Strain stability in biological systems treating recalcitrant organic compounds

Emanuelsson, Emma Anna Carolina; Baptista, Inês; Mantalaris, A.; Livingston, Andrew G.

doi:10.1002/bit.20620

Cited by 17 publications

(20 citation statements)

References 41 publications

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“…This strain has been studied in several bioreactor configurations for the treatment of MCB contaminated streams (Ferreira Jorge and Livingston, 2000;Haigler et al, 1992;Oliveira and Livingston, 2003). However, the latest study reported its disappearance from a functionally stable bioreactor (Emanuelsson et al, 2005). Similar cases of species out-competition and succession during biodegradation processes have been reported (MassolDeya et al, 1997;Springael et al, 2002;Von Canstein et al, 2001).…”

Section: Introductionmentioning

confidence: 82%

“…The MCB concentration in the biomedium and the biomass concentration were analysed as previously described (Emanuelsson et al, 2005). Chloride concentration was analysed by ion chromatography (Dionex DX 120 with an IonPAC AS114 4 mm Â 250 mm column; Camberley, UK).…”

Section: Methodsmentioning

confidence: 99%

“…The CSTR degrading MCB was run as previously described (Emanuelsson et al, 2005). In short, a continuously stirred bioreactor with a working volume of 2.3 L was inoculated with strain JS150 and fed a synthetic solution containing MCB at 0.3 g L À1 , supplied at a flow rate of 0.112 L h…”

Section: Cstr Operation and Growth Kineticsmentioning

confidence: 99%

“…Bacterial samples collected from the initial MCB bioreactor (reported in Emanuelsson et al, 2005) were analysed. Bacterial samples were washed in phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde as described by Amann et al (1990).…”

Section: Sample Collection and In Situ Hybridisationmentioning

confidence: 99%

“…Recently, we have applied fluorescence in situ hybridisation (FISH) to monitor the rapid disappearance of a specific strain from a bioreactor degrading MCB (Emanuelsson et al, 2005). In the present work, species succession was investigated and a novel chlorobenzene degrader belonging to the Pandoraea genus was isolated.…”

Section: Introductionmentioning

confidence: 99%

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Evidence of species succession during chlorobenzene biodegradation

Baptista

Zhou

Emanuelsson

et al. 2007

Biotech & Bioengineering

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We have previously reported the disappearance of a specific strain degrading chlorobenzene from a functionally stable bioreactor. In the present work, we investigated this species succession and isolated a new dominant strain, identified as Pandoraea pnomenusa sp. strain MCB032. A specific 16S rRNA-targeted oligonucleotide probe was designed and validated to identify strain MCB032 using fluorescence in situ hybridisation (FISH). The results confirmed the presence of strain MCB032 in samples collected over time, and showed that it was primarily located within the biofilm. Denaturing gradient gel electrophoresis (DGGE) provided evidence that the species succession occurred early in the operating period. The application of these biomolecular tools highlighted the remarkable stability of this new strain during the 15 months of reactor operation. The succession was attributed to the competitive kinetic behaviour of strain MCB032, which exhibited faster growth (micro(max) = 0.34 h(-1)) and higher substrate affinity (K(s) = 0.35 mg L(-1)) than strain JS150. Finally, this study contributed to the characterisation of the recently established Pandoraea genus, an emerging group in the biodegradation field.

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Section: Introductionmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Cstr Operation and Growth Kineticsmentioning

confidence: 99%

Section: Sample Collection and In Situ Hybridisationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evidence of species succession during chlorobenzene biodegradation

Baptista

Zhou

Emanuelsson

et al. 2007

Biotech & Bioengineering

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Microbiology for chemical engineers—from macro to micro scale

Emanuelsson

Patterson

et al. 2007

Asia-Pacific J Chem Eng

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Recent developments in microbial techniques (such as PCR, GE, FISH) have allowed researchers to detect, identify and quantify microorganisms without the limitation of culture-dependent methods. This has given both engineers and scientists a more fundamental understanding about systems containing microorganisms. These techniques can be used to monitor bacteria in wastewater treatment systems, soil and sea, industrial fermentation, food technology, and improve floccability, etc. However, despite these techniques being readily available and relatively cheap, they are not widely used by engineers. Hence, the aim of this paper is to introduce these techniques, and their applications, to chemical engineers. Two different studies related to industrial wastewater treatment, but applicable to general microorganism systems, will be presented: (1) microbial stability of pure cultures, and (2) bioreactor population shifts during alternating operational conditions. In (1), two bioreactors, inoculated with two different pure cultures, (A) Xanthobacter aut GJ10 and (B) Bulkholderia sp JS150, degrading 1,2-dichloroethane (DCE) and monochlorobenzene (MCB), respectively, were followed over time (Emanuelsson et al ., 2005). Specific and universal 16S rRNA oligonucleotide probes were used to identify the bacteria. It was found that bioreactor (A) remained pure for 290 days, whereas bioreactor (B) became contaminated within one week. The difference in behaviour is attributed to the pathway required to degrade DCE. In (2), the stability of a bacterial strain, which was isolated on the basis of its capability to degrade 2-fluorobenzoate from contaminated soil, in three different, up-flow fixed bed reactors operated under shock loads and starvation periods, was followed by denaturing gradient gel electrophoresis (DGGE) (Emanuelsson et al ., 2006). All bioreactors were rapidly colonised by different bacteria; however, the communities remained fairly stable over time, and shifts in bacterial populations were mainly found during the starvation periods.

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Treatment of halogenated organic compounds and monitoring of microbial dynamics in up‐flow fixed bed reactors under sequentially alternating pollutant scenarios

Emanuelsson¹,

Osuna²,

Sipma³

et al. 2008

Biotech & Bioengineering

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Two up-flow fixed bed reactors (UFBR) were operated for 8 months treating a model synthetic wastewater containing 2-fluorobenzoate (2-FB) and dichloromethane (DCM). The stability of the reactors under dynamic conditions, that is, sequentially alternating pollutants (SAP), shock loads, and starvation periods was assessed. Two support materials were used: expanded clay (EC) that does not adsorb 2-FB or DCM, and granular-activated carbon (GAC) that adsorbs 180 mg g(-1) of 2-FB and 390 mg g(-1) of DCM. The reactors were inoculated with a 2-FB-degrading strain (FB2) and a DCM degrader (TM1). 2-FB was fed at organic loads ranging from 0 to 800 mg L(-1) d(-1), while DCM was fed at 0-250 mg L(-1) d(-1). 2-FB or DCM were never detected at the outlet of the GAC reactor, while in the EC reactor outlet small amounts were observed. Nevertheless, the highest biological elimination capacity was observed in the EC reactor (over 700 mg L(-1) d(-1) of 2-FB). DGGE analysis revealed a fairly stable bacterial community with the largest shifts occurring during starvation periods and changes in feed composition. Several bacterial strains isolated from the reactors showed capacity for 2-FB degradation, while only strain TM1 degraded DCM.

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Strain stability in biological systems treating recalcitrant organic compounds

Cited by 17 publications

References 41 publications

Evidence of species succession during chlorobenzene biodegradation

Evidence of species succession during chlorobenzene biodegradation

Microbiology for chemical engineers—from macro to micro scale

Treatment of halogenated organic compounds and monitoring of microbial dynamics in up‐flow fixed bed reactors under sequentially alternating pollutant scenarios

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