Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

Pishchik, V. N.; Vorobyov, N. I.; Ostankova, Yu. V.; Semenov, A. G.; Арег, А Тотолян; Popov, A. A.; Khomyakov, Yu. V.; Удалова, О. Р.; Shibanov, D; Vertebny, V. Eu.; Dubovitskaya, V. I.; Sviridova, Olga; Walsh, Olga S.; Shafian, Sanaz

doi:10.9734/ijpss/2018/41148

Cited by 13 publications

(8 citation statements)

References 34 publications

(40 reference statements)

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“…The highest amounts of individual and total organic acids were recorded in fully irrigated plants treated with TW, whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. This finding coincides with the report of Pishchik et al [58], who suggested that the application of Bacillus subtilis had a beneficial effect on the organic acid content of tomato fruit. In general, organic acid content decreased under water stress for all the biostimulant treatments compared to the respective treatments of fully irrigated plants, which suggests the small contribution of organic acids as osmoprotectants under stress.…”

Section: Resultssupporting

confidence: 92%

“…The only exception was recorded in the untreated plants that received full irrigation, where chlorophyll b and total chlorophyll content was significantly lower than under specific treatments (e.g., TWW+, CW-and XSW-). Several reports suggest the beneficial effects of microbial biostimulants on the leaf chlorophyll content of various vegetables [58,64,[66][67][68][69], whereas, in fruit, this effect is expected to be lessened due to the gradual degradation of chlorophyll with ripening progression [70].…”

Section: Resultsmentioning

confidence: 99%

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Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit

et al. 2022

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The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit

et al. 2022

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“…Consequently, substantial efforts have been extended to the use of physiochemical and ecofriendly biological approaches, which are currently being applied to alleviate plant heat stress. Recently, the co-administration of HA and the Bacillus genus as important plant bio-stimulants that improve the growth and productivity in different crops [41,49] while reducing the dependency on chemical fertilizers has begun to gain importance. Previous reports demonstrated that humic substances may favor bacteria-plant interactions and enhance the bacterial attachment and colonization [47,74].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, Busato et al [48] applied a microbial suspension and a humic substance to plant substrates to promote seedling adaptation to stressful environments. Pishchik et al [49] reported the impact of combined application of microbes and HA on tomato plants. However, least information are available on the combined effects of PGPB specifically endophytes and HA on tomato seedlings under heat stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Extending thermotolerance to tomato seedlings by inoculation with SA1 isolate of Bacillus cereus and comparison with exogenous humic acid application

et al. 2020

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Heat stress is one of the major abiotic stresses that impair plant growth and crop productivity. Plant growth-promoting endophytic bacteria (PGPEB) and humic acid (HA) are used as biostimulants and ecofriendly approaches to improve agriculture crop production and counteract the negative effects of heat stress. Current study aimed to analyze the effect of thermotolerant SA1 an isolate of Bacillus cereus and HA on tomato seedlings. The results showed that combine application of SA1+HA significantly improved the biomass and chlorophyll fluorescence of tomato plants under normal and heat stress conditions. Heat stress increased abscisic acid (ABA) and reduced salicylic acid (SA) content; however, combined application of SA1+HA markedly reduced ABA and increased SA. Antioxidant enzymes activities revealed that SA1 and HA treated plants exhibited increased levels of ascorbate peroxidase (APX), superoxide dismutase (SOD), and reduced glutathione (GSH). In addition, heat stress markedly reduced the amino acid contents; however, the amino acids were increased with co-application of SA1 +HA. Similarly, inductively-coupled plasma mass-spectrometry results showed that plants treated with SA1+HA exhibited significantly higher iron (Fe + ), phosphorus (P), and potassium (K + ) uptake during heat stress. Heat stress increased the relative expression of SlWRKY33b and autophagy-related (SlATG5) genes, whereas co-application of SA1+HA augmented the heat stress response and reduced SlWRKY33b and SlATG5 expression. The heat stressresponsive transcription factor (SlHsfA1a) and high-affinity potassium transporter (SlHKT1) were upregulated in SA1+HA-treated plants. In conclusion, current findings suggest that coapplication with SA1+HA can be used for the mitigation of heat stress damage in tomato plants and can be commercialized as a biofertilizer.

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“…The 1.5-kilobase partial sequence of the 16S rRNA gene was amplified using a polymerase chain reaction (PCR) and universal Eubacteria-specific primers: 8F (5 -AGAGTTTGATCCTGGCTCAG-3 ) and 1541R (5 -AAGGAGGTGATCCAGCCGCA-3 ). PCR experiments were conducted according to the protocol described in [95]. The PCR product was sequenced using the two primers given above and the following set of two forward and two reverse primers: 805R (5 -GGACTACCAGGGTATCTAATCCC-3 ), 515R (5 -GWATTACCGCGGCKGCTG-3 ), 508F (5 -AACTACGTGCCAGCAGC-3 ), 908F (5 -AAACTCAAAGGAATTGACGG-3 ).…”

Section: S Rrna Sequencing Of Pgpbmentioning

confidence: 99%

Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress

Pishchik

Filippova

Mirskaya

et al. 2021

Plants

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The present study demonstrates the Ni toxicity-ameliorating and growth-promoting abilities of two different bacterial isolates when applied to wheat (Triticum aestivum L.) as the host plant. Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected based on their ability to improve the germination of wheat plants; they were identified as Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2. The protective effects of these epiphytic bacteria against Ni stress were studied in model experiments with two wheat cultivars: Ni stress-tolerant Leningradskaya 6 and susceptible Chinese spring. When these isolates were used as the inoculants applied to Ni-treated wheat plants, the growth parameters and the levels of photosynthetic pigments of the two wheat cultivars both under normal and Ni-stress conditions were increased, though B. megaterium AFI1 had a more pronounced ameliorative effect on the Ni contents in plant tissues due to its synthesis of siderophores. Over the 10 days of Ni exposure, the plant growth promotion bacteria (PGPB) significantly reduced the lipid peroxidation (LPO), ascorbate peroxidase (APX), superoxide dismutase (SOD) activities and proline content in the leaves of both wheat cultivars. The PGPB also increased peroxidase (POX) activity and the levels of chlorophyll a, chlorophyll b, and carotenoids in the wheat leaves. It was concluded that B. megaterium AFI1 is an ideal candidate for bioremediation and wheat growth promotion against Ni-induced oxidative stress, as it increases photosynthetic pigment contents, induces the antioxidant defense system, and lowers Ni metal uptake.

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Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

Cited by 13 publications

References 34 publications

Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit

Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit

Extending thermotolerance to tomato seedlings by inoculation with SA1 isolate of Bacillus cereus and comparison with exogenous humic acid application

Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress

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