Immunostimulatory Effects of Blueberry Yeast Fermented Powder Against Cyclophosphamide-induced Immunosuppressed Model

Jeong, Do Youn; Yang, Hee-Jong; Jeong, Su Ji; Kim, Min-Kook; Yun, Chi Young; Lee, Hak Yong; Lee, Yang Hee; Shin, Dong Yeop; Yang, Yea gin; Lee, Hae Seong; Park, Young Mi

doi:10.15188/kjopp.2019.02.33.1.48

Cited by 4 publications

(4 citation statements)

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“…Immune function is essential to maintain health because it constitutes the defenses against infection. If immune function is lowered, the body becomes vulnerable to various infections; the effects of lowered immune function are an important clinical problem worldwide [ 24 ]. Various studies have been conducted to determine ways to improve immune function and identify natural materials without side effects [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Immune-Enhancing Effect of Submerged Culture of Ceriporia lacerata Mycelia on Cyclophosphamide-Induced Immunosuppressed Mice and the Underlying Mechanisms in Macrophages

Hwang¹,

Lee

Pham

et al. 2022

IJMS

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The white-rot fungi Ceriporia lacerata is used in bioremediation, such as lignocellulose degradation, in nature. Submerged cultures and extracts of C. lacerata mycelia (CLM) have been reported to contain various active ingredients, including β-glucan and extracellular polysaccharides, and to exert anti-diabetogenic properties in mice and cell lines. However, the immunostimulatory effects have not yet been reported. This study aimed to identify the immunomodulatory effects, and underlying mechanisms thereof, of submerged cultures of CLM using RAW264.7 macrophages and cyclophosphamide (CTX)-induced immunosuppression in mice. Compared to CTX-induced immunosuppressed mice, the spleen and thymus indexes in mice orally administered CLM were significantly increased; body weight loss was alleviated; and natural killer (NK) cytotoxicity, lymphocyte proliferation, and cytokine (tumor necrosis factor [TNF]-α, interferon [IFN]-γ, and interleukin [IL]-2) production were elevated in the serum. In RAW264.7 macrophages, treatment with CLM induced phagocytic activity, increased the production of nitric oxide (NO), and promoted mRNA expression of the immunomodulatory cytokines TNF-α, IFN-γ, IL-1β, IL-6, IL-10, and IL-12. In addition, CLM increased the inducible NO synthase (iNOS) concentration in macrophages, similar to lipopolysaccharide (LPS) stimulation. Mechanistic studies showed that CLM induced the activation of the NF-κB, PI3k/Akt, ERK1/2, and JNK1/2 pathways. Moreover, the phosphorylation of NF-κB and IκB induced by CLM in RAW264.7 cells was suppressed by specific MAPKs and PI3K inhibitors. Further experiments with a TLR4 inhibitor demonstrated that the production of TNF-α, IL-1β, and IL-6 induced by CLM was decreased after TLR4 was blocked. Overall, CLM protected against CTX-induced adverse reactions by enhancing humoral and cellular immune functions, and has potential as an immunomodulatory agent.

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Section: Discussionmentioning

confidence: 99%

Immune-Enhancing Effect of Submerged Culture of Ceriporia lacerata Mycelia on Cyclophosphamide-Induced Immunosuppressed Mice and the Underlying Mechanisms in Macrophages

Hwang¹,

Lee

Pham

et al. 2022

IJMS

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“…Antioxidant [204 -206], Immunomodulant [206,207], Antiviral [208], Anticancer [208], 208], 206], active against Circulatory disorder [208], Hypercholesterolemia and Hypertension [206].…”

Section: Related Previous Reportsmentioning

confidence: 99%

Anti-COVID-19 Biomedicines - A Layout Proposal for Production, Storage and Transportation

Pattanayak¹

2021

TOCOVIDJ

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Background: Modulation of non-specific immunity and other related activities of succulent parts of effective medicinal plants can prevent viral infections like COVID-19 through their dietary intake. Objective: The succulent parts of the medicinal plants with immunomodulation, anti-oxidation, anti-viral, anti-inflammatory, etc. power can be used orally in the capsular form to prevent as well as to reduce the severity of symptoms of COVID-19. Methods: A proposal is displayed with a detailed description of related steps like the selection of medicinal plant parts consulting related reports, collection of biomedicines, validation of efficacy, dosing, encapsulation, storage, and transportation, etc. Results: The succulent bio-medicines against COVID-19 can be developed and marketed following only some adoptive research. Conclusion: Succulent bio-medicines can be prepared and marketed for the prevention and cure of different infectious and non-infectious diseases.

