IMPACT OF Allium sativum AGAINST Enterobacter sp. AS WATER BORNE PATHGENIC BACTERIA ISOLATED FROM RIVER NILE

Abstract

To explore the antibacterial activities of Allium sativium (garlic) extract was tested against two waterborne pathogenic strains isolated from River Nile, to mitigate the increase of bacterial resistance to conventional antibiotics. The two isolates were identified as Enterobacter cloacae DSM 3264 BRB & Enterobacter cloacae MB11506_1CHB by MALDI-Tof-MS. Aqueous, methanolic and oil extraction of garlic were tested for their inhibitory activity against the selected strains using well diffusion method. Enterobacter sp. were more sensitive towards oil extract with inhibition zone 2.3 cm rather than aqueous and methanolic extractions with was 50%. Analysis of garlic essential oil by GC-MS dedicated six sulfur compounds represented 25% of total detected compounds in the oil.

Inhibitory potentials of Streptomyces exfoliatus strain ‘MUJA10’ against bacterial pathogens isolated from rural areas in Riyadh, Saudi Arabia

“…Five ml of peptone water were inoculated by 3–5 single colonies of the selected isolate and incubated at 37 °C for 24 h. Thereafter, optical density (OD) of the culture was adjusted to 0.06–0.8 using the spectrophotometer at 625 nm which is equivalent to (14×10 6 CFU/ml) to prepare the standard inoculum [ 29 ].…”