Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to caco-2 cells

Ostad, Seyed Nasser; Salarian, Ali; Ghahramani, Mohammad-Hosein; Fazeli, Mohammad Reza; Samadi, Nasrin; Jamalifar, Hossein

doi:10.1007/s12223-009-0024-7

Cited by 40 publications

(26 citation statements)

References 23 publications

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“…farciminis 3699 also correlated with an increase of their potential for exclusion against C. jejuni CIP 70.2 T . These data are in accordance with the study of Ostad et al (2009), who demonstrated that the inhibition of attachment of the pathogenic bacteria by inactivated cells of faecal Lb. acidophilus was similar for live and heat-inactivated forms.…”

Section: Discussionsupporting

confidence: 93%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

112

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Bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. In the present study, two probiotic strains, Lactobacillus rhamnosus CNCM-I-3698 and Lactobacillus farciminis CNCM-I-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference Lactobacillus strains (Lb. rhamnosus GG and Lb. farciminis CIP 103136). The autoaggregation potential of both forms was higher than that of reference strains and twice that of pathogenic strains. The coaggregation potential of these two beneficial micro-organisms was evaluated against several pathogenic agents that threaten the global safety of the feed/food chain: Escherichia coli, Salmonella spp., Campylobacter spp. and Listeria monocytogenes. The strongest coaggregative interactions were demonstrated with Campylobacter spp. by a coaggregation test, confirmed by electron microscopic examination for the two forms. Viable forms were investigated for the nature of the bacterial cell-surface molecules involved, by sugar reversal tests and chemical and enzymic pretreatments. The results suggest that the coaggregation between both probiotic strains and C. jejuni CIP 70.2 T is mediated by a carbohydrate-lectin interaction. The autoaggregation potential of the two probiotics decreased upon exposure to proteinase, SDS or LiCl, showing that proteinaceous components on the surface of the two lactobacilli play an important role in this interaction. Adhesion abilities of both Lactobacillus strains were also demonstrated at significant levels on Caco-2 cells, mucin and extracellular matrix material. Both viable and heat-killed forms of the two probiotic lactobacilli inhibited the attachment of C. jejuni CIP 70.2 T to mucin. In conclusion, in vitro assays showed that Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, as viable or heat-killed forms, are adherent to different intestinal matrix models and are highly aggregative in vitro with pathogens, especially Campylobacter spp., the most commonly reported zoonotic agent in the European Union. This study supports the need for further in vivo investigations to demonstrate the potential food safety benefits of Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, live or heat-killed, in the global feed/food chain.

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Section: Discussionsupporting

confidence: 93%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

112

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“…Moreover, heat inactivation of E. faecalis aimed at preparation of experimental inoculum without loss of virulence or number of bacteria and at impeding the overexpansion that a live bacterial population normally develops. Indeed, bacterial adherence to cells does not seem to be inhibited by heat inactivation [32]. Although heat killing at 90°C would be expected to denaturate all or most of the protein virulence factors, other components such as LTA do not seem to be affected.…”

Section: Discussionmentioning

confidence: 97%

Enterococcus faecalis affects the proliferation and differentiation of ovine osteoblast-like cells

et al. 2011

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Enterococcus faecalis (E. faecalis) is a Gram-positive bacterium, mostly recovered from root-filled teeth with persistent periapical lesions. Bacterial contamination of root canals inevitably results in interaction between E. faecalis and periapical tissues during the dynamic process of periapical inflammation. This study investigated the impact of heat-inactivated endodontic E. faecalis on the proliferation and the differentiation of ovine osteoblast-like cells, in an attempt to elucidate its putative enhanced pathogenicity mechanisms. Therefore, two different concentrations of a heat-inactivated endodontic E. faecalis isolate (2 × 10(6) or 2 × 10(8) CFU/ml) were incubated with ovine osteoblast-like cells for 7 and 14 days, respectively. Cells without antigen served as control. The effects of antigen on cell growth were evaluated by a proliferation assay (EZ4U). Furthermore, the assessment of alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin (OCN) gene expression through quantitative real-time PCR determined the degree of osteogenic cell differentiation. Scanning electron microscopy (SEM) was also performed to detect alterations in cell morphology. Interestingly, although highly concentrated E. faecalis increased cellular reproduction after 14 days, ALP activity and OCN gene expression decreased in an antigen concentration-dependent and incubation time-independent way. SEM images revealed E. faecalis adhesion on cells, a fact that might contribute to its virulence. These results suggest that E. faecalis stimulated cell multiplication, whereas it likely restrained cell differentiation of ovine osteoblast-like cells. In conclusion, the presence of E. faecalis in root canals may negatively affect periapical new bone formation, and thus, the healing of periapical lesions.

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“…viable nonpathogenic microorganisms exhibiting beneficial health effects when consumed in adequate amount, seems to be a perspective way for maintenance of balanced microflora (Schrezenmeier and de Vrese 2001). Probiotics are believed to trigger innate and adaptive immune responses and suppress colonization of the intestine by pathogenic bacteria by competing with them for a limited number of receptors present on the surface epithelium (Ostad et al 2009). They also influence enhancement of mucosal barrier function, increase production of secretory IgA (Vanciková et al 2003) and elicit production of shortchain fatty acids with health benefits related to colitis and cancer prevention (Fedorak and Madsen 2004;Kokesova et al 2006).…”

Section: Abbreviationsmentioning

confidence: 99%

Probiotic Lactobacillus strains: in vitro and in vivo studies

et al. 2009

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Three Lactobacillus strains (LOCK 0900, LOCK 0908, LOCK 0919) out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb/c mice affected cytokine T(H)1-T(H)2 balance toward nonallergic T(H)1 response. Spleen cells, isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains, produced significantly higher amounts of anti-allergic tumor necrosis factor- and interferon-gamma than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood, liver and spleen; a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900, LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and/or therapy of allergic diseases.

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Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to caco-2 cells

Cited by 40 publications

References 23 publications

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Enterococcus faecalis affects the proliferation and differentiation of ovine osteoblast-like cells

Probiotic Lactobacillus strains: in vitro and in vivo studies

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