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“…The immune system plays a crucial defense-mechanism role by preventing pathogens and various infectious agents from initiating disease [ 1 ]. Homeostasis in the immune system is maintained by immunostimulation and immunosuppression, leading to immune regulation [ 2 ]. This defense system classically comprises the two sectors of innate and adaptive immunity [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Immune-Enhancing Effects of Lactobacillus plantarum 200655 Isolated from Korean Kimchi in a Cyclophosphamide-Induced Immunocompromised Mouse Model

Kim

Paik

Kim

2021

J. Microbiol. Biotechnol.

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The immune system plays a crucial defense-mechanism role by preventing pathogens and various infectious agents from initiating disease [1]. Homeostasis in the immune system is maintained by immunostimulation and immunosuppression, leading to immune regulation [2]. This defense system classically comprises the two sectors of innate and adaptive immunity [3]. In the non-specific defenses, known as innate immunity, neutrophils, macrophages, natural killer (NK) cells, and dendritic cells perceive exogenous factors and promote the production of cytokines [4]. When macrophages are activated, they eliminate the hazard directly by phagocytosis and indirectly by releasing inflammatory cytokines such as nitric oxide (NO), prostaglandin E 2 (PGE 2 ), tumor necrosis factor-α (TNF-α), and the interleukins . In contrast, the specific defenses, known as adaptive immunity, are characterized by T and B cells [6]. Inflammatory cytokines are also accompanied by the NF-κB and MAPK signaling pathways [7], which are mediated by toll-like receptors (TLRs) [8]. Physiological and pathological responses, including stress responses, inflammation, and apoptosis, are mediated by the MAPK signaling pathway in mammalian cells and tissues [9]. In the MAPK signaling pathway, phosphorylation of p38 MAPK and ERK1/2 plays important roles, activating the production of TNF-α, IL-6, and IL-8, which are inflammatory factors [10]. Moreover, the MAPK cascade and IL-8 secretion are triggered by . In addition to MAPK, NF-κB regulates the expression of proinflammatory mediators. NF-κB p65 protein combines with IκB protein and exists in the cytoplasm. Depending on external stimuli, NF-κB kinases (IKKs) can phosphorylate the IκB family of proteins, followed by ubiquitination and degradation, further phosphorylating p65 and releasing it into the nucleus, where it promotes inflammation.Cyclophosphamide (CP) is a widely used alkylating cytotoxic agent that exerts broad-spectrum activities in modulating immune responses against various illnesses [1,12]. However, administration of high-dose CP can cause immunosuppression and intestinal complications [13]. CP-induced immunosuppression decreases the number In this study, we evaluated the immune-enhancing activity of kimchi-derivedLactobacillus plantarum 200655 on immune suppression by cyclophosphamide (CP) in ICR mice. Animals were fed distilled water or 1×10 9 colony-forming unit/kg B.W. 200655 or Lactobacillus rhamnosus GG as a positive control for 14 days. An in vivo model of immunosuppression was induced using CP 150 and 100 mg/kg B.W. at 7 and 10 days, respectively. Body weight, spleen index, spleen weight, and gene expression were measured to estimate the immune-enhancing effects. The dead 200655 (D-200655) group showed an increased spleen weight compared to the sham control (SC) group. Similarly, the spleen index was significantly higher than that in the CP-treated group. The live 200655 (L-200655) group showed an increased mRNA expression of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 in splen...

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Immunostimulatory Effects of Blueberry Yeast Fermented Powder Against Cyclophosphamide-induced Immunosuppressed Model

Cited by 4 publications

References 0 publications

Immune-Enhancing Effect of Submerged Culture of Ceriporia lacerata Mycelia on Cyclophosphamide-Induced Immunosuppressed Mice and the Underlying Mechanisms in Macrophages

Immune-Enhancing Effect of Submerged Culture of Ceriporia lacerata Mycelia on Cyclophosphamide-Induced Immunosuppressed Mice and the Underlying Mechanisms in Macrophages

Anti-COVID-19 Biomedicines - A Layout Proposal for Production, Storage and Transportation

Immune-Enhancing Effects of Lactobacillus plantarum 200655 Isolated from Korean Kimchi in a Cyclophosphamide-Induced Immunocompromised Mouse Model

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