Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress

Alharby, Hesham F.; Metwali, E. M. R.; Fuller, Michael P.; Aldhebiani, Amal Y.

doi:10.2298/abs151105017a

Cited by 112 publications

(46 citation statements)

References 43 publications

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“…The color and texture of callus also elucidated few prominent changes. Similarly, previous studies have revealed that increasing the concentration of ZnO NPs causes rise of CI and/or CFW . Studies of Alharby et al.…”

Section: Discussionmentioning

confidence: 63%

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Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress

Mosavat

Golkar

Yousefifard

et al. 2019

Biotech and App Biochem

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Thymus species are aromatic plants with diverse applications in food industries and medicine. This study was conducted to evaluate the potential effect of ZnO nanoparticles (NPs) on callus proliferation and thymol and carvacrol production in three Thymus species, that is, T. vulgaris, T. daenensis, and T. kotschyanus, and Zataria multiflora. For this purpose, callus induction was performed on Murashige and Skoog (MS) medium containing different plant growth regulators (PGRs). After optimization of callus growth, the effects of different concentrations of ZnO NPs (100 and 150 mg L −1 ) were investigated. MS containing 2 mg L −1 of 2, 4-dichlorophenoxy acetic acid (2,4-D) and 1 mg L −1 of kinetin (Kin) revealed significantly highest fresh weight (0.18 g) of callus in T.kotschyanus. Callus growth rate (0.079 mm day −1 ) was found highest in T. vulgaris under similar conditions. Moreover, highest callus induction (92.50%) was achieved by T. kotschyanus in MS containing 2.5 mg L −1 of 2,4-D. Regarding the highest content of thymol (22.8 mg L −1 ) and carvacrol (0.68 mg L −1 ) evaluated by high-performance liquid chromatography, best results were achieved under 150 mg L −1 of ZnO NPs in T. kotschyanus and T. daenesis, respectively. This is simple and cost-effective method to be applied on industrial level for production of enhanced secondary metabolites content. potent chemical elicitors as signaling agents that effect a great diversity of physiological and biochemical processes in plant metabolism and plant secondary metabolites production [1,2]. NPs are capable of increasing cell membrane permeability, stimulating defensive mechanisms in plant cells, and subsequent increase in secondary metabolites content [2,3]. Zinc oxide (ZnO) NPs as an inorganic micronutrient have a beneficial effect on plant growth, cell division, and structure and function of membrane because of their high electron mobility, large surface area, and catalytic activity [4]. Most of the recent studies are focused on the effects of NPs on seed germination [5], plant growth rate [6] physiological responses [3][4][5][6], and secondary metabolites production [2][3][4][5]. Recent studies have shown that plant growth, development, and physiology are significantly affected by NPs; however, their role on plant secondary metabolism, especially regarding callus culture, is still obscure [2]. The inclusion of NPs to tissue culture media could enhance

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Section: Discussionmentioning

confidence: 63%

“…Studies of Alharby et al. revealed that higher concentrations of ZnO NPs treatments, that is, 30 mg L −1 as compared with 15 mg L −1 enhanced callus growth of Solanum lycopersicum Mill. In another study, Javed et al.…”

Section: Discussionmentioning

confidence: 99%

Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress

Mosavat

Golkar

Yousefifard

et al. 2019

Biotech and App Biochem

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“…The Moringa peregrine plants sprayed with Hogland solution containing ZnO NPs showed an increase in growth parameters either under saline or normal condition (Soliman et al, 2015). Further, Alharby et al (2016) showed that the ZnO NPs could mitigate salt stress in tomato callus culture in vitro at concentration 15 mg/l. Du et al (2011) found that ZnO NPs have higher solubility than TiO2 NPs, dissolved in the soil, and increased the root wheat uptake of excessive zinc.…”

Section: Discussionmentioning

confidence: 94%

Improving date palm (phoenix dactylifera l. cv. estamaran) calogenesis by the use of zinc oxide nanoparticles

Amiri¹,

Mousavi²,

Torahi³

2016

JEBAS

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To study the effects of zinc nanoparticles on date palm cv. Estamaran (Sayer) callus induction under in vitro condition, a research with two separate experiments was conducted using a completely randomized design. In the first experiment, MS basal salt medium containing 100 mg/l 2,4-D, 3 mg/l 2ip, 3 g/l activated charcoal, 30 g/l sucrose, 40 mg/l adenine sulfate, and 1 mg/l biotin along with different concentrations of zinc sulfate (ZnSO4), including 0, 2.43, 4.86 mg/l or zinc oxide nanoparticles (ZnO NPs), including 0, 2.43, 4.86, 7.29 mg/l , were used. While in the second experiment, the effect of various medium strengths including full strength MS, ½ MS, and ¼ MS was examined on callus formation. At the end of each experiment, total explants induced callus, embryogenic callus formation percentage, and callus fresh and dry weight were evaluated. Results of the first experiment showed that the best callus induction and callus fresh and dry weight belong to MS medium containing 2.43 mg/l ZnO NPs. Further, higher ZnO NPs concentrations had negative effects on callus formation. However, results of the second experiment indicated that highest callus formation and callus fresh and dry weight are obtained on full strength MS basal salt medium.

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“…L À1 NaCl. 59 ZnO NPs mitigated the effects of NaCl by upregulating antioxidant enzymes like GPX and SOD. In Solanum nigrum, the frequency of callus formation (89%) and the fresh weight of the callus (4.67 g per leaf explant) were increased on MS medium augmented with 5 mg L À1 BA, 3 mg L À1 NAA and 8 mg L À1 Ag NPs.…”

Section: Input Of Nanomaterials Towards Callus Induction Organogenesmentioning

confidence: 98%

Nanomaterials in plant tissue culture: the disclosed and undisclosed

2017

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Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress

Cited by 112 publications

References 43 publications

Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress

Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress

Improving date palm (phoenix dactylifera l. cv. estamaran) calogenesis by the use of zinc oxide nanoparticles

Nanomaterials in plant tissue culture: the disclosed and undisclosed

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