Impact of different pre-treatment strategies on the quality of fatty acid composition, tocols content and metabolic syndrome related activities of Perilla frutescens seed oil

Abstract

Perilla frutescens (Nga-Mon) is an annual herbaceous plant, reported for its antioxidant, anti-allergic, antiinflammatory and neuroprotective properties. The current study was conducted to compare the different pretreatment techniques followed by hexane extraction for perilla seed oil and its pharmaceutical values. There are no significant differences in the yield of seed oil after pre-treatments except sonication. All the pre-treatments diminish the endogenous lipase activity, peroxidation and degradation of the oil. Fatty acid content analysis revealed that the nutrient quality, with respect to fatty acid content, of perilla seed was not compromised with any of the pre-treatments of current study. The results of α-amylase, α-glucosidase and protein glycation inhibition assays suggested that tested perilla seed oils are pharmaceutical candidate for the treatment of carbohydrate related diseases, especially for diabetes. Selection of appropriate pre-treatment strategies will helps to extract the perilla seed oil without any compromise in its quality. The current study suggested that moist heat with pressure can be an appropriate pre-treatment method for perilla seed oil extraction.

Preparation and Stability Assessment of Perilla Frutescens Seed Oil Powder

“…Alpha-linolenic acid, oleic acid, linoleic acid, and palmitic acid are the major of fatty acids present in PSO [4]. The PSOP has 39.97, 26.68, 24.78, and 7.47% of linolenic acid, oleic acid, linoleic acid, and palmitic acid, respectively.…”

“…The acid and peroxide values of PSOP were determined according to Sirilun et al [4]. The acid and peroxide values were represented as mg of potassium hydroxide equivalent per gram of PSOP (mg KOH/g of PSOP), and milliequivalent of oxygen per kilogram of PSOP (mEq/Kg of PSOP), respectively.…”

“…The individual fatty acid content was measured as described in the previous report [4] and outsourced at Halal Science Center, Chulalongkorn University, Bangkok, Thailand.…”

“…The collected powders were preserved in several storage conditions until use. The percentage of yield was calculated as detailed previously [4].…”

“…The perilla seed (PS) and PS oil (PSO) are rich sources of phenolic compounds, antioxidants, and polyunsaturated fatty acids (especially, α-linolenic acid, linoleic acid, oleic acid, and palmitic acid). Several reports revealed that the PS, PSO, and perilla leaves have antioxidant, antiallergic, antiinflammatory, anticancer, neuroprotective, and α-glucosidase inhibitory properties [1][2][3][4]. PSO is commonly used to flavor the foods, and as cooking oil (particularly in Korean cuisine), and in cleansing products.…”