Impact of colostomy on intestinal microflora and bacterial translocation in young rats fed with heat-killed Lactobacillus acidophilus strain LB

Abstract

A rat animal model of left colostomy was found to significantly impair the growth curve of rats. Assessment of the intestinal flora showed that colostomy mostly affects the cecal but not colonic microflora. Generally, the number of enterococci was increased in both ileum and cecum; cecal lactobacilli also rose, accounting for a promotion of lactic acid bacteria in colostomised rats. No significant differences between colostomised, laparotomised and control rats could be observed for the translocation of intestinal bacteria to internal organs of rats (i.e. spleen, kidneys, lungs or liver), whatever their diet. Heat-killed Lactobacillus acidophilus strain LB administration (dead probiotic bacteria) tended to exhibit a stimulatory effect on bifidobacteria, probably affecting the culture-medium fermentation substances included in the pharmaceutical product. This effect was abolished by laparotomy and colostomy. A trend towards a probiotic-like effect, not susceptible to colostomy, was also witnessed as counts of lactobacilli tended to increase in both cecum and colon of all animals fed with L. acidophilus LB.

Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to caco-2 cells

“…Most of the probiotic bacteria are lactic acid bacteria which are useful in the treatment of dysfunctions that disturb intestinal microflora and abnormal gut permeability (Lee and Salminen 1995;Ebringer et al 2008;Koninkx and Malago 2008;Rigon-Zimmer et al 2008). Successful probiotic bacteria are usually able to colonize the intestine, at least temporarily, by adhering to the intestinal mucosa.…”

Lactobacillus isolates from weaned piglets’ mucosa with inhibitory activity against common porcine pathogens

“…Among the probiotics for animal feed authorized in the EU (Community Register of Feed Additives 2003), there are at the moment only a few Lactobacillus representatives which have been authorized under the new regulations (Regulation EC no. 1831see Community Register 2003): two strains of L. acidophilus (see, e.g., Walencka et al 2008;Rigon-Zimmer et al 2008), one strain belonging to L. farciminis. Most of the probiotic feed products on the market contain Bacillus licheniformis and B. subtilis strains (Lomáková et al 2006;Trebichavský and Šplíchal 2006), although the members of Bacillus genus are not typical indigenous organisms, and Enterococcus faecium -the species belonging to the most robust genus among LAB (see, e.g., Marciňáková et al 2006).…”

Effects of a Formula Containing Two Types of Prebiotics, Bifidogenic Growth Stimulator and Galacto-oligosaccharide, and Fermented Milk Products on Intestinal Microbiota and Antibody Response to Influenza Vaccine in Elderly Patients: A Randomized Controlled Trial

“…In fact, BGS intake was effective to increase the detection rate of fecal bifidobacteria and improved the fecal properties in enterally fed elderly patients [ 11 ]. Although probiotics are generally defined as live microbial feed supplements, heat-killed Lactobacillus administration also exhibits a stimulatory effect on bifidobacteria [ 27 ]. Therefore, the three nutritional ingredients of the study formula, namely, BGS, GOS, and heat-treated milk products fermented by L. delbrueckii subsp.…”