“…Nevertheless, for all of the samples, the uniformity coefficient, Cu, was greater than 3 (Table 4), which indicates the variability of particle sizes. This result agrees with the investigations performed by Zhang and Banks (2013), who assert that during the grinding processes, different particle sizes are achieved; these can influence certain phases of the AD process, particularly hydrolysis, when the greatest enzymatic activity during the anaerobic process occurs. Figure 3 shows that the lag phase for the latter was approximately 2.5 d (60 hours), which indicates that although the substrate predominantly contains particulate organic material, its size not only allowed adaptability of the biomass but also the microbial communities rapidly transformed the MBW (Mata et al, 2000).…”