Impact of Adhesive Application to Wet and Dry Dentin on Long-term Resin-dentin Bond Strengths

Reis, Alessandra; Pellizzaro, Arlete; Dal-Bianco, Karen; Gómes, Osnara María Mongruel; Patzlaff, Rafael Tiago; Loguércio, AD

doi:10.2341/06-107

Cited by 93 publications

(85 citation statements)

References 33 publications

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“…During SE bonding, dissolved hydroxyapatite crystals are incorporated into the hybridized complex, and, depending on the pH of the adhesive system, the whole extension of demineralized dentin is impregnated by resin monomers (2). For this reason, the SE strategy has not been considered highly sensitive to moisture and drying (7). However, although the number of clinical steps is reduced with SE systems, the longevity of the restoration may be compromised compared with that produced by ER systems, especially when simplified versions of these adhesives (one-step systems) are used (8).…”

Section: Introductionmentioning

confidence: 99%

Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

et al. 2018

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This study evaluated application protocol (etch-and-rinse/ER and self-etching/SE) and dentin wettability (wet and dry) on microtensile bond strength (mTBS) and transdentinal cytotoxicity of Scotchbond TM Universal (SU) adhesive system. The mTBS values and fracture mode were registered 24 h after adhesive system application and resin composite block build-up (n=5). For analysis of transdentinal cytotoxicity, odontoblast-like MDPC-23 cells were seeded on pulpal surface of dentin discs (0.4 mm thick) adapted to artificial pulp chambers (n=8). The adhesive system was applied to occlusal surface, followed by 24-h incubation time. Cell viability (Alamar Blue) and morphology (SEM) were assessed. Adper Single Bond 2 and Clearfil SE Bond were used as positive controls of the ER and SE application protocols, respectively. No treatment was performed on negative control (NC) group. Data were analyzed by ANOVA and Tukey's tests (α=5%). Higher mTBS values were found for ER mode in comparison with SE protocol (p<0.05). Dentin wettability had no effect on bond strength of SU in both the ER and SE techniques (p>0.05). Most fractures involved hybrid layer and/or adhesive layer. Neither variable prevented the intense toxic effects of adhesive systems on MDPC-23 cultured cells, since intense reduction in cell viability (±88%) and severe alterations in cell morphology were observed for all groups compared to NC, with no differences among them (p>0.05). Therefore, it was concluded that application of SU following the ER protocol had better adhesive performance. However, this adhesive system featured intense transdentinal cytotoxicity to pulp cells, regardless of application protocol and dentin wettability.

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Section: Introductionmentioning

confidence: 99%

Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

et al. 2018

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“…11 Chan and others found bond strength to dentin with a thick smear layer increased significantly with agitation and, on SEM evaluation, they noted passive application resulted in a hybridized smear layer, while agitation resulted in the smear layer being completely dissolved or dispersed into the adhesive. 11 Reis and others 41 concluded that the rubbing action can improve the kinetics and allow for better monomer diffusion inward, while solvents are diffusing outward, which may explain the high bond strength values obtained under slight and vigorous agitation. It is likely that vigorous agitation improved the removal of residual water, which increased the degree of conversion and cross-linking of the polymer and, consequently, the mechanical properties of the resin inside the hybrid layer.…”

Section: Discussionmentioning

confidence: 99%

Effect of Placement Agitation and Drying Time on Dentin Shear Bond Strength: An In Vivo Study

Tewari¹,

Goel²

2009

Operative Dentistry

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The current study measured the bond strength of a self-etch system to dentin with and without agitation and with varying drying times of primer in vital dentin. The null hypotheses tested were that primer agitation and primer drying time did not affect the dentin shear bond strength. Sixty human maxillary and mandibular premolars scheduled to be extracted for orthodontic reasons were selected. The adhesive/resin combination used was Clearfil SE (Kuraray, Osaka, Japan)/TPH Spectrum (Dentsply DeTrey, Konstanz, Germany). The occlusal surfaces of the teeth were flattened using straight fissure diamond abrasive points ISO-012. Samples were divided into six groups of 10 teeth each. Primer was applied following the manufacturer's instructions with and without agitation followed by air-drying time of 0 (without air drying), five and 10 seconds. Clearfil SE Bond was applied and cured for 10 seconds. TPH Spectrum composite, shade A2 (Dentsply DeTrey), was placed over cured adhesive and was cured for 40 seconds. The teeth were restored to their original anatomy. The teeth were extracted after one week and the samples were kept in distilled water until testing at room temperature. The samples were tested in shear at a 1 mm/minute crosshead speed using an LR100 Universal Testing Machine. The data was analyzed using ANOVA and Tukey post-hoc tests (p<0. Clinical RelevancePrimer agitation and primer drying time both affect dentin shear bond strength in vivo.shear bond strength to dentin. Agitation showed no effect when primer was not dried or it was dried for 10 seconds.

