Inhibition of the ROS-mediated cytotoxicity and genotoxicity of nano-TiO2 toward human keratinocyte cells by iron doping

Ghiazza, Mara; Alloa, Elisa; Oliaro‐Bosso, Simonetta; Viola, Franca; Livraghi, Stefano; Rembges, Diana; Capomaccio, Robin; Rossi, François; Ponti, Jessica; Fenoglio, Ivana

doi:10.1007/s11051-014-2263-z

Cited by 19 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EPR spin trapping investigations of reactive radicals produced in the irradiated TiO 2 dispersions so far were focused mainly on aqueous systems and on the detection of hydroxyl radicals [20,31,35,36,37,49]. Analogous experiments performed in organic solvents may provide interesting information concerning the radical intermediates generated [13,71,72,73,74], consequently we carried out spin trapping experiments with TiO 2 nanoparticles dispersed in DMSO, acetonitrile (ACN), methanol and ethanol.…”

Section: Resultsmentioning

confidence: 99%

“…EPR spectroscopy occupies an exclusive position in the investigation of titania photocatalysts, providing a characterization of paramagnetic centers produced via the trapped photogenerated electrons and holes [29,30,31,32,33,34,35,36], and of titanium dioxide materials with transition-metal ions doping [17,34]. A majority of the research exploiting EPR spectroscopy deals with the investigation of reactive radical intermediates produced in the irradiated TiO 2 particulate systems where the application of an indirect spin trapping technique is inevitable [37,38,39,40,41,42,43,44,45].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Radical Intermediates in Photoinduced Reactions on TiO2 (An EPR Spin Trapping Study)

2014

View full text Add to dashboard Cite

Abstract:The radical intermediates formed upon UVA irradiation of titanium dioxide suspensions in aqueous and non-aqueous environments were investigated applying the EPR spin trapping technique. The results showed that the generation of reactive species and their consecutive reactions are influenced by the solvent properties (e.g., polarity, solubility of molecular oxygen, rate constant for the reaction of hydroxyl radicals with the solvent). The formation of hydroxyl radicals, evidenced as the corresponding spin-adducts, dominated in the irradiated TiO2 aqueous suspensions. The addition of 17 O-enriched water caused changes in the EPR spectra reflecting the interaction of an unpaired electron with the 17 O nucleus. The photoexcitation of TiO2 in non-aqueous solvents (dimethylsulfoxide, acetonitrile, methanol and ethanol) in the presence of 5,5-dimethyl-1-pyrroline N-oxide spin trap displayed a stabilization of the superoxide radical anions generated via electron transfer reaction to molecular oxygen, and various oxygen-and carbon-centered radicals from the solvents were generated. The character and origin of the carbon-centered spin-adducts was confirmed using nitroso spin trapping agents.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radical Intermediates in Photoinduced Reactions on TiO2 (An EPR Spin Trapping Study)

2014

View full text Add to dashboard Cite

show abstract

“…2B). This signal indicates the presence of the radical TEMPONE that is formed via the reaction between singlet oxygen and the probe 4-oxo-TMP [44][45][46].…”

Section: Identification Of Ros Produced By the Sonodynamic Or Photodymentioning

confidence: 99%

Insight into ultrasound-mediated reactive oxygen species generation by various metal-porphyrin complexes

Giuntini

Foglietta

Marucco

et al. 2018

Free Radical Biology and Medicine

Self Cite

View full text Add to dashboard Cite

Ultrasound is used to trigger the cytotoxicity of chemical compounds, known as sonosensitisers, in an approach called sonodynamic therapy (SDT), which is under investigation herein. The generation of reactive oxygen species (ROS) has been proposed as the main biological occurrence that leads to the cytotoxic effects, which are achieved via the synergistic action of two components: the energy-absorbing sonosensitiser and ultrasound (US), which are both harmless per se. Despite some promising results, a lack of investigation into the mechanisms behind US sonosensitiser-mediated ROS generation has prevented SDT from reaching its full potential. The aim of this work is to investigate the US-responsiveness of a variety of metal-porphyrin complexes, free-base porphyrin and Fe(III), Zn(II) and Pd(II) porphyrin, by analyzing their ROS generation under US exposure and related bio-effects. All experiments were also carried out under light exposure and the results were used as references. Our results show that porphyrin ultrasound-responsiveness depends on the metal ion present, with Zn(II) and Pd(II) porphyrin being the most efficient in generating singlet oxygen and hydroxyl radicals. ROS production efficiency is lower after ultrasound exposure than after light exposure, because of the various physico-chemical mechanisms involved in sensitiser activation. US and porphyrin-mediated ROS generation is oxygen-dependent and the activation of porphyrin by US appears to be more compatible with sonoluminescence-based photo-activation rather than a radical path process that occurs via the homolytic bond rupture of water. Notably, the cytotoxicity results reported herein, which are mirrored by ex-cellulo data, confirm that the type of ROS generation achieved by the US activation of intracellular porphyrins is pivotal to the effectiveness of cancer cell killing.

show abstract

“…These results suggest that NAC has some potential as an antidote for nano-TiO 2 phototoxicity (Xue et al 2011). Furthermore, Ghiazza et al (2014) found that the ROS-mediated cytotoxicity and genotoxicity of nano-TiO 2 toward human keratinocyte cells could be inhibited by iron doping. They suggest that impregnation with iron salts might be a promising strategy to reduce this kind of cytotoxicity and genotoxicity.…”

Section: Toxicity Of Nano-tio 2 On Skinmentioning

confidence: 84%

Toxicology of nanosized titanium dioxide: an update

2015

View full text Add to dashboard Cite

Nanosized titanium dioxide (nano-TiO2) has tremendous potential for a host of applications, and TiO2 nanoparticles (NP) possess different physicochemical properties compared to their fine particle analogs, which might alter their bioactivity. Their adverse effects on living cells have raised serious concerns recently for their use in health care and consumer sectors such as sunscreens, cosmetics, pharmaceutical additives and implanted biomaterials. Many researches have demonstrated that the physicochemical properties including shape, size, surface characteristics and inner structure of nano-TiO2 particles have different degrees of toxicity to different organism groups under different conditions. Some former reports have demonstrated that nano-TiO2 materials could enter into human body through different routes such as inhalation, dermal penetration and ingestion. After being taken by human body, NP might induce oxidative stress, cytotoxicity, genotoxicity, inflammation and cell apoptosis ultimately in mammal organs and systems. Here, we summarized the update about toxicity of nano-TiO2 and aimed to supply a safety usage guideline of this nanomaterial.

show abstract

Inhibition of the ROS-mediated cytotoxicity and genotoxicity of nano-TiO2 toward human keratinocyte cells by iron doping

Cited by 19 publications

References 52 publications

Radical Intermediates in Photoinduced Reactions on TiO2 (An EPR Spin Trapping Study)

Radical Intermediates in Photoinduced Reactions on TiO2 (An EPR Spin Trapping Study)

Insight into ultrasound-mediated reactive oxygen species generation by various metal-porphyrin complexes

Toxicology of nanosized titanium dioxide: an update

Contact Info

Product

Resources

About