Impact of agglomeration and different dispersions of titanium dioxide nanoparticles on the human related in vitro cytotoxicity and genotoxicity

Magdolénová, Zuzana; Bilaničová, Dagmar; Pojana, Giulio; Fjellsbø, Lise Marie; Hudecová, Alexandra; Hašplová, Katarína; Marcomini, Antonio; Dušinská, Maria

doi:10.1039/c2em10746e

Cited by 140 publications

(111 citation statements)

References 34 publications

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“…For example, cytotoxicity and genotoxicity of TiO 2 NPs were dependent on the state of the NPs aggregation. 18 Unstabilized NPs tend to agglomerate, while stabilized NPs are more dispersed. 19 Also, the chemical composition of the surface coating of the NPs is another relevant factor.…”

Section: Discussionmentioning

confidence: 99%

Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles

Schütz¹,

Staedler²,

Crosbie-Staunton³

et al. 2014

IJN

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Therapeutic engineered nanoparticles (NPs), including ultrasmall superparamagnetic iron oxide (USPIO) NPs, may accumulate in the lower digestive tract following ingestion or injection. In order to evaluate the reaction of human colon cells to USPIO NPs, the effects of non-stabilized USPIO NPs (NS-USPIO NPs), oleic-acid-stabilized USPIO NPs (OA-USPIO NPs), and free oleic acid (OA) were compared in human HT29 and CaCo 2 colon epithelial cancer cells. First the biophysical characteristics of NS-USPIO NPs and OA-USPIO NPs in water, in cell culture medium supplemented with fetal calf serum, and in cell culture medium preconditioned by HT29 and CaCo 2 cells were determined. Then, stress responses of the cells were evaluated following exposure to NS-USPIO NPs, OA-USPIO NPs, and free OA. No modification of the cytoskeletal actin network was observed. Cell response to stress, including markers of apoptosis and DNA repair, oxidative stress and degradative/autophagic stress, induction of heat shock protein, or lipid metabolism was determined in cells exposed to the two NPs. Induction of an autophagic response was observed in the two cell lines for both NPs but not free OA, while the other stress responses were cell- and NP-specific. The formation of lipid vacuoles/droplets was demonstrated in HT29 and CaCo 2 cells exposed to OA-USPIO NPs but not to NS-USPIO NPs, and to a much lower level in cells exposed to equimolar concentrations of free OA. Therefore, the induction of lipid vacuoles in colon cells exposed to OA utilized as a stabilizer for USPIO NPs is higly amplified compared to free OA, and is not observed in the absence of this lipid in NS-USPIO NPs.

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Section: Discussionmentioning

confidence: 99%

Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles

Schütz¹,

Staedler²,

Crosbie-Staunton³

et al. 2014

IJN

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“…Photogenotoxic effects of NMs in combination with ultraviolet radiation have been measured with the comet assay (Jha, 2008). The possibility of interference of NMs in the assay -causing additional damage to DNA during the performance of the assay, or inhibiting enzyme activity -has been discussed (Magdolenova et al, 2012;Karlsson et al, 2015) but the evidence to date suggests that these concerns are unfounded and the comet assay is a reliable test, suitable for adaptation as a highthroughput assay.…”

Section: Dna Damagementioning

confidence: 99%

“…This is partly due to different intrinsic properties of NMs, but more importantly, to distinct secondary characteristics related to cell culture medium and dispersion methods, which may have a huge impact on the results of the studies. In addition, inconsistent testing conditions, with NMs in different physiological media (biological fluids and tissues), could affect kinetics, distribution and interactions of the NMs with biological components (Kato et al, 2009;Magdolenova et al, 2012;Guadagnini et al, 2015). It is also important to identify and study degradation of the NMs in the biological environment, as the release of molecular debris could induce cytotoxic effects (Treuel and Nienhaus, 2012).…”

Section: Characterization Of Nmsmentioning

confidence: 99%

Immunotoxicity, genotoxicity and epigenetic toxicity of nanomaterials: New strategies for toxicity testing?

Dušinská

Tulinská

Yamani

et al. 2017

Food and Chemical Toxicology

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112

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a b s t r a c tThe unique properties of nanomaterials (NMs) are beneficial in numerous industrial and medical applications. However, they could also induce unintended effects. Thus, a proper strategy for toxicity testing is essential in human hazard and risk assessment. Toxicity can be tested in vivo and in vitro; in compliance with the 3Rs, alternative strategies for in vitro testing should be further developed for NMs. Robust, standardized methods are of great importance in nanotoxicology, with comprehensive material characterization and uptake as an integral part of the testing strategy. Oxidative stress has been shown to be an underlying mechanism of possible toxicity of NMs, causing both immunotoxicity and genotoxicity. For testing NMs in vitro, a battery of tests should be performed on cells of human origin, either cell lines or primary cells, in conditions as close as possible to an in vivo situation. Novel toxicity pathways, particularly epigenetic modification, should be assessed along with conventional toxicity testing methods. However, to initiate epigenetic toxicity screens for NM exposure, there is a need to better understand their adverse effects on the epigenome, to identify robust and reproducible causal links between exposure, epigenetic changes and adverse phenotypic endpoints, and to develop improved assays to monitor epigenetic toxicity.

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“…For a proper biological hazard assessment, dispersion in culture medium has to be carefully monitored (Magdolenova et al 2012). Here, size distribution of aggregates was measured by centrifugal sedimentation in the different testing conditions adopted for the biological tests.…”

Section: Aggregation In Cell Culturementioning

confidence: 99%

Inhibition of the ROS-mediated cytotoxicity and genotoxicity of nano-TiO2 toward human keratinocyte cells by iron doping

et al. 2014

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Inhibition of the ROS-mediated cytotoxicity and genotoxicity of nano-TiO 2 toward human keratinocyte cells by iron doping J Nanopart Res (2014) Nano-TiO 2 powders are widely used in sunscreen lotions as UV filters in combination with other substances. The activation of TiO 2 by UV rays leads to the release of reactive oxygen species (ROS, e.g., hydroxyl radicals and singlet oxygen) which are potentially harmful. For this reason the TiO 2 particles are generally coated with inert materials (e.g., silica or alumina) that inhibit such reactivity. Alternatively, the release of ROS may be inhibited by introducing in the TiO 2 lattice doping elements. In the present study we report a new modification consisting in a wet impregnation of TiO 2 with iron salts followed by a thermal treatment that results in an inhibition of the surface reactivity. The insertion of iron ions also gradually reduces the ability of photo-activated TiO 2 to cleave DNA and proteins. At the same time, a clear inhibition of cyto-and geno-toxicity toward human (HaCaT) keratinocytes was observed. The data presented herein suggest the insertion of Fe 3+ ions at the surface of nano-TiO 2 as a promising strategy to reduce the photo-induced toxicity of nano-TiO 2 powders.

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Impact of agglomeration and different dispersions of titanium dioxide nanoparticles on the human related in vitro cytotoxicity and genotoxicity

Cited by 140 publications

References 34 publications

Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles

Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles

Immunotoxicity, genotoxicity and epigenetic toxicity of nanomaterials: New strategies for toxicity testing?

Inhibition of the ROS-mediated cytotoxicity and genotoxicity of nano-TiO2 toward human keratinocyte cells by iron doping

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