Impact of anatase titanium dioxide nanoparticles on mutagenic and genotoxic response in Chinese hamster lung fibroblast cells (V-79): The role of cellular uptake

Jain, Abhishek Kumar; Senapati, Violet Aileen; Singh, Divya; Dubey, Kavita; Maurya, Ranjana; Pandey, Alok Kumar

doi:10.1016/j.fct.2017.04.005

Cited by 35 publications

(31 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neither were there detected abnormalities in the cell cycle nor increased cell death after exposure to these particles, visible in vesicles in the cellular cytoplasm 72 h after exposure [57][58][59][60]. Furthermore, some TiO 2 nanoparticle aggregates were observed in the centrosomal region of the cells, as previously reported [61]. Interestingly, these nanoparticles can remain encapsulated in membranes for long periods of time (24-48 h) without any evidence of their exocytosis [60].…”

Section: Liposomessupporting

confidence: 68%

Effect of Size, Shape, and Composition on the Interaction of Different Nanomaterials with HeLa Cells

Renero-Lecuna

Iturrioz-Rodríguez

González-Lavado

et al. 2019

Journal of Nanomaterials

View full text Add to dashboard Cite

The application of nanomaterials in the fields of medicine and biotechnology is of enormous interest, particularly in the areas where traditional solutions have failed. Unfortunately, there is very little information on how to optimize the preparation of nanomaterials for their use in cell culture and on the effects that these can trigger on standard cellular systems. These data are pivotal in nanobiotechnology for the development of different applications and to evaluate/compare the cytotoxicity among the different nanomaterials or studies. The lack of information drives many laboratories to waste resources performing redundant comparative tests that often lead to partial answers due to differences in (i) the nature of the start-up material, (ii) the preparation, (iii) functionalization, (iv) resuspension, (v) the stability/dose of the nanomaterial, etc. These variations in addition to the different analytical systems contribute to the artefactual interpretation of the effects of nanomaterials and to inconsistent conclusions between different laboratories. Here, we present a brief review of a wide range of nanomaterials (nanotubes, various nanoparticles, graphene oxide, and liposomes) with HeLa cells as a reference cellular system. These human cells, widely used as cellular models for many studies, represent a reference system for comparative studies between different nanomaterials or conditions and, in the last term, between different laboratories.

show abstract

Section: Liposomessupporting

confidence: 68%

Effect of Size, Shape, and Composition on the Interaction of Different Nanomaterials with HeLa Cells

Renero-Lecuna

Iturrioz-Rodríguez

González-Lavado

et al. 2019

Journal of Nanomaterials

View full text Add to dashboard Cite

show abstract

“…TiO2 is found in three crystalline phase (rutile, anatase and brookit) but only the first two crystal structure are used in industry. Several studies have shown different toxicological responses of cells depending on the crystal form in different cell lines (Saquib et al 2012;Gerloff et al 2012;Wang et al 2015;Uboldi et al 2016;Jain et al 2017), and in most cases, anatase phase exhibited the highest toxicity compared to the others. Among the described cytotoxic effects induced by TiO2-NPs in different cells lines, ROS generation, DNA damage (Saquib et al 2012;Wang et al 2015), apoptosis, cell cycle arrest (Wu et al 2 2010; Kansara et al 2015) and increased inflammatory response (Han et al 2005), are the most common.…”

Section: Introductionmentioning

confidence: 99%

Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes

Montalvo-Quirós

Luque-García

2019

Food and Chemical Toxicology

View full text Add to dashboard Cite

“…After exposure, treatment was removed, and then the cells were washed and harvested with trypsin. The pellet was collected and cells were reseeded again in DMEM with 10% FBS for 1 week to allow the mutation to be expressed, during which time the cells were passaged three times as described earlier (Chen et al, ; Doak et al, ; Jain et al, ) with slight modifications. Afterwards, cells were harvested and grown at a density of 2.5 × 10 5 cells per Petri dish in freshly prepared selective medium containing 6TG.…”

Section: Methodsmentioning

confidence: 99%

“…After exposure, treatment was removed, and then the cells were washed and harvested with trypsin. The pellet was collected and cells were reseeded again in DMEM with 10% FBS for 1 week to allow the mutation to be expressed, during which time the cells were passaged three times as described earlier (Chen et al, 2014;Doak et al, 2012;Jain et al, 2017) In brief, the V-79 cells were grown on a six-well culture plate at a density of 2.0 × 10 5 cells/well/mL of complete culture medium (DMEM + 10% FBS) at 37°C, 5% CO 2 . Thereafter, cells were exposed to different concentrations of ZnO NPs (0-20 μg/mL) for 6 hours.…”

Section: Mutagenicity Assessment (Hgprt Gene Forward Mutation Assay)mentioning

confidence: 99%

Zinc oxide nanoparticles induced gene mutation at the HGPRT locus and cell cycle arrest associated with apoptosis in V‐79 cells

Jain

Singh

Dubey

et al. 2019

J of Applied Toxicology

Self Cite

View full text Add to dashboard Cite

In recent years, the large‐scale production of ZnO nanoparticles (NPs) for various applications is increasing exponentially and may pose serious health issues when inhaled either during occupational exposure or in consumer settings. The mechanisms underlying the toxicity of NPs have recently been studied intensively. Despite the existing studies, the mutagenicity of ZnO NPs in the eukaryotic system is still unclear. Therefore, the aim of the present study was to investigate the mutagenic potential of ZnO NPs using Chinese hamster lung fibroblast cells (V‐79) as an in‐vitro model. The study has demonstrated a significant uptake of ZnO NPs by flow cytometry with the confirmation of transmission electron microscopy. A reduction in cell viability was observed with a concomitant increase in reactive oxygen species (**P < 0.01, ***P < 0.001) after ZnO NP (1‐20 μg/mL) exposure. Excessive reactive oxygen species can induce oxidative stress, which leads to genotoxic insult, and further gene mutation. Apart from measuring the genotoxicity by Comet assay, a change of 2.84‐fold in the HGPRT gene mutant frequency was observed by the mammalian gene forward mutation assay. All the genotoxicity endpoints such as chromosomal break, DNA damage and mutagenicity were observed at 6 hours of ZnO NP exposure. Our results also showed that ZnO NPs manifested the cell cycle arrest, ultrastructural modifications and further cell death. A significant (**P < 0.01, ***P < 0.001) increase in the apoptotic cells was detected using annexin V‐fluorescein isothiocyanate/propidium iodide double staining by flow cytometry. Our findings presented here clearly stimulate the need for careful regulations of ZnO NPs.

show abstract

Impact of anatase titanium dioxide nanoparticles on mutagenic and genotoxic response in Chinese hamster lung fibroblast cells (V-79): The role of cellular uptake

Cited by 35 publications

References 51 publications

Effect of Size, Shape, and Composition on the Interaction of Different Nanomaterials with HeLa Cells

Effect of Size, Shape, and Composition on the Interaction of Different Nanomaterials with HeLa Cells

Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes

Zinc oxide nanoparticles induced gene mutation at the HGPRT locus and cell cycle arrest associated with apoptosis in V‐79 cells

Contact Info

Product

Resources

About