In livestock species, the small intestine plays a crucial role as the site for food digestion and nutrient absorption, and these functions are carried out by the epithelial cells forming a single layer on the surface of the intestine. Research on these animals has been essential to improve their health and productivity, and to achieve this, immortalized cell lines and primary cell cultures have been employed as in vitro models. However, these models had limitations as they could not completely replicate the functionality of real tissues. Recently, methods for culturing intestinal organoids from various livestock species, including pigs, cattle, chickens, rabbits, and horses, have been developed. Intestinal organoids address several drawbacks associated with traditional in vitro models, opening up new possibilities for studying intestinal epithelia. This paper explores the development of intestinal organoids in various livestock species and the research based on these models.