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“…In most in vitro tests, there are variations in the period of storage, which can be 24 hours, a week, two weeks, 3, 6 or 12 months [18,11,19]. The storage time in the present study was 24 hours.…”

Section: Discussionmentioning

confidence: 99%

“…The scientific literature contains studies reporting a reduction in infiltration based on the use of adhesive systems [11][12][13][14][15][16][17][18][19][20] over a short-term assessment period. Thus, shortterm results are material-dependent, given that long-term assessments have shown that the adhesive is not the dominant factor in terms of reducing infiltration around amalgam restorations [11].…”

Section: Discussionmentioning

confidence: 99%

Universal Adhesive Systems in Class II Amalgam Restorations

Nascimento

Lima

Souza

et al. 2016

Rev. Odonto Ciênc. (Online)

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Objective: To assess the effectiveness of adhesive system in reducing microleakage in class II amalgam restorations. Methods: The teeth were divided randomly into 3 equal groups: Group I was the control experiment (Copal Vanish); Group II: single Bond Universal 3M-ESPE (with and without acid -37%); and Group III: All Bond Universal -BISCO (with and without acid -37%), after the amalgam was condensed. The root apices were then sealed with acrylic resin and stored for 24 hours in 100% humidity. After storage, the specimens were subjected to 500 cycles of thermocycling (5°C and 55°C). The teeth were isolated with cosmetic nail polish and were immersed in 0.5 basic fuchsin dye for 24 hours. The teeth then were sectioned with a diamond disc and observed under a stereomicroscope. Microleakage was assessed using a 0-3 scale of dye penetration. The data was analyzed using the Kruskal-Wallis test (p<0.05). Results: The microleakage scores revealed less leakage in the experimental groups than in the control group. The microleakage for the adhesives without acid conditioning was reduced, although no statistical significance was recorded (p<0.05). Conclusion: The immediate adhesive material acted effectively as a barrier for microleakage when it was applied without acid.Key words: Adhesives; Universal adhesive; Amalgams; Microleakage; Class II Sistema adesivo universal em restaurações de amálgama classe II RESUMO Objetivo: Avaliar a eficácia do sistema adesivo na redução da microinfiltração em restaurações de amálgama classe II. Metodologia: Os dentes foram divididos aleatoriamente em três grupos iguais: Grupo I foi o controle (Copal Vanish); Grupo II: Single Bond Universal 3M ESPE -(com e sem ácido -37%); e Grupo III: All bond Universal -BISCO (com e sem ácido -37%) com posterior condessação do amálgama. Os ápices foram então seladas com resina acrílica e armazenado durante 24 horas em 100% de humidade. Após a armazenagem, as amostras foram submetidas a 500 ciclos de termociclagem (5°C e 55°C). Os dentes foram isolados com esmalte para unha e foram imersos em 0,5 de fucsina básica por 24 horas. Em seguida os dentes foram seccionados com disco de diamante e observados sob microscópio estereoscópico. A microinfiltração foi avaliada usando uma escala de penetração do corante de 0-3. Os dados foram analisados utilizando o teste de Kruskal-Wallis (p<0,05). Resultados: Os escores revelaram menor infiltração nos grupos experimentais do que no grupo de controle. A infiltração para os adesivos sem condicionamento ácido foi reduzida, embora sem significância estatística (p<0,05). Conclusão: O material adesivo imediato atuou efetivamente como uma barreira para microinfiltração, quando foi aplicada sem condicionamento ácido.

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Impact of Adhesive Application to Wet and Dry Dentin on Long-term Resin-dentin Bond Strengths

Cited by 93 publications

References 33 publications

Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

Effect of Placement Agitation and Drying Time on Dentin Shear Bond Strength: An In Vivo Study

Universal Adhesive Systems in Class II Amalgam Restorations

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