Immunotoxicological impact and biodistribution assessment of bismuth selenide (Bi2Se3) nanoparticles following intratracheal instillation in mice

Mishra, Vani; Baranwal, Vikas; Mishra, Rohit Kumar; Sharma, Shivesh; Paul, Bhola Nath; Pandey, Avinash C.

doi:10.1038/s41598-017-18126-y

Cited by 20 publications

(12 citation statements)

References 66 publications

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“…According to the crystallographic spectrum, all of the diffraction peaks can be attributed to the pure rhombohedral phase of Bi (JCPDS No. 05-0519), which is consistent with previous research [11,30]. The most intense peak at 2h = 27.23 is obtained along (012) reflection plane and it is very close to the position of the strongest line (2h = 29.32) of the reference bismuth [30].…”

Section: Resultssupporting

confidence: 91%

“…05-0519), which is consistent with previous research [11,30]. The most intense peak at 2h = 27.23 is obtained along (012) reflection plane and it is very close to the position of the strongest line (2h = 29.32) of the reference bismuth [30]. This XRD pattern suggests that the final product is elemental bismuth, not bismuth oxide, given the current synthetic circumstances.…”

Section: Resultssupporting

confidence: 89%

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Quaternary Trimethyl Chitosan Chloride Capped Bismuth Nanoparticles with Positive Surface Charges: Catalytic and Antibacterial Activities

et al. 2021

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Quaternary trimethyl chitosan-stabilized bismuth nanoparticles (QTMC-BiNPs) with positive surface charges were uniquely synthesized and fully characterized. In the synthesis, Quaternary Trimethyl Chitosan (QTMC), a water-soluble derivative of chitosan (CTS) was prepared using two-step reductive methylation. The new biopolymeric functionalized ligand was further used as capping agent for the synthesis of QTMC-BiNPs which was applied as antibacterial and catalytic agents. The reaction was carried out at room temperature without the use of energy consuming or high-cost instruments. The QTMC and nanocomposites were characterized by proton nuclear magnetic resonance ( 1 H NMR), attenuated total reflection Fourier-transform infrared, UV-visible, X-ray diffraction, X-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopic techniques. The topology and morphology of the composites were examined with scanning electron microscopy and high-resolution transmission electron microscopy. Thermogravimetric and differential thermal gravimetric analysis were also conducted. The degree of quaternization and degree of dimethylation values of 63.33 and 11.75%, respectively obtained for QTMC confirmed that the main product is a quaternary derivative. The average particle size of QTMC-BiNPs was evaluated to be between 30 and 45 nm. The QTMC-BiNPs revealed clear and uniform lattice fringes with an estimated interplanar d-spacing of 0.32 nm confirming the formation of highly crystalline nanocomposites. A further insight into the antibacterial activities of this nanomaterial were carefully examined on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) using resazurin based microdilution method for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The obtained results revealed that both bacteria pathogens were effectively inhibited/killed by the QTMC-BiNPs at very low concentrations. The MIC of 15.63 and 125 lg/mL were recorded against E. coli and S. aureus, respectively while the MBC of 31.25 and 500.00 lg/mL were estimated against E. coli and S. aureus, respectively. An extensive evaluation of the catalytic capability of the nanocomposites towards the reduction of 4-nitrophenol to 4-aminophenol was also carried out with highly promising result.Keywords Chitosan Á Quaternary trimethyl chitosan Á Bismuth nanoparticles Á Antibacterial activity Á Catalytic activity

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 89%

Quaternary Trimethyl Chitosan Chloride Capped Bismuth Nanoparticles with Positive Surface Charges: Catalytic and Antibacterial Activities

et al. 2021

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“…The hydropathicity value of the final vaccine is predicted to be − 0.521. This negative value suggests that our final vaccine is hydrophilic in nature and can interact with water molecules easily 62 . The charge is 37.00; this value will decrease in alkaline environment so usually it is better if the charge values are positive.…”

Section: Resultsmentioning

confidence: 99%

An in silico deep learning approach to multi-epitope vaccine design: a SARS-CoV-2 case study

