Improving Polyphenolic Compounds: Antioxidant Activity in Chickpea Sprouts through Elicitation with Hydrogen Peroxide

Abstract

Elicitation appears to be a promising alternative to enhance the bioactive compound content and biological activities of legume sprouts. Multi-response optimization by response surface methodology (RSM) with desirability function (DF) was used to optimize the elicitor concentration (hydrogen peroxide (H2O2)) and germination time in order to maximize total phenolic content (TPC), total flavonoids content (TFC), and antioxidant activity (AOX) of chickpea sprouts. Chemical, antinutritional, and nutraceutical properties of optimized chickpea sprouts (OCS) were also determined. The predicted regression models developed were efficiently fitted to the experimental data. The results of the desirability function revealed that optimum attributes in chickpea sprouts can be achieved by the application of 30 mM H2O2 and 72 h of germination time, with global desirability value D = 0.893. These OCS had higher (p < 0.05) TPC (7.4%), total iso-flavonoids (16.5%), AOX (14.8%), and lower phytic acid (16.1%) and saponins (21.8%) compared to H2O2 non-treated chickpea sprouts. Optimized germination conditions slightly modified the flavonoid profile in chickpea; eight iso-flavonoids were identified in OCS, including formononetin and biochanin A, which were identified as the major compounds. Results from this study support elicitation with H2O2 as an effective approach to improve phytochemical content and antioxidant activity in chickpea sprouts.

H2O2-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production

“…This may be related to a better functioning of secondary metabolism over time, as reported in two-step in vitro production systems [42]. In this sense, the elicitation effect of exogenous H2O2 on the secondary metabolism has been reported in numerous systems [43][44][45]. On the other hand, culture in liquid medium provided higher anthocyanin concentrations-an order of magnitude higher-than those observed on solid media (Figure 2), as reported in [25,46].…”

Impact of Sprouting under Potassium Nitrate Priming on Nitrogen Assimilation and Bioactivity of Three Medicago Species

“…The bioactive compounds of Medicago have gotten a lot of interest because of their antibacterial, anti-inflammatory, anticancer, and antioxidant properties [ 7 ]. These biological actions, which include anti-inflammatory and antioxidant properties, may be due to the presence of phenolic and flavonoids chemicals, which function as free radical scavengers and/or metal chelators [ 43 ]. Caunii et al (2012) [ 44 ] found that the lucerne extract included a number of free hydroxyl groups (hydrogen donors), giving the product a significant antioxidizing effect in the human body.…”

Laser Light Treatment of Seeds for Improving the Biomass Photosynthesis, Chemical Composition and Biological Activities of Lemongrass Sprouts

“…As a consequence of increasing the antioxidant metabolites in Cymbopogon proximus sprouts, higher antioxidant activities were observed, as indicated by different antioxidant assays (FRAP, ORAC, DPPH) (Figure 1). Antioxidant activity is considered a key indicator of the nutritive value in edible plant parts [53]. In this regard, the antioxidant capacities of essential oils of Cymbopogon proximus have been previously reported [15].…”

“…assays (FRAP, ORAC, DPPH) (Figure 1). Antioxidant activity is considered a key indicator of the nutritive value in edible plant parts [53]. In this regard, the antioxidant capacities of essential oils of Cymbopogon proximus have been previously reported [15].…”