Impact of blue LED irradiation on proliferation and gene expression of cultured human keratinocytes

Becker, A.; Sticht, Carsten; Dweep, Harsh; Abeelen, F. A. van; Gretz, Norbert; Oversluizen, G.

doi:10.1117/12.2083010

Cited by 9 publications

(18 citation statements)

References 21 publications

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“…Proliferation of HaCaT cells after PBM with blue light revealed the well-known biphasic response curve, with a slight increase of proliferation for 7.5 min and an anti-proliferative effect for 15 min (20.7 J/cm 2 ). Longer irradiation times (up to 120 min, 165.6 J/cm2) did not result in a higher anti- proliferative effect 22 .…”

mentioning

confidence: 79%

Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light

Becker

Klapczynski

Kuch

et al. 2016

Sci Rep

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Photobiomodulation (PBM) with blue light induces a biphasic dose response curve in proliferation of immortalized human keratinocytes (HaCaT), with a maximum anti-proliferative effect reached with 30min (41.4 J/cm2). The aim of this study was to test the photobiomodulatory effect of 41.4 J/cm2 blue light irradiation on ROS production, apoptosis and gene expression at different time points after irradiation of HaCaT cells in vitro and assess its safety. ROS concentration was increased 30 min after irradiation. However, already 1 h after irradiation, cells were able to reduce ROS and balance the concentration to a normal level. The sudden increase in ROS did not damage the cells, which was demonstrated with FACS analysis where HaCaT cells did not show any sign of apoptosis after blue light irradiation. Furthermore, a time course could be seen in gene expression analysis after blue light, with an early response of stimulated genes already 1 h after blue light irradiation, leading to the discovery of the aryl hydrocarbon receptor as possible target for blue light irradiation.

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mentioning

confidence: 79%

Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light

Becker

Klapczynski

Kuch

et al. 2016

Sci Rep

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“…Currently, multiple cellular readouts have been evaluated where the impact is assessed via cell viability , proliferation , differentiation , morphology and apoptosis . However, more specific methods are now needed to elucidate the stimulated pathways, including the assessment of specific gene expression in particular skin cell subpopulations for selected applications .…”

Section: Multiple Downstream Biomolecular Reactions Explaining Physiomentioning

confidence: 99%

Photobiomodulation devices for hair regrowth and wound healing: a therapy full of promise but a literature full of confusion

Mignon

Botchkareva

Uzunbajakava

et al. 2016

Experimental Dermatology

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Photobiomodulation is reported to positively influence hair regrowth, wound healing, skin rejuvenation and psoriasis. Despite rapid translation of this science to commercial therapeutic solutions, significant gaps in our understanding of the underlying processes remain. The aim of this review was to seek greater clarity and rationality specifically for the selection of optical parameters for studies on hair regrowth and wound healing. Our investigation of 90 reports published between 1985 and 2015 revealed major inconsistencies in optical parameters selected for clinical applications. Moreover, poorly understood photoreceptors expressed in skin such as cytochrome c oxidase, cryptochromes, opsins etc. may trigger different molecular mechanisms. All this could explain the plethora of reported physiological effects of light. To derive parameters for optimal clinical efficacy of photobiomodulation, we recommend a more rational approach to underpin clinical studies, with research on molecular targets and pathways using well-defined biological model systems to enable translation of optical parameters from in vitro to in vivo. Furthermore, special attention needs to be paid when conducting studies for hair regrowth, aiming for double-blind, placebocontrolled randomized clinical trials as the gold standard for quantifying hair growth.

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“…While perhaps the vast majority of applications focuses on red and NIR wavelengths, UV‐free blue light, corresponding to the spectral range of both violet (400‐450 nm) and blue (450‐495 nm), has been attracting increasing attention. A solid body of scientific data has been accumulated in the last decade, stemming from in vitro experiments on human cutaneous cells performed using well‐defined irradiation parameters . The current evidence attributes the high therapeutic potential of UV‐free 453 nm blue light to its antiproliferative capacity on human endothelial cells, keratinocytes, and fibroblasts, often ascribed to initiation of cell differentiation triggered by the release of nitric oxide (NO) from nitrosated proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, while fluences up to 500 J/cm 2 were noncytotoxic when applied to in vitro cell cultures of human endothelial cells and keratinocytes, a dose‐dependent depletion of T lymphocytes was observed, providing initial evidence for an anti‐inflammatory effect of blue light. Additional evidence in this respect came from in vitro studies using HaCaT cells, where gene expression analysis showed downregulated pathways implicated in inflammatory responses, and using dendritic cells, where a reduced ability to release pro‐inflammatory cytokines was shown . At the clinical level, the recently reported positive impact of blue light on alleviating symptoms of psoriasis vulgaris (PV) and atopic dermatitis (AD), chronic inflammatory skin diseases characterized by hyperproliferation and inflammatory infiltrates, holds promise for its therapeutic potential …”

Section: Introductionmentioning

confidence: 99%

Effects of blue light on inflammation and skin barrier recovery following acute perturbation. Pilot study results in healthy human subjects

Falcone

Uzunbajakava

Abeelen

et al. 2017

Photoderm Photoimm Photomed

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Impact of blue LED irradiation on proliferation and gene expression of cultured human keratinocytes

Cited by 9 publications

References 21 publications

Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light

Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light

Photobiomodulation devices for hair regrowth and wound healing: a therapy full of promise but a literature full of confusion

Effects of blue light on inflammation and skin barrier recovery following acute perturbation. Pilot study results in healthy human subjects

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