IMPACT OF CERTAIN PLANT EXTRACTS ON ENZYMES ACTIVITY OF TOMATO LEAFMINER Tuta absoluta.

Abstract

The experiments were conducted in the laboratory Plant Protection Research Institute Dokki-Giza, to study the effect of Anethum groveolenus Dill, Ambrosia maritime Damaseia extract, Cumminum cyminum Cumin; Syzyguim aromaticum Clove and Allium maritime Garlic on activity of some enzymes in(Tuta absoluta Meyrick) on tomato plants were investigated. After the tested compounds application, the results revealed that these compounds had various effects on the activity alkaline phosphatases, α and β esterases and carbohydrates hydrolyzing enzymes (trehalase, invertase and amylase). The enzyme activity reduced or increased significantly. Activity of alkaline phosphatases, α and β esterases were higher in Damasiea on T. absoluta, in comparison to control. The percentage of increase of alkaline enzymes activity were +22.28 and +17.38 and +13.34 % in T. absoluta respectively. Also, in cumin and garlic extracts treatments. Generally, in case of T. absoluta treatments, the results of damasiea extract indicated high increase (+40.1% in comparison to control) in activity of beta-esterases and invertase enzymes but high decrease (+45.9%), in alpha-esterases activity only. The results indicated that the damasiea extract had direct effects on the metabolism in the body Tuta absoluta.

PHYSIOLOGICAL CHANGES AND CHEMICAL COMPONENTS OF HAEMOLYMPH OF THE MEDFLY Ceratitis capitata WIED. THIRD INSTAR LARVAE AFFECTED BY THREE DIFFERENT PREPARED BOTANICAL EXTRACTS

“…The obtained results are in harmony with those reported byYapi et al (2007) who purified a beta-glucosidase from the digestive juice of the palm weevil Rhynchophorus palmarum L. larvae; indicated the presence of alpha and betaglucosidaes and alpha and beta galactosidases in digestive system and haemolymph of R. ferrugineus and the mean specific activity of β-glucosidase was 0.067 Mmol/min/mg protein Taha et al (2015). mentioned the effect of certain extracts on enzymes activity of tomato leafminer.…”

Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae)

“…However, rhodamine B, basil EO, mixed rose bengal and basil EO (mixture A), and mixed rhodamine B and basil EO (mixture C) result in a significant reduction in trehalase activity. In the same context, trehalase in the tomato leafminer Tuta absoluta was the most affected enzyme with a significantly high level after treatment with clove EO (Taha et al 2015). The reduction in trehalase activity observed in adult aphids treated with rhodamine B during exposure to sunlight indicates that the generated reactive oxygen species (ROS) induce further damage to the enzyme.…”