideration: genotype, environmental conditions experimented by the mother plant, germination conditions. In particular, the review deepens the recent knowledge on the possible elicitation factors useful for increasing the phytochemical contents. Microbiological risks and post-harvest technologies are also evaluated, and a brief summary is given of some important in vivo studies matching with the use of grain sprouts in the diet. All the species belonging to Poaceae (Gramineae) family as well as pseudocereals species are included.

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“…The non-enzymatic response involves the biosynthesis of antioxidant molecules such as phenolic compounds [15][16][17]. Previous studies have shown that the application of elicitors such as temperature, salinity, and exposure to chemical substances, including hydrogen peroxide (H 2 O 2 ) during legume sprouting, induces the accumulation of secondary metabolites with biological action [18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Improving Polyphenolic Compounds: Antioxidant Activity in Chickpea Sprouts through Elicitation with Hydrogen Peroxide

León-López

Escobar-Zúñiga

Salazar‐Salas

et al. 2020

Foods

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Elicitation appears to be a promising alternative to enhance the bioactive compound content and biological activities of legume sprouts. Multi-response optimization by response surface methodology (RSM) with desirability function (DF) was used to optimize the elicitor concentration (hydrogen peroxide (H2O2)) and germination time in order to maximize total phenolic content (TPC), total flavonoids content (TFC), and antioxidant activity (AOX) of chickpea sprouts. Chemical, antinutritional, and nutraceutical properties of optimized chickpea sprouts (OCS) were also determined. The predicted regression models developed were efficiently fitted to the experimental data. The results of the desirability function revealed that optimum attributes in chickpea sprouts can be achieved by the application of 30 mM H2O2 and 72 h of germination time, with global desirability value D = 0.893. These OCS had higher (p < 0.05) TPC (7.4%), total iso-flavonoids (16.5%), AOX (14.8%), and lower phytic acid (16.1%) and saponins (21.8%) compared to H2O2 non-treated chickpea sprouts. Optimized germination conditions slightly modified the flavonoid profile in chickpea; eight iso-flavonoids were identified in OCS, including formononetin and biochanin A, which were identified as the major compounds. Results from this study support elicitation with H2O2 as an effective approach to improve phytochemical content and antioxidant activity in chickpea sprouts.

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Impact of Elicitation on Antioxidant and Potential Antihypertensive Properties of Lentil Sprouts

Cited by 33 publications

References 35 publications

Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa

Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa

Sprouted Grains: A Comprehensive Review

Improving Polyphenolic Compounds: Antioxidant Activity in Chickpea Sprouts through Elicitation with Hydrogen Peroxide

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