Impact of blood manufacturing and donor characteristics on membrane water permeability and in vitro quality parameters during hypothermic storage of red blood cells

Alshalani, Abdulrahman; Howell, Anita; Acker, Jason P.

doi:10.1016/j.cryobiol.2017.12.008

Cited by 15 publications

(5 citation statements)

References 30 publications

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“…Decreased ESC is interpreted as energy deficiency in the red blood cells. Elevated ESC is considered an indicator of membrane damage and cellular disorganization (Kopytova, 2006;Grzeskowiak et al, 2018;Vinogradov et al, 2017;Alshalani et al, 2018). According to the results of the ESC studies in women of reproductive age with IDA, this parameter is increased compared to the control group (Table 2).…”

Section: Resultsmentioning

confidence: 99%

The State of the Red Blood Cell Membrane in Iron Deficiency Anaemia in Women of Reproductive Age

Arystan¹,

Muldaeva²,

Haydargalieva³

et al. 2019

PTQ

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The problem of iron deficiency is urgent in many countries, regardless of the socio-economic standards of living. Among biomedical problems, the study of biochemical blood parameters is of paramount importance. A great interest in various aspects of this issue is not accidental, since the transport function of the blood largely depends on it and, consequently, the efficient delivery of oxygen, glucose, amino acids, fats, vitamins, mineral salts, hormones, mediators, antibodies and metabolites to the micro-zones in the tissues. The purpose of this article is to study the hematological status and the state of the red blood cell membrane in women of reproductive age suffering from iron deficiency anemia (IDA). Iron deficiency anemia (IDA) is a combination of clinical and hematological symptoms characterized by impaired hemoglobin formation due to iron deficiency in serum and bone marrow, as well as the development of trophic disorders in the organs and tissues. During the study, a complete blood count was performed using an enzyme-linked immunosorbent assay, using the kits produced by JSC Vector-Best, and the Kamyshnikov method. Based on the results of the study of the erythrocyte sorption capacity (ESC) in women of reproductive age with IDA, it was established that this parameter is 1.2 times higher than that in the control group. The development of anemia is associated with significant changes in the osmotic stability of the erythrocytes, which results in an increase in the proportion of both unstable and high-resistance erythrocytes. The scientific novelty of the article is that the authors established the key parameters for iron deficiency anemia detection.

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Section: Resultsmentioning

confidence: 99%

The State of the Red Blood Cell Membrane in Iron Deficiency Anaemia in Women of Reproductive Age

Arystan¹,

Muldaeva²,

Haydargalieva³

et al. 2019

PTQ

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“…17 Increased MOF has also been documented in stored human WB 37 and PRBCs, 12,38 and stored canine PRBCs. 31 Changes to MOF are not a uniformly reported feature of storage lesion, with some human blood storage studies [39][40][41] and a recent study of stored FWB collected by a novel closed collection system 3 demonstrating no statistically significant MOF increase.…”

Section: Discussionmentioning

confidence: 99%

Effect of microaggregate filter passage on feline whole blood stored for 35 days

Morse

Mooney

2021

Journal of Feline Medicine and Surgery

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Objectives The aim of this study was to compare the characteristics of fresh and stored feline red blood cells (RBCs) after passage through an 18 μm microaggregate filter. Methods Nine cats were recruited for a single blood donation using an open collection system. A simulated transfusion using a syringe driver and microaggregate filter was performed over 2 h with half the blood on the day of donation and the other half after 35 days of storage. Differences in haematological parameters, haemolysis percentage and osmotic fragility (OF) were compared on the day of donation pre-filter passage (D0–) vs day of donation post-filter (D0+) or day 35 storage pre-filter (D35–) and post-filter (D35+). Blood was cultured at D0+ and D35+. Results There were no statistically significant differences in the D0– vs D0+ comparisons. There were statistically significant ( P <0.05) increases in haemolysis percentage, red cell distribution width (RDW) percentage and mean OF, and decreases in packed cell volume (PCV), RBC count, haemoglobin and haematocrit for D0– vs D35–. The same was found for D0– vs D35+ with the addition of a significant increase in mean cell haemoglobin (MCH). For D35– vs D35+ only MCH significantly increased. At day 35, 6/9 units had haemolysis percentages that exceeded 1%. This increased to 8/9 of stored units post-filter passage. All blood units cultured negative. Conclusions and relevance Fresh RBCs exhibited no in vitro evidence of injury following passage through an 18 μm microaggregate filter. Increased MCH was observed in the stored blood and may represent haemolysis induced by the filter. All other changes can be explained by storage lesion rather than filter passage. The findings highlight the importance of blood banking quality controls and the need for further research to assess the effects of transfusion technique, specifically filter passage, on storage lesion-affected feline blood.

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“…Numerous studies have examined storage-based changes in rheological properties of RBCs and have shown decreased deformability in RBCs associated with oxidative stress 31 and increased mean corpuscular hemoglobin concentration 32 . In addition, the recent study by Alshalani et al emphasized the importance of monitoring water permeability as a potential predictor to assess the quality of the RBCs during hypothermic storage 33 . Using QPM, we analyzed RBCs flowing through constrictive microfluidic channels after different lengths of storage time.…”

Section: Discussionmentioning

confidence: 99%

Quantitative phase imaging of erythrocytes under microfluidic constriction in a high refractive index medium reveals water content changes

et al. 2019

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Changes in the deformability of red blood cells can reveal a range of pathologies. For example, cells which have been stored for transfusion are known to exhibit progressively impaired deformability. Thus, this aspect of red blood cells has been characterized previously using a range of techniques. In this paper, we show a novel approach for examining the biophysical response of the cells with quantitative phase imaging. Specifically, optical volume changes are observed as the cells transit restrictive channels of a microfluidic chip in a high refractive index medium. The optical volume changes indicate an increase of cell’s internal density, ostensibly due to water displacement. Here, we characterize these changes over time for red blood cells from two subjects. By storage day 29, a significant decrease in the magnitude of optical volume change in response to mechanical stress was witnessed. The exchange of water with the environment due to mechanical stress is seen to modulate with storage time, suggesting a potential means for studying cell storage.

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Impact of blood manufacturing and donor characteristics on membrane water permeability and in vitro quality parameters during hypothermic storage of red blood cells

Cited by 15 publications

References 30 publications

The State of the Red Blood Cell Membrane in Iron Deficiency Anaemia in Women of Reproductive Age

The State of the Red Blood Cell Membrane in Iron Deficiency Anaemia in Women of Reproductive Age

Effect of microaggregate filter passage on feline whole blood stored for 35 days

Quantitative phase imaging of erythrocytes under microfluidic constriction in a high refractive index medium reveals water content changes

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