Yang

Bogdan

Nazarian

2021

Sci Rep

156

111

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The rampant spread of COVID-19, an infectious disease caused by SARS-CoV-2, all over the world has led to over millions of deaths, and devastated the social, financial and political entities around the world. Without an existing effective medical therapy, vaccines are urgently needed to avoid the spread of this disease. In this study, we propose an in silico deep learning approach for prediction and design of a multi-epitope vaccine (DeepVacPred). By combining the in silico immunoinformatics and deep neural network strategies, the DeepVacPred computational framework directly predicts 26 potential vaccine subunits from the available SARS-CoV-2 spike protein sequence. We further use in silico methods to investigate the linear B-cell epitopes, Cytotoxic T Lymphocytes (CTL) epitopes, Helper T Lymphocytes (HTL) epitopes in the 26 subunit candidates and identify the best 11 of them to construct a multi-epitope vaccine for SARS-CoV-2 virus. The human population coverage, antigenicity, allergenicity, toxicity, physicochemical properties and secondary structure of the designed vaccine are evaluated via state-of-the-art bioinformatic approaches, showing good quality of the designed vaccine. The 3D structure of the designed vaccine is predicted, refined and validated by in silico tools. Finally, we optimize and insert the codon sequence into a plasmid to ensure the cloning and expression efficiency. In conclusion, this proposed artificial intelligence (AI) based vaccine discovery framework accelerates the vaccine design process and constructs a 694aa multi-epitope vaccine containing 16 B-cell epitopes, 82 CTL epitopes and 89 HTL epitopes, which is promising to fight the SARS-CoV-2 viral infection and can be further evaluated in clinical studies. Moreover, we trace the RNA mutations of the SARS-CoV-2 and ensure that the designed vaccine can tackle the recent RNA mutations of the virus.

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“…These results in conjunction with other study parameters, suggest no major sign of respiratory, metabolic, or kidney disorders in SPA4 peptide-treated mice. Other published research studies have also investigated parameters of toxicity in other non-pulmonary organs of animals intratracheally treated with different agents, such as statin [36], vascular endothelial growth factorsmall-interfering RNA [37], iron oxide and bismuth selenide nanoparticles [38,39], ,and amphotericin B formulation [40].…”

Section: Discussionmentioning

confidence: 99%

Lung and general health effects of Toll-like receptor-4 (TLR4)-interacting SPA4 peptide

et al. 2020

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Background: A surfactant protein-A-derived peptide, which we call SPA4 peptide (amino acids: GDFRYSDGTPVNYTNWYRGE), alleviates lung infection and inflammation. This study investigated the effects of intratracheally administered SPA4 peptide on systemic, lung, and health parameters in an outbred mouse strain, and in an intratracheal lipopolysaccharide (LPS) challenge model. Methods: The outbred CD-1 mice were intratracheally administered with incremental doses of SPA4 peptide (0.625-10 μg/g body weight) once every 24 h, for 3 days. Mice left untreated and those treated with vehicle were included as controls. Mice were euthanized after 24 h of last administration of SPA4 peptide. In order to assess the biological activity of SPA4 peptide, C57BL6 mice were intratracheally challenged with 5 μg LPS/g body weight and treated with 50 μg SPA4 peptide via intratracheal route 1 h post LPS-challenge. Mice were euthanized after 4 h of LPS challenge. Signs of sickness and body weights were regularly monitored. At the time of necropsy, blood and major organs were harvested. Blood gas and electrolytes, serum biochemical profiles and SPA4 peptide-specific immunoglobulin G (IgG) antibody levels, and common lung injury markers (levels of total protein, albumin, and lactate, lactate dehydrogenase activity, and lung wet/dry weight ratios) were determined. Lung, liver, spleen, kidney, heart, and intestine were examined histologically. Differences in measured parameters were analyzed among study groups by analysis of variance test. Results: The results demonstrated no signs of sickness or changes in body weight over 3 days of treatment with various doses of SPA4 peptide. It did not induce any major toxicity or IgG antibody response to SPA4 peptide. The SPA4 peptide treatment also did not affect blood gas, electrolytes, or serum biochemistry. There was no evidence of injury to the tissues and organs. However, the SPA4 peptide suppressed the LPS-induced lung inflammation. Conclusions: These findings provide an initial toxicity profile of SPA4 peptide. Intratracheal administration of escalating doses of SPA4 peptide does not induce any significant toxicity at tissue and organ levels. However, treatment with a dose of 50 μg SPA4 peptide, comparable to 2.5 μg/g body weight, alleviates LPS-induced lung inflammation.

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Immunotoxicological impact and biodistribution assessment of bismuth selenide (Bi2Se3) nanoparticles following intratracheal instillation in mice

Cited by 20 publications

References 66 publications

Quaternary Trimethyl Chitosan Chloride Capped Bismuth Nanoparticles with Positive Surface Charges: Catalytic and Antibacterial Activities

Quaternary Trimethyl Chitosan Chloride Capped Bismuth Nanoparticles with Positive Surface Charges: Catalytic and Antibacterial Activities

An in silico deep learning approach to multi-epitope vaccine design: a SARS-CoV-2 case study

Lung and general health effects of Toll-like receptor-4 (TLR4)-interacting SPA4 peptide

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