Impact of deoxynivalenol on intestinal explants of broiler chickens: An ex vivo model to assess antimycotoxins additives

Duarte, Vinicius; Mallmann, Adriano Olnei; Liberalesso, Diogo; Simões, Cristina Tonial; Gressler, Luciane Tourem; Molossi, Franciéli Adriane; Bracarense, Ana Paula Frederico Rodrigues Loureiro; Mallmann, Carlos Augusto

doi:10.1016/j.toxicon.2021.06.016

Cited by 2 publications

(2 citation statements)

References 39 publications

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“…Duarte et al . [ 21 ] explored the protective effects of antimycotoxin additives (AMAs) against deoxinivalenol exposure on intestinal explants using broiler chicken jejuni. This study also emphasized histopathological analysis, looking at parameters such as villi number and height, crypt diameter, villi height/crypt diameter ratio, enterocyte height and nucleus size, cytoplasmic vacuolization of enterocytes, microvilli integrity, and immunostaining of caspase-3.…”

Section: Methods Using Simple Apparatusmentioning

confidence: 99%

A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science

Souza

Guedes

2023

Vet World

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Different techniques have been reported in studies of intestinal in vitro organ culture (IVOC). A robust compilation of all available methods is lacking in the literature, making it difficult to choose a method that corresponds to the study's demands. In this review, readers can assess the most available methods, allowing them to evaluate which is more suitable for their purposes and requirements. A simplified view of culturing intestinal explants is presented, highlighting the approachability of IVOC. Relevant findings from diverse veterinarian studies, where explants played a major role, as well as the technique used in each, are described to illustrate its applications. Finally, the strengths and limitations of the innovative intestinal IVOC methods are discussed. This review provides a collection of methods for intestinal explant culture and their possible applications in veterinary research. In this way, it aims to broaden access to IVOC techniques and aid decision-making regarding the best suited for a study's purposes.

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Section: Methods Using Simple Apparatusmentioning

confidence: 99%

A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science

Souza

Guedes

2023

Vet World

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“…The intestine and liver are high protein turnover organs and are the main targets of DON cytotoxicity [ 5 , 7 ]. DON also damages the epithelial integrity and reduces the repair and replacement rates of epithelial cells through its negative effects on the intestinal barrier [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function

Kang,

Lee

2024

J Animal Sci Biotechnol

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Background Deoxynivalenol (DON) is a mycotoxin that has received recognition worldwide because of its ability to cause growth delay, nutrient malabsorption, weight loss, emesis, and a reduction of feed intake in livestock. Since DON-contaminated feedstuff is absorbed in the gastrointestinal tract, we used chicken organoids to assess the DON-induced dysfunction of the small intestine. Results We established a culture system using chicken organoids and characterized the organoids at passages 1 and 10. We confirmed the mRNA expression levels of various cell markers in the organoids, such as KI67, leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5), mucin 2 (MUC2), chromogranin A (CHGA), cytokeratin 19 (CK19), lysozyme (LYZ), and microtubule-associated doublecortin-like kinase 1 (DCLK1), and compared the results to those of the small intestine. Our results showed that the organoids displayed functional similarities in permeability compared to the small intestine. DON damaged the tight junctions of the organoids, which resulted in increased permeability. Conclusions Our organoid culture displayed topological, genetic, and functional similarities with the small intestine cells. Based on these similarities, we confirmed that DON causes small intestine dysfunction. Chicken organoids offer a practical model for the research of harmful substances.

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Impact of deoxynivalenol on intestinal explants of broiler chickens: An ex vivo model to assess antimycotoxins additives

Cited by 2 publications

References 39 publications

A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science

A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science

Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function

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