Impact of Colony Morphologies and Disinfection on Biological Clogging in Porous Media

Dupin, Hubert J.; McCarty, Perry L.

doi:10.1021/es990452f

Cited by 92 publications

(62 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When the pH was lowered, the biomass concentration first decreased and thereafter increased before returning to its steady-state value. The increased suspended biomass concentrations after the pH drops might be due to sloughing of the wall-growth, which is known to occur under harsh conditions (Dupin and McCarty, 2000).…”

Section: Bioreactor Functional Stabilitymentioning

confidence: 99%

Strain stability in biological systems treating recalcitrant organic compounds

Emanuelsson

Baptista

Mantalaris

et al. 2005

Biotech & Bioengineering

View full text Add to dashboard Cite

The availability of molecular probing technology in recent years has facilitated investigation of microbial community composition during bio-treatment of organic wastes. Particularly, it has allowed the study of microbial culture stability and correlation between stability and treatment performance. However, most studies to date have only addressed mixed cultures and there is limited information regarding single strain stability. Here we have investigated the microbial community dynamics in two bioreactors, each inoculated with a pure bacterial strain capable of degrading a recalcitrant substrate, namely Xanthobacter aut. GJ10 degrading 1,2-dichloroethane (DCE) and Burkholderia sp. JS150 degrading monochlorobenzene (MCB). Universal and strain specific 16S rRNA oligonucleotide probes were designed and used to follow strain stability. The bioreactor fed with DCE was functionally stable and the percentage of GJ10 cells in the community remained high (around 95% of total cells) throughout, even after introduction of foreign microorganisms. The bioreactor fed with MCB was also functionally stable, but in contrast to the DCE bioreactor, probing results revealed the disappearance of strain JS150 from the bioreactor within a week. The difference in behavior between the two systems is attributed to the specific pathway required to degrade DCE.

show abstract

Section: Bioreactor Functional Stabilitymentioning

confidence: 99%

Strain stability in biological systems treating recalcitrant organic compounds

Emanuelsson

Baptista

Mantalaris

et al. 2005

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…As suggested, biofilms are likely to consume more substrate than aggregates for a given permeability reduction. It has been observed [Dupin and McCarty, 2000] that biofilm and aggregates develop simultaneously from mixed culture. Filamentous growth has also been observed under acidic conditions [Dupin and McCarty, 1999].…”

Section: Model Limitations and Further Researchmentioning

confidence: 99%

Simulations of two‐dimensional modeling of biomass aggregate growth in network models

Dupin¹,

Kitanidis²,

McCarty³

2001

Water Resources Research

Self Cite

View full text Add to dashboard Cite

Abstract. We investigate the mechanisms by which microorganism that grow in the form of aggregates impact the permeability and the transport properties of porous media modeled as two-dimensional networks. In a companion paper [Dupin et al., this issue] we present a model of processes in a single channel. In this paper, we describe how to assemble channels into networks. Simple networks are investigated to identify phenomena of interest: four channels of different width operating in parallel to study the effect of local heterogeneity; a periodic network to quantify the effects of distance from the injection point on clogging and substrate utilization; and square lattice 5 x 5 random width networks. Although square lattice random width networks are deemed better approximations of porous media, the simpler networks exhibit all the phenomena of interest, with the added advantage of these phenomena being decoupled. Results of numerical simulations for different network types under various boundary conditions show that aggregates have a far greater potential than biofilms to clog a porous medium.

show abstract

“…Microscopic observations of the distribution of microbial biomass in sediment and soil pores, for example, via scanning electron or confocal laser microscopy, show unequivocally that the biomass is distributed very irregularly in natural porous media (Baveye et al, 1998;Dupin and McCarty, 2000;Vandevivere and Baveye, 1992). This suggests the need to adopt modeling techniques that can accommodate discontinuous biomass structures (e.g., Valocchi, 1989, 1991;Dupin et al, 2001a,b;Hermanowicz, 1999;Vandevivere et al, 1995;Wood and Whitaker, 1999).…”

Section: Introductionmentioning

confidence: 99%

Computational pore network modeling of the influence of biofilm permeability on bioclogging in porous media

Thullner

Baveye

2007

Biotech & Bioengineering

101

132

View full text Add to dashboard Cite

For many years, controversy has surrounded the use of biofilm models to describe the distribution of microbial biomass in natural or artificial porous media. This use is often advocated on the basis of the relative mathematical simplicity of the biofilm concept, and of the widespread availability of analytical solutions or numerical implementations. However, microscopic observations consistently point to a patchy, rather than homogeneous, distribution of microorganisms at the pore scale in many porous media of interest, even under conditions of severe bioclogging. Also, bioclogging models involving biofilms tend to underpredict the extent of permeability reductions in all be the coarse-textured materials. In this context, computer simulations described in the present article show that some of the limitations of biofilm models to describe the bioclogging of porous media are linked to the common constitutive assumption that biofilms are impermeable, that is, that nutrient transport occurs through the biofilms only by molecular diffusion. When this restriction is alleviated and liquid flow is allowed in the biofilms, the level of bioclogging achievable by a given biomass is very significantly increased and is comparable to that observed in experiments. In addition, the distribution of microorganisms becomes patchy and exhibits a self-organized periodic pattern with pores either entirely filled with biomass or without any biomass at all, again similar to published microscopic observations. These results suggest that biofilm models should not be ruled out a priori for the quantitative description of bioclogging in porous media, as long as biofilms are allowed to be permeable.

show abstract

Impact of Colony Morphologies and Disinfection on Biological Clogging in Porous Media

Cited by 92 publications

References 23 publications

Strain stability in biological systems treating recalcitrant organic compounds

Strain stability in biological systems treating recalcitrant organic compounds

Simulations of two‐dimensional modeling of biomass aggregate growth in network models

Computational pore network modeling of the influence of biofilm permeability on bioclogging in porous media

Contact Info

Product

Resources

About