Immunosuppressive flavones and lignans from Bupleurum scorzonerifolium

Chang, Wen-Liang; Chiu, Li-Wen; Lai, Jenn‐Haung; Lin, Hang-Ching

doi:10.1016/j.phytochem.2003.08.002

Cited by 40 publications

(26 citation statements)

References 18 publications

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“…The acetone extract AE-BS was prepared as described previously [25,32] . The AE-BS was dissolved in 95% MeOH solution and then partitioned (1:1) with n-hexane to give the n-hexane-soluble fraction (BS-HE).…”

Section: Methodsmentioning

confidence: 99%

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Lin²,

et al. 2010

Acta Pharmacol Sin

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Aim: To investigate the effect of isochaihulactone (also known as K8), a lignan compound of Bupleurum scorzonerifolium, on H 2 O 2 -induced cytotoxicity in neuronally differentiated PC12 cells (nPC12). Methods: Viability of neuronal PC12 cells was measured using MTT assay. Protein expression was determined by Western blot. Apoptotic cells was determined using TUNEL assay. D-galactose aging mice were used as a model system to study the anti-oxidant effects of isochaihulactone in vivo. Results: Pretreatment with isochaihulactone (5-10 μmol/L) increased cell viability and decreased membrane damage, generation of reactive oxygen species and degradation of poly (ADP-ribose) polymerase in H 2 O 2 -treated nPC12 cells and also decreased the expression of cyclooxygenase-2, via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. The results suggest that isochaihulactone is a potential antioxidant agent. In a murine aging model, in which chronic systemic exposure to D-galactose (D-gal) causes the acceleration of senescence, administration of isochaihulactone (10 mg·kg -1 ·d -1 , sc) for 7 weeks concomitant with D-gal injection significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma. Furthermore, H&E staining to quantify cell death within hippocampus showed that percentage of pyknotic nuclei in the D-gal-treated mice were much higher than in control. Conclusion: The results suggest that isochaihulactone exerts potent anti-aging effects against D-gal in mice possibly via antioxidative mechanisms.

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Section: Methodsmentioning

confidence: 99%

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Lin²,

et al. 2010

Acta Pharmacol Sin

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“…The isolated compounds wogonin, eugenin and saikochromone A inhibited the secretion at a dose of 10 mg/ml by 99, 72 and 71%, respectively. [50] The acidic polysaccharide fraction from B. smithii inhibited the complement activation (IC50 340 and 81 mg/ml) for both classical and alternative pathways, respectively. This inhibition was mediated through interaction with C1s, C3 and C4 complements, which are the major components of the innate immune system.…”

Section: Immunomodulatory Activitymentioning

confidence: 96%

“…[102] Buddlejasaponin I, IV and the sulfated saikosaponin (sandrosaponin I), which were isolated from B. rigidum, exhibited a very potent in-vivo anti-inflammatory effect (1 mg/ear dose) on mouse ear oedema induced by PMA compared with the equivalent dose of indometacin. The effect of these compounds on other inflammatory parameters [109] Rotundioside F (-) Acute inflammation [109] Rotundioside A, H, I, J Cytotoxic against MK-1, HeLa and B16F10 cell lines [30,110] B. salicifolium Polyacetylenes derivatives Antibacterial [111,112] B. scorzonerifolium Acetone extract Cytotoxic against A549 cell line [113][114][115] Wogonin, eugenin, saikochromone A (-) IL-2 [50] Saikosaponin B3, bupleurosides III, VI, IX and XIII and scorzonerosides A, B, C…”

Section: B Rigidummentioning

confidence: 99%

Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

Ashour

Wink

2010

Journal of Pharmacy and Pharmacology

225

161

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Objectives Radix Bupleuri represents one of the most successful and widely used herbal drugs in Asia for treatment of many diseases over the past 2000 years. Thorough studies have been carried out on many species of this genus and have generated immense data about the chemical composition and corresponding biological activity of extracts and isolated secondary metabolites. In this work, we review the chemistry and pharmacology of the genus Bupleurum and explore the relationships between the pharmacological effects and the chemical composition of these drugs. Key findings Early studies on the genus Bupleurum had focused only on the traditional uses of the plants in the treatment of inflammatory disorders and infectious diseases. After chemical profiling, several groups of secondary metabolites were characterized with relevant biological activity: triterpene saponins (saikosaponins), lignans, essential oils and polysaccharides. As a result, present interest is now focused on the bioactivity of the isolated triterpene saponins acting as immunomodulatory, anti-inflammatory and antiviral agents, as well as on the observed ant-iulcer activity of the polysaccharides and anti-proliferative activity of different lignans. Many saikosaponins exhibited very potent anti-inflammatory, hepatoprotective and immunomodulatory activities both in vivo and in vitro. Conclusions Further investigations and screenings are required to explore other Bupleurum species, to evaluate the clinical safety and possible interactions with other drugs or herbs. Standardization of Bupleuri extracts is crucial for them being integrated into conventional medicine due to large chemical and biological variations between different species and varieties.

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“…17) Therefore, the absolute configuration of C-3 of compound 1 opposite that of isochaihulactone and should be 3S. Consequently, the structure of compound 1 was elucidated as (3S,6Z)-2-(3Ј,4Ј,5Ј-trimethoxybenzylidene)-3-(3Љ,4Љ-methylenedioxybenzyl)-g-butyrolactone.…”

Section: Resultsmentioning

confidence: 99%

Lignans and Coumarins from the Roots of Anthriscus sylvestris and Their Increase of Caspase-3 Activity in HL-60 Cells

Jeong

Kwon

Park

et al. 2007

Biological & Pharmaceutical Bulletin

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Immunosuppressive flavones and lignans from Bupleurum scorzonerifolium

Cited by 40 publications

References 18 publications

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

Lignans and Coumarins from the Roots of Anthriscus sylvestris and Their Increase of Caspase-3 Activity in HL-60 Cells

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